Analysis Tools
hematopoietic system
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• mutants appear normal, are healthy, breed well, and show no hematopoietic abnormalities under normal conditions
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• mutants are phenotypically normal, but multipotent progenitor cells in the bone marrow show a proliferative response to exogenous erythropoietin not seen in wild-type bone marrow, showing an increase in total nucleated cells, myeloid cells and multipotent, clonogenic hematopoietic cells (CFU-S) and CFUs that give rise to granulocytes, erythroid cells, macrophages, and megakaryocytes within the same colony (CFU-GEMM) compared to wild-type
• long term mutant bone marrow culture supplemented with erythropoietin shows prolonged and exponentially increasing hematopoiesis with continued accumulation of nonadherent cells long after controls have ceased proliferation in culture
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