Phenotypes Associated with This Genotype

Genotype
MGI:2181732

• Allelic Composition: Lck^tm1Mak/Lck^tm1Mak
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

thymus atrophy ( J:1119 )

• the thymus is sparsely populated with lymphoid cells and has a very thin medulla

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 10-20% of normal (J:37062)

• thymic cellularity is 10-fold less than controls (J:37940)

abnormal thymocyte activation ( J:110847 )

• double positive thymocytes do not efficiently enter into the cell cycle

abnormal lymphocyte morphology ( J:1119 )

• although the overall numbers of cells in the lymph nodes and spleens are normal the B/T cells ratio is inverted with 90 to 95% B cells and 5 to 10% T cells

abnormal T cell morphology ( J:110847 )

• double-positive thymocytes have a slightly lower expression of alpha-beta TCR

abnormal double-negative T cell morphology ( J:110847 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes

decreased T cell number ( J:1119 , J:37062 )

• the number of peripheral T cells is reduced (J:1119)

• alpha-beta T cell numbers are reduced about 10-20 fold compared to controls (J:37062)

decreased thymocyte number ( J:1119 )

• 6x10⁶ to 2x10⁷ cells compared to 2x10⁸ cells in a wild-type thymus

decreased double-positive T cell number ( J:1119 , J:110847 )

• 0.3x10⁷ to 1.6x10⁷ compared to 1.8x10⁸ cells in wild-type mice (J:1119)

• only 12% of thymocytes are double-positive compared to 97% in controls (J:110847)

decreased CD4-positive, alpha-beta T cell number ( J:37940 )

• CD4 T cell numbers are reduced in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:37940 )

• CD8 T cell numbers are reduced in the periphery

decreased gamma-delta T cell number ( J:37062 )

• gamma-delta T cell numbers are reduced about 2-fold compared to controls

decreased gamma-delta intraepithelial T cell number ( J:37940 , J:112977 )

• dendritic epithelial T cell numbers are reduced in the skin (J:37940)

• gamma-delta IEL are absent in these mice (J:112977)

• a CD3^- IEL population believed to represent a precursor population is present in small intestine epithelium (J:112977)

abnormal alpha-beta intraepithelial T cell morphology ( J:112977 )

• alpha-beta IEL are absent in these mice

• a CD3^- IEL population believed to represent a precursor population is present in small intestine epithelium

abnormal single-positive T cell number ( J:37062 )

• very few single-positive thymocytes are detected in these mice and even fewer express mature levels of TCR, CD5 and CD69

decreased IgG1 level ( J:1119 )

increased IgG2a level ( J:1119 )

• marginal

increased IgG3 level ( J:1119 )

• marginal

increased IgM level ( J:1119 )

hematopoietic system

thymus atrophy ( J:1119 )

• the thymus is sparsely populated with lymphoid cells and has a very thin medulla

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 10-20% of normal (J:37062)

• thymic cellularity is 10-fold less than controls (J:37940)

abnormal thymocyte activation ( J:110847 )

• double positive thymocytes do not efficiently enter into the cell cycle

abnormal lymphocyte morphology ( J:1119 )

• although the overall numbers of cells in the lymph nodes and spleens are normal the B/T cells ratio is inverted with 90 to 95% B cells and 5 to 10% T cells

abnormal T cell morphology ( J:110847 )

• double-positive thymocytes have a slightly lower expression of alpha-beta TCR

abnormal double-negative T cell morphology ( J:110847 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes

decreased T cell number ( J:1119 , J:37062 )

• the number of peripheral T cells is reduced (J:1119)

• alpha-beta T cell numbers are reduced about 10-20 fold compared to controls (J:37062)

decreased thymocyte number ( J:1119 )

• 6x10⁶ to 2x10⁷ cells compared to 2x10⁸ cells in a wild-type thymus

decreased double-positive T cell number ( J:1119 , J:110847 )

• 0.3x10⁷ to 1.6x10⁷ compared to 1.8x10⁸ cells in wild-type mice (J:1119)

• only 12% of thymocytes are double-positive compared to 97% in controls (J:110847)

decreased CD4-positive, alpha-beta T cell number ( J:37940 )

• CD4 T cell numbers are reduced in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:37940 )

• CD8 T cell numbers are reduced in the periphery

decreased gamma-delta T cell number ( J:37062 )

• gamma-delta T cell numbers are reduced about 2-fold compared to controls

decreased gamma-delta intraepithelial T cell number ( J:37940 , J:112977 )

• dendritic epithelial T cell numbers are reduced in the skin (J:37940)

• gamma-delta IEL are absent in these mice (J:112977)

• a CD3^- IEL population believed to represent a precursor population is present in small intestine epithelium (J:112977)

abnormal alpha-beta intraepithelial T cell morphology ( J:112977 )

• alpha-beta IEL are absent in these mice

• a CD3^- IEL population believed to represent a precursor population is present in small intestine epithelium

abnormal single-positive T cell number ( J:37062 )

• very few single-positive thymocytes are detected in these mice and even fewer express mature levels of TCR, CD5 and CD69

decreased IgG1 level ( J:1119 )

increased IgG2a level ( J:1119 )

• marginal

increased IgG3 level ( J:1119 )

• marginal

increased IgM level ( J:1119 )

endocrine/exocrine glands

thymus atrophy ( J:1119 )

• the thymus is sparsely populated with lymphoid cells and has a very thin medulla

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 10-20% of normal (J:37062)

• thymic cellularity is 10-fold less than controls (J:37940)

decreased thymocyte number ( J:1119 )

• 6x10⁶ to 2x10⁷ cells compared to 2x10⁸ cells in a wild-type thymus

abnormal thymocyte activation ( J:110847 )

• double positive thymocytes do not efficiently enter into the cell cycle