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Phenotypes Associated with This Genotype
Genotype
MGI:2654203
Allelic
Composition
Trp53bp1tm1Jc/Trp53bp1tm1Jc
Genetic
Background
involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Trp53bp1tm1Jc mutation (1 available); any Trp53bp1 mutation (69 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• in response to 8 Gy of whole body ionizing radiation (IR), all homozygotes die by 14 days from radiation-induced intestinal bleeding and bone marrow failure, whereas most wild-type mice remain viable for at least 2 months after IR treatment
• 9% of homozygotes die at the ages of 1 to 7 months in the absence of overt detectable tumors; possibly due to opportunistic infections

growth/size/body
• homozygotes are significantly smaller than wild-type and heterozygous control mice
• at 5 months of age, both male and female homozygotes weigh significantly less than their wild-type or heterozygous counterparts
• homozygotes of both sexes are growth retarded relative to wild-type or heterozygous control littermates

immune system
• homozygotes show a 40% reduction in thymus cellularity relative to wild-type littermates
• at 6 weeks of age, homozygotes show a maximum 2-fold increase in the percentage of CD4- CD8- progenitors relative to wild-type littermates
• at 6 weeks of age, homozygotes show a ~2-fold reduction in the percentage of CD4+ mature thymocytes and peripheral CD4+ T lymphocytes relative to wild-type littermates

hematopoietic system
• homozygotes show a 40% reduction in thymus cellularity relative to wild-type littermates
• at 6 weeks of age, homozygotes show a maximum 2-fold increase in the percentage of CD4- CD8- progenitors relative to wild-type littermates
• at 6 weeks of age, homozygotes show a ~2-fold reduction in the percentage of CD4+ mature thymocytes and peripheral CD4+ T lymphocytes relative to wild-type littermates

cellular
• mutant MEFs (passage 3) show a tendency toward aneuploidy
• several hrs after exposure to 6 Gy of ionizing radiation (IR), mutant MEFs, like wild-type MEFs, are arrested in G2 but display a delayed exit from the G2/M phase
• at 24 hrs after IR, the % of mitotic cells is ~3-fold lower in nocodazole-treated mutant MEFs than in wild-type cells, as assessed by immunostaining with anti-phospho-histone H3 antibodies
• at 30 min after exposure to 20 Gy of IR, mutant MEFs show a slight defect in intra-S-phase checkpoint regulation relative to wild-type MEFs
• however, mutant MEFs, synchronized by a cycle of serum starvation and release, show a normal G1 arrest in response to 10 to 20 Gy of IR
• mutant ES cells show a 2- to 3-fold increase in ionizing radiation sensitivity relative to wild-type ES cells, revealed by in vitro clonogenic survival assays
• MEFs derived from E14.5 homozygous mutant embryos display a reduced proliferation rate relative to wild-type MEFs
• mutant MEFs (passage 3) show a tendency toward aneuploidy and/or tetraploidy, suggesting a defect in chromosome segregation
• however, no spontaneous chromosomal breaks are detected in mutant MEFs

neoplasm
• homozygotes exhibit an increased incidence of malignant lymphomas, putatively due to an inability to detect or repair abnormal V(D)J recombination
• at 4 to 7 months of age, 8% of homozygotes develop massive thymic lymphomas with or without infiltration of the lymph nodes, spleen, and kidney, not observed in wild-type or heterozygous control mice
• three of these tumors display a CD4+ CD8+ immunophenotype

reproductive system
N
• male homozygotes show no overt defects in spermatogenesis, suggesting normal meiosis
• although both male and female homozygotes are fertile, the average litter size of homozygous mutant intercrosses is slightly reduced relative to that of wild-type intercrosses

homeostasis/metabolism
• in response to 8 Gy of whole body ionizing radiation (IR), all homozygotes die by 14 days from radiation-induced intestinal bleeding and bone marrow failure, whereas most wild-type mice remain viable for at least 2 months after IR treatment

endocrine/exocrine glands
• homozygotes show a 40% reduction in thymus cellularity relative to wild-type littermates
• at 4 to 7 months of age, 8% of homozygotes develop massive thymic lymphomas with or without infiltration of the lymph nodes, spleen, and kidney, not observed in wild-type or heterozygous control mice
• three of these tumors display a CD4+ CD8+ immunophenotype


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/10/2019
MGI 6.14
The Jackson Laboratory