Analysis Tools
mortality/aging
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• homozygous mutant embryos die between E7-E10.5
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embryo
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• at E9.5, 9 of 9 homozygotes fail to complete axial turning
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• at E9.5, homozygotes are smaller than wild-type embryos
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• at E9.5, homozygotes display an open neural tube with blood cells in the headfold (probably secondary consequences of heart defect)
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• at E8.5 and E9.5, ~70% of homozygotes retain an open proamniotic canal, leading to chorion malformation
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• at E8.5 and E9.5, ~70% of homozygotes retain an open proamniotic canal
• by E10.5, 2 of 2 homozygotes exhibit an open proamniotic canal
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• at E8.5 and E9.5, all homozygotes display delayed allantois development
• in some cases, the mutant allantois fails to reach the ectoplacental cone
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cardiovascular system
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• at E8.5 and E9.5, 2 of 12 homozygotes display no signs of heart development, despite an apparently normal amnion/chorion formation
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• at E8.5, homozygotes display delayed cardiac development relative to wild-type embryos
• at E9.5, homozygotes still exhibit a single heart tube whereas wild-type embryos show a more differentiated heart consisting of a well-formed AV canal
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• at E8.5 and E9.5, >75% of homozygotes exhibit a disorganized heart that is abnormally positioned in the exocoelomic cavity insted of the putative amniotic cavity
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nervous system
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• at E9.5, homozygotes display an open neural tube with blood cells in the headfold (probably secondary consequences of heart defect)
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growth/size/body
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• at E9.5, homozygotes are smaller than wild-type embryos
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