Phenotypes Associated with This Genotype

Genotype
MGI:2669562

• Allelic Composition: Rad51^tm1Hst/Rad51^tm1Hst
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:36540 )

• embryos die between E5.5 to E7.5

• at E7.5, embryos are beginning to resorb

embryo

absent inner cell mass proliferation ( J:36540 )

• mutant cells derived from the inner cell mass failed to proliferate in vitro

embryonic growth arrest ( J:36540 )

• growth arrest at the egg cylinder stage (E5.5)

decreased embryo size ( J:36540 )

• at E7.5, embryos are smaller than controls

abnormal embryonic tissue morphology ( J:36540 )

• homozygous embryos are described as hypocellular with cuboidal cell morphology

increased embryonic epiblast cell apoptosis ( J:36540 )

• apoptotic cells are frequently observed in epiblasts of E7.5 mutant embryos

absent mesoderm ( J:36540 )

absent amniotic cavity ( J:36540 )

• amniotic cavity fails to develop

growth/size/body

decreased embryo size ( J:36540 )

• at E7.5, embryos are smaller than controls

cellular

increased cellular sensitivity to gamma-irradiation ( J:36540 )

• trophoblast cells in culture are reduced in number and fewer cells attach to culture plates after exposure to gamma irradiation

increased embryonic epiblast cell apoptosis ( J:36540 )

• apoptotic cells are frequently observed in epiblasts of E7.5 mutant embryos

decreased cell proliferation ( J:36540 )

• mutant embryos incorporate less BrdU at E5.5 and E6.5 indicated reduced cellular proliferation

absent inner cell mass proliferation ( J:36540 )

• mutant cells derived from the inner cell mass failed to proliferate in vitro