Analysis Tools
mortality/aging
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• embryos die before E7.5
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embryo
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• in vivo and in vitro analysis of cell growth in mutant embryos show that the proliferative capacity of mutant embryos is impaired by E6.5
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• at E6.5, less severely affected embryos are half the normal size
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• at E6.5, mutant embryos are morphologically abnormal
• more severely affected embryos resemble egg cylinders
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• expression analysis of brachyury, a marker of embryonic mesoderm, confirmed that mutant embryos fail to make mesoderm
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• at E6.5, less severely affected embryos show epiblast, no primitive streak and a proamniotic cavity
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• at E6.5, no clear boundary between the embryonic and extraembryonic tissues is apparent
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• at E6.5, less severely affected embryos show a very thin parietal endoderm
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• at E6.5, the extraembryonic region appears disorganized with no compact layer of trophoblast cells and an abundance of giant cells
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• Mash2 expression analysis indicates that no diploid trophoblast cells are present in the mutant extraembryonic region
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• at E6.5, the extraembryonic region appears disorganized
• the most severely affected embryos exhibit no detectable extraembryonic region
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• at E6.5, less severely affected embryos show a very thin visceral endoderm
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growth/size/body
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• in vivo and in vitro analysis of cell growth in mutant embryos show that the proliferative capacity of mutant embryos is impaired by E6.5
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• at E6.5, less severely affected embryos are half the normal size
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