Phenotypes Associated with This Genotype

Genotype
MGI:3037198

• Allelic Composition: Srsf2^tm1Xdfu/Srsf2^tm1Xdfu; Tg(Lck-cre)I57Jxm/?
• Genetic Background: involves: 129S4/SvJae * 129X1/SvJ * ICR

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

absent thymus corticomedullary boundary ( J:88668 )

• in histological sections the cortical-medullary junction is absent

small thymus ( J:88668 )

• the size and weight of the thymus is about 10% to 30% that in wild-types

• other peripheral lymphoid organs appear normal

abnormal T cell differentiation ( J:88668 )

• in T cells sorted by stage cells with the gene deleted are progressively eliminated

• splicing of CD45 is abnormal with a significant percentage of single positive and double positive T cells expressing CD45 isoforms missing exon 5

increased double-negative T cell number ( J:88668 )

• the percentage of double-negative T cells could be elevated up to 40% of the total (compared to 6% in wild-type)

• T cell development is arrested at the CD44^-CD25⁺ stage

decreased T cell number ( J:88668 )

• the absolute number of single positive T cells is greatly reduced

decreased thymocyte number ( J:88668 )

• a dramatic reduction in total thymocytes is seen

decreased double-positive T cell number ( J:88668 )

• the percentage of double-positive T cells could be decreased down to 50% of the total (compared to 85% in wild-type)

abnormal spleen white pulp morphology ( J:88668 )

• the white pulp is paler than normal

• the absolute number of single positive T cells is significantly reduced

• none of the mature T cells found in the spleen have the gene deleted

• the spleen is otherwise unaffected

immune system

absent thymus corticomedullary boundary ( J:88668 )

• in histological sections the cortical-medullary junction is absent

small thymus ( J:88668 )

• the size and weight of the thymus is about 10% to 30% that in wild-types

• other peripheral lymphoid organs appear normal

abnormal T cell differentiation ( J:88668 )

• in T cells sorted by stage cells with the gene deleted are progressively eliminated

• splicing of CD45 is abnormal with a significant percentage of single positive and double positive T cells expressing CD45 isoforms missing exon 5

increased double-negative T cell number ( J:88668 )

• the percentage of double-negative T cells could be elevated up to 40% of the total (compared to 6% in wild-type)

• T cell development is arrested at the CD44^-CD25⁺ stage

decreased double-positive T cell number ( J:88668 )

• the percentage of double-positive T cells could be decreased down to 50% of the total (compared to 85% in wild-type)

decreased T cell number ( J:88668 )

• the absolute number of single positive T cells is greatly reduced

decreased thymocyte number ( J:88668 )

• a dramatic reduction in total thymocytes is seen

abnormal spleen white pulp morphology ( J:88668 )

• the white pulp is paler than normal

• the absolute number of single positive T cells is significantly reduced

• none of the mature T cells found in the spleen have the gene deleted

• the spleen is otherwise unaffected

endocrine/exocrine glands

absent thymus corticomedullary boundary ( J:88668 )

• in histological sections the cortical-medullary junction is absent

small thymus ( J:88668 )

• the size and weight of the thymus is about 10% to 30% that in wild-types

• other peripheral lymphoid organs appear normal

decreased thymocyte number ( J:88668 )

• a dramatic reduction in total thymocytes is seen