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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Mcl1tm1Yen
targeted mutation 1, Hsin-Fang Yang-Yen
MGI:3837368
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Mcl1tm1Yen/Mcl1tm1Yen B6.129-Mcl1tm1Yen MGI:3837391
cx2
 		Mcl1tm1Yen/Mcl1tm1Yen
Tg(TcraTcrb)425Cbn/? 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3837392
cx3
 		Mcl1tm1Yen/Mcl1tm1Yen
Tg(TcraH-Y,TcrbH-Y)71Vbo/? 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2 MGI:3837393


Genotype
MGI:3837391
hm1
Allelic
Composition		 Mcl1tm1Yen/Mcl1tm1Yen
Genetic
Background		 B6.129-Mcl1tm1Yen
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mcl1tm1Yen mutation (0 available); any Mcl1 mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased double-negative T cell number ( J:146246 )
• absolute numbers for both DN1 and DN2 populations are slightly but significantly reduced in the thymus
abnormal double-positive T cell morphology ( J:146246 )
• the proportion of double positive thymocytes undergoing positive selection (i.e. expressing high levels of activation markers) is reduced
• double-positive thymocytes have higher rates of apoptosis when cultured without growth factors or when stimulated in vivo by anti-CD3 treatment
decreased CD8-positive, alpha-beta T cell number ( J:146246 )
• mice have a 35% reduction in CD8+ T cells in the spleen and lymph nodes
decreased single-positive T cell number ( J:146246 )
• single-positive CD4 and CD8 T cells in the thymus have slight reduction in numbers within the thymus
abnormal CD8-positive, alpha-beta T cell physiology ( J:146246 )
• 50% more CD8+ T cells are apoptotic than controls when cultured 36 hours without growth factors
• the in vivo viability of CD8+ T cells is also 50% of controls 5 days after transfer to immunocompetent hosts

hematopoietic system
decreased double-negative T cell number ( J:146246 )
• absolute numbers for both DN1 and DN2 populations are slightly but significantly reduced in the thymus
abnormal double-positive T cell morphology ( J:146246 )
• the proportion of double positive thymocytes undergoing positive selection (i.e. expressing high levels of activation markers) is reduced
• double-positive thymocytes have higher rates of apoptosis when cultured without growth factors or when stimulated in vivo by anti-CD3 treatment
decreased CD8-positive, alpha-beta T cell number ( J:146246 )
• mice have a 35% reduction in CD8+ T cells in the spleen and lymph nodes
decreased single-positive T cell number ( J:146246 )
• single-positive CD4 and CD8 T cells in the thymus have slight reduction in numbers within the thymus
abnormal CD8-positive, alpha-beta T cell physiology ( J:146246 )
• 50% more CD8+ T cells are apoptotic than controls when cultured 36 hours without growth factors
• the in vivo viability of CD8+ T cells is also 50% of controls 5 days after transfer to immunocompetent hosts




Genotype
MGI:3837392
cx2
Allelic
Composition		 Mcl1tm1Yen/Mcl1tm1Yen
Tg(TcraTcrb)425Cbn/?
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mcl1tm1Yen mutation (0 available); any Mcl1 mutation (33 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal double-positive T cell morphology ( J:146246 )
• mice have decreased numbers of double positive thymocytes expressing the transgenic TCR
decreased CD4-positive, alpha-beta T cell number ( J:146246 )
• mice have decreased frequency and numbers of transgenic CD4+ T cells in the thymus and in the periphery

hematopoietic system
abnormal double-positive T cell morphology ( J:146246 )
• mice have decreased numbers of double positive thymocytes expressing the transgenic TCR
decreased CD4-positive, alpha-beta T cell number ( J:146246 )
• mice have decreased frequency and numbers of transgenic CD4+ T cells in the thymus and in the periphery




Genotype
MGI:3837393
cx3
Allelic
Composition		 Mcl1tm1Yen/Mcl1tm1Yen
Tg(TcraH-Y,TcrbH-Y)71Vbo/?
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mcl1tm1Yen mutation (0 available); any Mcl1 mutation (33 available)
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal double-positive T cell morphology ( J:146246 )
• mice have decreased numbers of double positive thymocytes expressing the transgenic TCR
decreased CD8-positive, alpha-beta T cell number ( J:146246 )
• female mice have decreased frequency and numbers of transgenic CD8+ T cells in the thymus and in the periphery

hematopoietic system
abnormal double-positive T cell morphology ( J:146246 )
• mice have decreased numbers of double positive thymocytes expressing the transgenic TCR
decreased CD8-positive, alpha-beta T cell number ( J:146246 )
• female mice have decreased frequency and numbers of transgenic CD8+ T cells in the thymus and in the periphery




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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory