Phenotypes Associated with This Genotype

Genotype
MGI:2670955

• Allelic Composition: Ptx3^tm1Zuk/Ptx3^tm1Zuk
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased susceptibility to Coronaviridae infection ( J:188421 )

• mice show increased susceptibility to infection with the coronavirus murine hepatitis virus strain 1 (MHV-1) , showing more severe lung injury in the lungs in the earlier stages of infection but not in later stages

• MHV-1 infected mice show increased early inflammatory cell infiltration, with higher neutrophil and macrophage recruitment in the lungs at 1-2 days post infection compared to controls

• treatment of MHV-1-infected mice with recombinant PTX3 attenuates virus-induced lung injury and blocks neutrophil infiltration in the lungs but promotes pulmonary infiltration of macrophages

reproductive system

abnormal cumulus oophorus morphology ( J:76877 )

♀ • ovaries isolated from female homozygotes at 8 hrs post-hCG are enriched in preovulatory follicles; however, in several preovulatory follicles, the oocyte is not surrounded by cumulus cells or the expanded cumulus mass appears to be randomly scattered in the antrum surrounding the oocyte

absent cumulus expansion ( J:76877 )

♀ • after hormonal (hCG) stimulation, wild-type oocytes are embedded in a three-dimensional extracellular matrix, whereas mutant oocytes are denuded of cumulus granulosa cells that readily detach from the cumulus oophorus

decreased superovulation rate ( J:76877 )

♀ • at 19 to 20 hrs after hCG stimulation, fewer oocytes are released into the oviducts of homozygous mutant females (9.9 0.5) relative to heterozygous (31.2 0.6) or wild-type females (22.2 0.9)

reduced female fertility ( J:76877 )

♀ • Background Sensitivity: on a mixed genetic background, female homozygotes bred to wild-type males over a 6-month period exhibit significantly reduced fertility, whereas on a 129/SvEv inbred genetic background, female homozygotes fail to produce live offspring

decreased litter size ( J:76877 )

• female homozygotes produce fewer pups born per litter and nearly half the number of litters per month relative to heterozygous females (2.24 0.16 vs 8.16 0.11 pups/litter and 0.59 0.07 vs 1.02 0.03 litter/month, respectively)

impaired fertilization ( J:76877 )

• only 1.3% of oocytes isolated from superovulated female homozygotes are able to reach the two-cell stage relative to 55% of oocytes from wild-type females

endocrine/exocrine glands

abnormal cumulus oophorus morphology ( J:76877 )

♀ • ovaries isolated from female homozygotes at 8 hrs post-hCG are enriched in preovulatory follicles; however, in several preovulatory follicles, the oocyte is not surrounded by cumulus cells or the expanded cumulus mass appears to be randomly scattered in the antrum surrounding the oocyte

absent cumulus expansion ( J:76877 )

♀ • after hormonal (hCG) stimulation, wild-type oocytes are embedded in a three-dimensional extracellular matrix, whereas mutant oocytes are denuded of cumulus granulosa cells that readily detach from the cumulus oophorus