Phenotypes associated with this allele
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sox7tm1.1Dsco mutation
(0 available);
any
Sox7 mutation
(21 available)
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mortality/aging
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• almost all die between E10.5 and E14.5, rare survivors are found beyond E14.5
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embryo
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• detected in many (22 of 28) embryos at E10.5
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• many (22 of 28) display failure of yolk sac remodeling at E10.5
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homeostasis/metabolism
cardiovascular system
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• collagen gel atrioventricular canal explants from E9.5 embryos showed significantly lower numbers of migrating mesenchymal cells than wild-type explants, suggesting a decrease in endocardial-to-mesenchymal transition (EndMT)
• RNA-seq analysis of E9.5 heart tubes showed alterations in genes involved in epithelial-to-mesenchymal transition with marked downregulation of Wnt4 and, to a lesser extent, Bmp2
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• at E9.5, AV canals show significantly decreased Wnt4 transcript levels in the endocardium as well as reduced Bmp2 transcript levels in the myocardium
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• at E9.5/E10.5, atrioventricular (AV) endocardial cushions are hypocellular with a severe reduction in mesenchymal cell density relative to wild-type controls
• however, separation of the endocardium from the myocardium and the size of AV cushions are normal at E10.5
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• in many (17 of 28) mice at E10.5
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growth/size/body
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• detected in many (22 of 28) embryos at E10.5
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sox7tm1.1Dsco mutation
(0 available);
any
Sox7 mutation
(21 available)
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muscle
N |
• Background Sensitivity: unlike in mice on a mixed 129 and C57BL/6 background, mice backcrossed through 8 generations onto a C57BL6 background do not display congenital diaphragmatic hernia
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Allelic Composition |
Sox7tm1.1Dsco/Sox7+
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Genetic Background |
involves: 129S/SvEv * C57BL/6 |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sox7tm1.1Dsco mutation
(0 available);
any
Sox7 mutation
(21 available)
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muscle
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• in some cases the gallbladder is fused to the underside of the anterior diaphragm
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• Background Sensitivity: around 14% of mice (10 of 71, age P28 to adult) on a mixed 129 and C57BL/6 background have congenital diaphragmatic hernia located directly behind the sternum in the ventral midline; hernias are not seen in mice on a congenic C57BL/6 background
• in severe cases the gallbladder and a pedunculated portion of the liver are found in the thoracic cavity
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liver/biliary system
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• in some cases the gallbladder is fused to the underside of the anterior diaphragm
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endocrine/exocrine glands
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• in some cases the gallbladder is fused to the underside of the anterior diaphragm
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Allelic Composition |
Sox7tm1.1Dsco/Sox7+
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Genetic Background |
involves: 129/Sv * 129S/SvEv * C57BL/6 * C57BL/6J |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sox7tm1.1Dsco mutation
(0 available);
any
Sox7 mutation
(21 available)
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mortality/aging
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• although present in normal ratios at E15.5/E16.5, heterozygotes are recovered at lower than Mendelian ratios at weaning; however, increase in lethality between E15.5/E16.5 and weaning does not reach statistical significance
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cardiovascular system
N |
• atrioventricular (AV) endocardial cushions show no significant alterations in mesenchymal cell density at E10.5; no ventricular septal defects are identified at E15.5
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sox7tm1.1Dsco mutation
(0 available);
any
Sox7 mutation
(21 available)
Sox7tm1Dsco mutation
(0 available);
any
Sox7 mutation
(21 available)
Tg(Tek-cre)1Ywa mutation
(6 available)
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mortality/aging
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• most embryos die prior to E15.5; two rare survivors are found at E15.5
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cardiovascular system
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• one of 2 rare embryos that survived to E15.5 show a ventricular septal defect (VSD), not observed in control embryos
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sox7tm1.1Dsco mutation
(0 available);
any
Sox7 mutation
(21 available)
Wnt4tm2(EGFP/cre/ERT2)Amc mutation
(1 available);
any
Wnt4 mutation
(20 available)
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mortality/aging
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• although present in normal ratios at E15.5/E16.5, double heterozygotes are recovered at lower than Mendelian ratios at weaning; however, increase in lethality between E15.5/E16.5 and weaning does not reach statistical significance
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cardiovascular system
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• at E10.5, atrioventricular (AV) endocardial cushions are hypocellular with a significant reduction in mesenchymal cell density relative to wild-type and single heterozygous controls
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• at E15.5, three of 8 (38%) of double heterozygotes develop VSDs, not observed in wild-type or single heterozygous controls
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• at E15.5, two of 8 double heterozygotes develop perimembranous VSDs
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• at E15.5, one of 8 double heterozygotes shows a muscular VSD
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