Phenotypes associated with this allele

• Allele Symbol: Rnf128^tm1.1Flv
• Allele Name: targeted mutation 1.1, Richard Flavell
• Allele ID: MGI:4365554
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rnf128^tm1.1Flv/Rnf128^tm1.1Flv	involves: 129S6/SvEvTac * C57BL/6	MGI:4365569
cx2	Rnf128^tm1.1Flv/Rnf128^tm1.1Flv Tg(TcraTcrb)425Cbn/0	involves: 129S6/SvEvTac * BALB/c * C57BL/6	MGI:4365571
cx3	Rnf128^tm1.1Flv/Y Tg(TcraTcrb)425Cbn/0	involves: 129S6/SvEvTac * BALB/c * C57BL/6	MGI:4365572
ot4	Rnf128^tm1.1Flv/Y	involves: 129S6/SvEvTac * C57BL/6	MGI:4365570

Genotype
MGI:4365569

hm1

• Allelic Composition: Rnf128^tm1.1Flv/Rnf128^tm1.1Flv
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased T cell proliferation ( J:153216 )

♀ • anti-CD3 antibody-stimulated CD4+ T cells exhibit increased proliferation compared with similarly treated wild-type cells

♀ • following ionomycin-induced anergy, T cell proliferation is increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

♀ • T cells are hyperresponsive to TCR stimulation alone or with anti-CD28 compared with similarly treated wild-type T cells

♀ • proliferation of stimulated helper T cells is increased compared with similarly treated wild-type cells

abnormal CD4-positive, alpha-beta T cell physiology ( J:153216 )

♀ • after stimulation, T cell death after 2 days is decreased while it is increased after 5 days compared with similarly treated wild-type cells

♀ • viability of stimulated helper T cells is reduced 50% compared to in similarly treated wild-type cells

increased circulating interleukin-2 level ( J:153216 )

♀ • following ionomycin-induced anergy, IL2 plasma levels are increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

increased interferon-gamma secretion ( J:153216 )

♀ • stimulated helper T cells over-produce IFN-gamma compared with similarly treated wild-type cells

abnormal peripheral T cell anergy ( J:153216 )

♀ • splenic CD4+ T cells exhibit defective ionomycin-induced anergy with increased proliferation and IL2 levels compared with similarly treated wild-type cells

♀ • mice cannot be orally tolerized against experimental allergic encephalitis unlike similarly treated wild-type mice

homeostasis/metabolism

increased circulating interleukin-2 level ( J:153216 )

♀ • following ionomycin-induced anergy, IL2 plasma levels are increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

hematopoietic system

increased T cell proliferation ( J:153216 )

♀ • anti-CD3 antibody-stimulated CD4+ T cells exhibit increased proliferation compared with similarly treated wild-type cells

♀ • following ionomycin-induced anergy, T cell proliferation is increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

♀ • T cells are hyperresponsive to TCR stimulation alone or with anti-CD28 compared with similarly treated wild-type T cells

♀ • proliferation of stimulated helper T cells is increased compared with similarly treated wild-type cells

abnormal CD4-positive, alpha-beta T cell physiology ( J:153216 )

♀ • after stimulation, T cell death after 2 days is decreased while it is increased after 5 days compared with similarly treated wild-type cells

♀ • viability of stimulated helper T cells is reduced 50% compared to in similarly treated wild-type cells

cellular

increased T cell proliferation ( J:153216 )

♀ • anti-CD3 antibody-stimulated CD4+ T cells exhibit increased proliferation compared with similarly treated wild-type cells

♀ • following ionomycin-induced anergy, T cell proliferation is increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

♀ • T cells are hyperresponsive to TCR stimulation alone or with anti-CD28 compared with similarly treated wild-type T cells

♀ • proliferation of stimulated helper T cells is increased compared with similarly treated wild-type cells

Genotype
MGI:4365571

cx2

• Allelic Composition: Rnf128^tm1.1Flv/Rnf128^tm1.1Flv; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129S6/SvEvTac * BALB/c * C57BL/6

immune system

decreased T cell proliferation ( J:153216 )

♀

increased circulating interleukin-2 level ( J:153216 )

♀

abnormal peripheral T cell anergy ( J:153216 )

♀ • despite increasing antigen doses, CD4+ T cells cannot be tolerized unlike similarly treated wild-type cells and continue to exhibit increased T cell proliferation and IL2 production

homeostasis/metabolism

increased circulating interleukin-2 level ( J:153216 )

♀

hematopoietic system

decreased T cell proliferation ( J:153216 )

♀

cellular

decreased T cell proliferation ( J:153216 )

♀

Genotype
MGI:4365572

cx3

• Allelic Composition: Rnf128^tm1.1Flv/Y; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129S6/SvEvTac * BALB/c * C57BL/6

immune system

decreased T cell proliferation ( J:153216 )

♂

increased circulating interleukin-2 level ( J:153216 )

♂

abnormal peripheral T cell anergy ( J:153216 )

♂ • despite increasing antigen doses, CD4+ T cells cannot be tolerized unlike similarly treated wild-type cells and continue to exhibit increased T cell proliferation and IL2 production

homeostasis/metabolism

increased circulating interleukin-2 level ( J:153216 )

♂

hematopoietic system

decreased T cell proliferation ( J:153216 )

♂

cellular

decreased T cell proliferation ( J:153216 )

♂

Genotype
MGI:4365570

ot4

• Allelic Composition: Rnf128^tm1.1Flv/Y
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

immune system

increased T cell proliferation ( J:153216 )

♂ • anti-CD3 antibody-stimulated CD4+ T cells exhibit increased proliferation compared with similarly treated wild-type cells

♂ • following ionomycin-induced anergy, T cell proliferation is increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

♂ • T cells are hyperresponsive to TCR stimulation alone or with anti-CD28 compared with similarly treated wild-type T cells

♂ • proliferation of stimulated helper T cells is increased compared with similarly treated wild-type cells

abnormal CD4-positive, alpha-beta T cell physiology ( J:153216 )

♂ • after stimulation, T cell death after 2 days is decreased while it is increased after 5 days compared with similarly treated wild-type cells

♂ • viability of stimulated helper T cells is reduced 50% compared to in similarly treated wild-type cells

increased circulating interleukin-2 level ( J:153216 )

♂ • following ionomycin-induced anergy, IL2 plasma levels are increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

increased interferon-gamma secretion ( J:153216 )

♂ • stimulated helper T cells over-produce IFN-gamma compared with similarly treated wild-type cells

abnormal peripheral T cell anergy ( J:153216 )

♂ • splenic CD4+ T cells exhibit defective ionomycin-induced anergy with increased proliferation and IL2 levels compared with similarly treated wild-type cells

♂ • mice cannot be orally tolerized against experimental allergic encephalitis unlike similarly treated wild-type mice

homeostasis/metabolism

increased circulating interleukin-2 level ( J:153216 )

♂ • following ionomycin-induced anergy, IL2 plasma levels are increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

hematopoietic system

increased T cell proliferation ( J:153216 )

♂ • anti-CD3 antibody-stimulated CD4+ T cells exhibit increased proliferation compared with similarly treated wild-type cells

♂ • following ionomycin-induced anergy, T cell proliferation is increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

♂ • T cells are hyperresponsive to TCR stimulation alone or with anti-CD28 compared with similarly treated wild-type T cells

♂ • proliferation of stimulated helper T cells is increased compared with similarly treated wild-type cells

abnormal CD4-positive, alpha-beta T cell physiology ( J:153216 )

♂ • after stimulation, T cell death after 2 days is decreased while it is increased after 5 days compared with similarly treated wild-type cells

♂ • viability of stimulated helper T cells is reduced 50% compared to in similarly treated wild-type cells

cellular

increased T cell proliferation ( J:153216 )

♂ • anti-CD3 antibody-stimulated CD4+ T cells exhibit increased proliferation compared with similarly treated wild-type cells

♂ • following ionomycin-induced anergy, T cell proliferation is increased compared to in wild-type mice and fails to exhibit suppression when CD4+ T cells are co-cultured with wild-type T regulatory cells expressing Foxp3^tm1Flv

♂ • T cells are hyperresponsive to TCR stimulation alone or with anti-CD28 compared with similarly treated wild-type T cells

♂ • proliferation of stimulated helper T cells is increased compared with similarly treated wild-type cells