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Phenotypes Associated with This Genotype
Genotype
MGI:2176346
Allelic
Composition
Mpotm1Mae/Mpotm1Mae
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mpotm1Mae mutation (0 available); any Mpo mutation (54 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• as expected, PMA-stimulated peritoneal exudate neutrophils from homozygotes fail to generate hypochlorous acid (HOCl) from hydrogen peroxide, due to absence of peroxidase activity
• also, peritoneal exudate neutrophils from homozygotes show a slightly higher level of O2- generation than those of wild-type mice
• homozygotes are viable, fertile and developmentally normal with normal clearance of intraperitoneal S. aureus by 48 hrs post-infection
• however, homozygotes show increased susceptibility to intratracheal infection with C. albicans, with severe alveolar and peribronchiolar pneumonia resulting in death of two-thirds of mutants by 5 days post-infection even at a relatively low dose (4 106 fungi/mouse)
• when challenged intraperitoneally at a similar dose (4 106 fungi/mouse), both wild-type and mutant mice show no obvious signs of severe infection at 7 days after challenge, despite significant fungal dissemination in various mutant organs, with lungs and kidneys being most severely affected
• at a higher i.p. dose (108 fungi/mouse), all homozygotes die by 2 days, whereas all wild-type mice survive for 7 days without many signs of distress

homeostasis/metabolism
• as expected, PMA-stimulated peritoneal exudate neutrophils from homozygotes fail to generate hypochlorous acid (HOCl) from hydrogen peroxide, due to absence of peroxidase activity
• also, peritoneal exudate neutrophils from homozygotes show a slightly higher level of O2- generation than those of wild-type mice

hematopoietic system
• as expected, PMA-stimulated peritoneal exudate neutrophils from homozygotes fail to generate hypochlorous acid (HOCl) from hydrogen peroxide, due to absence of peroxidase activity
• also, peritoneal exudate neutrophils from homozygotes show a slightly higher level of O2- generation than those of wild-type mice


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory