Mouse Genome Informatics
ht
    CrebbpGt(U-San)112Imeg/Crebbp+
involves: C57BL/6 * CBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
       
mortality/aging
• a subset of F2 heterozygous pups die within several days after birth

growth/size/body
• at death, heterozygotes fed a high-carbohydrate (HC) diet display a significantly reduced fat mass relative to similarly fed wild-type mice
• however, at P3, heterozygotes contain the same amount of BAT and WAT as wild-type mice
• at 2-14 weeks, heterozygotes show a 30% reduction in body weight relative to wild-type mice
• however, heterozygotes gain weight at a rate that is comparable to that observed in wild-type mice
• in contrast to wild-type mice, heterozygotes fail to show a time-dependent increase in weight on a high-fat (HF) diet
• heterozygotes appear to be protected against HF diet-induced triglyceride accumulation in WAT
• heterozygotes exhibit significant intrauterine growth retardation in both weight and height relative to wild-type embryos

craniofacial
• at 19 dpc, heterozygotes exhibit a large anterior fontanel
• heterozygotes display widening of the frontal bone

skeleton
• at 19 dpc, heterozygotes exhibit a large anterior fontanel
• heterozygotes display widening of the frontal bone
• 1 of 30 heterozygotes display severe scoliosis
• heterozygotes exhibit delayed osseous maturation; notably, neither broad thumbs nor broad halluces are observed

cardiovascular system
• at 19.5 dpc, 9 of 54 heterozygous fetuses (16.6%) display non-overlapping cardiac abnormalities
• at 19.5 dpc, 1 of 54 heterozygous fetuses exhibit a bicuspid valve
• at 19.5 dpc, 1 of 54 heterozygous fetuses exhibit an atrial septal defect
• at 19.5 dpc, 7 of 54 heterozygous fetuses exhibit a ventricular septal defect of the membranous type

behavior/neurological
• heterozygotes display impaired long-term memory (LTM) in a step-through-type passive avoidance test and a cued fear-conditioning test
• in contrast, heterozygotes exhibit normal short-term memory in a Y-maze test as well as normal spatial learning in a water maze test
• hypolocomotion in a dark environment is noted throughout the entire 60 min observation period in the vertical activity, but only in the first 5 min in the horizontal activity
• heterozygotes display hypolocomotion in a dark environment but not in a light environment
• at least 2 of 54 heterozygotes display seizures

nervous system
N
• heterozygotes exhibit a normal brain morphology: despite the LTM deficit, neither sensorimotor nor gross neuroanatomical anomalies are observed (J:53370)
• at least 2 of 54 heterozygotes display seizures

limbs/digits/tail
• 1 of 30 heterozygotes exhibit oligodactyly

adipose tissue
• at death, heterozygotes fed a high-carbohydrate (HC) diet display a significantly reduced fat mass relative to similarly fed wild-type mice
• however, at P3, heterozygotes contain the same amount of BAT and WAT as wild-type mice
• at 8 months, heterozygotes show a significant reduction in WAT weight per body weight; the weight of other tissues, including brown adipose tissue, remains unchanged
• reduced WAT mass is attributed to the inhibition of triglyceride accumulation in WAT
• at 8 months, heterozygotes fed a HC diet show a marked reduction in adipocyte size relative to similarly fed wild-type mice

cellular
in vitro, embryonic fibroblasts from heterozygous mice exhibit a reduced ability to differentiate into adipocytes upon induction with conventional hormonal stimuli

homeostasis/metabolism
• in contrast to wild-type mice, heterozygotes fail to show a time-dependent increase in weight on a high-fat (HF) diet
• heterozygotes appear to be protected against HF diet-induced triglyceride accumulation in WAT
• heterozygotes show higher plasma insulin levels during glucose tolerance tests suggesting increased insulin secretion; however, this rise in insulin levels does not reach statistical significance
• heterozygotes fed a HF diet display increased serum leptin levels relative to the severely reduced WAT mass
• in addition, heterozygotes fed a HF diet exhibit increased leptin sensitivity in response to exogenous leptin
• heterozygotes fed a HF diet display reduced serum levels of free fatty acids relative to wild-type mice
• heterozygotes exhibit a marked increase in resting oxygen consumption relative to wild-type mice
• in contrast, food intake remains relatively unchanged on either the HC or HF diet
• heterozygotes display increased glucose tolerance relative to wild-type mice on both a HC and a HF diet
• despite lipodystrophy, heterozygotes exhibit an enhanced glucose-lowering insulin effect relative to wild-type mice
• heterozygotes fed a HF diet show a significant increase in serum adiponectin levels, despite their markedly reduced WAT mass
• heterozygotes display changes in gene expression associated with decreased tissue triglyceride content in skeletal muscle and liver
• heterozygotes fed a HF diet display reduced Tnfa mRNA levels in WAT relative to wild-type mice

immune system
• heterozygotes fed a HF diet display reduced Tnfa mRNA levels in WAT relative to wild-type mice

Mouse Models of Human Disease
OMIM IDRef(s)
Rubinstein-Taybi Syndrome 1; RSTS1 180849 J:53370