Analysis Tools
mortality/aging
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• ~38% of mice die within 8 months of birth, compared to 12% of wild-type mice; females often die after giving birth
• despite normal CTL activity toward LCMV infection, and virus clearance, mice die or are moribund within 4 weeks of infection, compared to infected wild-type or Irf1-deficient mice or uninfected controls which remain healthy more than 1 month after infection
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immune system
| N |
• mice have normal numbers of CD8+ T lymphocytes in peripheral blood, lymph node, and spleen
• in response to LCMV infection, mice show normal CTL (cytotoxic T lymphocyte) activity and virus is cleared by 15 days after infection
• humoral response to vesicular stomatitis virus challenge is normal; kinetics of immunoglobulin class switching and antibody levels are comparable to wild-type mice
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• bone marrow contains only 30-70% of wild-type number of mature B cells (B220high/surface IgM (sIgM)-positive cells)
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• in bone marrow, number of Thy-1-positive T cells is reduced to 20-50% of wild-type and heterozygous mice; these are considered to be mature TCR alpha-beta positive T cells
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• some nonspecific cytotoxicity is detected
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• bone marrow cells show a dramatic decrease in colony formation in vitro (70% reduction compared to wild-type cells) in response to LPS, a B cell mitogen; splenocytes show a 30% reduction in colony formation in response to LPS
• reduced colony formation (by 40%) by bone marrow cells is seen in response to Il3, Il7 and CSF1 indicating that responses of multipotential progenitors, pre-B cells and macrophages in bone marrow are altered
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• serum IgG2a levels are significantly reduced
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• despite normal CTL activity toward LCMV infection, and virus clearance, mice die or are moribund within 4 weeks of infection, compared to infected wild-type or Irf1-deficient mice or uninfected controls which remain healthy more than 1 month after infection
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hematopoietic system
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• bone marrow cells show a dramatic decrease in colony formation in vitro (70% reduction compared to wild-type cells) in response to LPS, a B cell mitogen; splenocytes show a 30% reduction in colony formation in response to LPS
• reduced colony formation (by 40%) by bone marrow cells is seen in response to Il3, Il7 and CSF1 indicating that responses of multipotential progenitors, pre-B cells and macrophages in bone marrow are altered
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• bone marrow contains only 30-70% of wild-type number of mature B cells (B220high/surface IgM (sIgM)-positive cells)
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• in bone marrow, number of Thy-1-positive T cells is reduced to 20-50% of wild-type and heterozygous mice; these are considered to be mature TCR alpha-beta positive T cells
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• serum IgG2a levels are significantly reduced
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integument
skin lesions
(
J:64286
)
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• older animals exhibit erosions and ulcers around the neck, back and abdomen; in severe cases, patchy scars and postinflammatory hair loss can result
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cellular
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• bone marrow cells show a dramatic decrease in colony formation in vitro (70% reduction compared to wild-type cells) in response to LPS, a B cell mitogen; splenocytes show a 30% reduction in colony formation in response to LPS
• reduced colony formation (by 40%) by bone marrow cells is seen in response to Il3, Il7 and CSF1 indicating that responses of multipotential progenitors, pre-B cells and macrophages in bone marrow are altered
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