Analysis Tools
limbs/digits/tail
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• mutants display variable AER disruption in the distal margin of the hindlimb consistent with a variable hindlimb phenotype
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• at E10.5, the dorsoventral girth of the AER in the distal margin of the hindlimb is reduced to ~50%
• at E10.5, the dorsoventral girth of the forelimb AER is also reduced; however, the anterior/posterior length of the AER is generally unaffected
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• 22 of 44 mutant mice exhibit mild to severe autopod defects
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• mutant mice display normal forelimbs except in two cases (2/44) where digit 5 was absent or fused to digit 4
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• mutant mice display hindlimb defects of variable penetrance and expressivity, ranging from completely normal to entirely absent hindlimbs
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• 3 of 44 mutant mice show complete absence of hindlimbs
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• 17 of 44 mutant mice show extensive truncations that extend into more proximal segments of the hindlimb
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embryo
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• mutants display extensive apoptosis throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally
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• mutants display variable AER disruption in the distal margin of the hindlimb consistent with a variable hindlimb phenotype
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• at E10.5, the dorsoventral girth of the AER in the distal margin of the hindlimb is reduced to ~50%
• at E10.5, the dorsoventral girth of the forelimb AER is also reduced; however, the anterior/posterior length of the AER is generally unaffected
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cellular
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• mutants display extensive apoptosis throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally
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