Phenotypes for Tnf MGI:2175017 MGI Mouse

decreased spleen B cell follicle number ( J:95684 )

• absence of polarized B-cell follicles

absent spleen germinal center ( J:41992 , J:80616 )

• mutants fail to develop germinal centers in the spleen following immunization with SRBC (J:41992)

decreased spleen white pulp amount ( J:80616 )

• gradual reduction of white pulp size

decreased IgG level ( J:41992 )

• mutants exhibit a deficiency in maturation of normal antibody response to T-cell dependent antigen (SRBC), showing depressed IgG titers but normal IgM titers

decreased circulating tumor necrosis factor level ( J:95684 )

• serum TNF levels are reduced in mutants injected with LPS and can not be detected on day 2 of Listeria monocytogenes infection

abnormal follicular dendritic cell physiology ( J:41992 )

• the normal uptake of aggregated gamma globulin by follicular dendritic cells in the spleen of immunized mutants is absent

abnormal cytokine secretion ( J:41992 )

• production of colony-stimulating factor (CSF), specifically granulocyte-macrophage (GM)-CSF, is reduced after i.p. injection of lipopolysaccharide (LPS)

abnormal chemokine secretion ( J:95684 )

• induction of MCP-1 is reduced in spleens of mutants infected with Listeria monocytogenes

decreased susceptibility to autoimmune disorder ( J:95684 )

• increased resistance to liver injury after ConA-induced autoimmune hepatitis, with only infiltrating cells but no liver necrosis detected

decreased susceptibility to endotoxin shock ( J:41992 , J:95684 )

• mutants are resistant to the lethality of minute doses of lipopolysaccharide (LPS) following D-galactosamine treatment, however at higher concentrations (1200ug), they succumb to illness to the same extent as wild-type (J:41992)

• mutants are resistant to staphylococcal enterotoxin B in the presence of D-gal (J:41992)

• protected from S. aureus enterotoxin B induced toxic shock (J:95684)

abnormal susceptibility to infection ( J:41992 )

increased susceptibility to bacterial infection ( J:41992 , J:80616 , J:95684 )

• mutants show a delayed (40 days post injection instead of the 10-14 seen in wild-type), but progressive, response to heat-killed Corynebacterium parvum, that, unlike in wild-type, eventually leads to death by 80 days (J:41992)

• increased susceptibility to Salmonella enterica infection (J:80616)

• increased susceptibility to Listeria monocytogenes infections, with mutants dying by day 8 while wild-type mice survive (J:80616)

• loss of resistance against Listeria monocytogenes (J:95684)

increased susceptibility to fungal infection ( J:41992 )

• mutants exhibit increased susceptibility to Candida albicans challenge; 100% die by day 7 post-infection