Phenotypes associated with this allele

• Allele Symbol: Rras^tm1Itl
• Allele Name: targeted mutation 1, inGenious Targeting Laboratory
• Allele ID: MGI:5314105
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rras^tm1Itl/Rras^tm1Itl	either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * C57BL/6)	MGI:5314108
hm2	Rras^tm1Itl/Rras^tm1Itl	involves: 129S6/SvEvTac * C57BL/6	MGI:5314109
ht3	Rras^tm1Itl/Rras^+	involves: 129S6/SvEvTac * C57BL/6	MGI:5314110

Genotype
MGI:5314108

hm1

• Allelic Composition: Rras^tm1Itl/Rras^tm1Itl
• Genetic Background: either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:181730 )

N • mice exhibit normal T cell development in the thymus and in CD11c+, B220+, CD8+, or CD4+ cells in the spleen

Genotype
MGI:5314109

hm2

• Allelic Composition: Rras^tm1Itl/Rras^tm1Itl
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

immune system

abnormal dendritic cell morphology ( J:181730 )

• LPS-induced dendritic cell maturation is impaired compared with wild-type cells

abnormal dendritic cell physiology ( J:181730 )

• when splenic antibody-presenting cells are co-cultured with allogenic T cell, proliferation of T cells is reduced compared to when wild-type cells are used

• LPS-activated splenic dendritic cells stimulates less T cell proliferation than wild-type cells

• splenic dendritic cells from mice injected with LPS of ovalbumin are less effective in priming a T cell response compared with cells from wild-type mice

• dendritic cells exhibit impaired cell spreading and T cell synapse formation compared with wild-type cells

neoplasm

increased tumor growth/size ( J:181730 )

• mice injected with B16-F10 mouse melanoma cells exhibit increased tumor incidence (100% versus 14% by 2 weeks) and increased tumor volume (5-fold by 3 weeks) compared with wild-type mice

hematopoietic system

abnormal dendritic cell morphology ( J:181730 )

• LPS-induced dendritic cell maturation is impaired compared with wild-type cells

Genotype
MGI:5314110

ht3

• Allelic Composition: Rras^tm1Itl/Rras⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

neoplasm

increased tumor growth/size ( J:181730 )

• mice injected with B16-F10 mouse melanoma cells exhibit increased tumor incidence (78% versus 14% by 2 weeks) and increased tumor volume (2-fold by 3 weeks) compared with wild-type mice