Analysis Tools
renal/urinary system
|
• urinary Ca2+/Cr ratios are decreased unlike in human patients with homozygous loss of ATP6V1B1 where calcium levels are increased
|
|
• in mice on a standard chow diet (pH of 7.2 compared to 6.1 in wild-type controls)
• after acid loading urine is markedly more alkaline than in similarly treated wild-type controls
• furosemide treatment fails to increase urine acidity
|
|
• the rate of intracellular pH recovery in intercalcated cells is markedly reduced after cellular acidification but is not affected by concanamycin treatment
|
homeostasis/metabolism
|
• decrease in venous plasma bicarbonate level following acid loading
|
|
• urinary Ca2+/Cr ratios are decreased unlike in human patients with homozygous loss of ATP6V1B1 where calcium levels are increased
|
|
• following acid loading (1.5% NH4Cl/1% sucrose in water), acidosis is more severe in mutants compared to wild-type mice
• however, unlike in human patients with homozygous loss of ATP6V1B1 systemic acidosis is not detected in mice on a standard chow diet
|
|
• in mice on a standard chow diet (pH of 7.2 compared to 6.1 in wild-type controls)
• after acid loading urine is markedly more alkaline than in similarly treated wild-type controls
• furosemide treatment fails to increase urine acidity
|
hearing/vestibular/ear
| N |
• at 5-8 months of age, homozygotes exhibit a normal Preyer's reflex and do not display circling/waltzing behaviors or other signs of vestibular dysfunction
• inner ear histology, auditory brainstem responses, and endocochlear potentials are unaffected, indicating normal inner ear function
|
skeleton
| N |
• bone density is normal
|
