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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Orai1tm1Rao
targeted mutation 1, Anjana Rao
MGI:3810487
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Orai1tm1Rao/Orai1tm1Rao C57BL/6-Orai1tm1Rao MGI:3810507
hm2
Orai1tm1Rao/Orai1tm1Rao involves: C57BL/6NTac * ICR MGI:3810509
ht3
Orai1tm1Rao/Orai1+ C57BL/6-Orai1tm1Rao MGI:3810508


Genotype
MGI:3810507
hm1
Allelic
Composition
Orai1tm1Rao/Orai1tm1Rao
Genetic
Background
C57BL/6-Orai1tm1Rao
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Orai1tm1Rao mutation (1 available); any Orai1 mutation (27 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• all pups die between 0.5 and 1.5 days after birth
• null mice are born at lower than the expected Mendelian frequency

cellular
• MEFs derived from these mice have defects in store-operated Ca2+ influx




Genotype
MGI:3810509
hm2
Allelic
Composition
Orai1tm1Rao/Orai1tm1Rao
Genetic
Background
involves: C57BL/6NTac * ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Orai1tm1Rao mutation (1 available); any Orai1 mutation (27 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• only a third of the mice live past 90 days of age
• half the mice die within one day of birth

growth/size/body
• mice are consistently 25-30% smaller by bodyweight compared to controls regardless of age

immune system
• mast cells show defects in store-operated Ca2+ influx when activated by thapsigargin treatment
• B cells show defects in store-operated Ca2+ influx when activated through their BCR, by thapsigargin treatment, or my maximally stimulating them with ionomycin
• nave T cells show defects in store-operated Ca2+ influx only when activated in the presence of low (0.5mM) extra-cellular Ca2+
• antigen experienced T cells show defects in store-operated Ca2+ influx at physiological extra-cellular calcium concentrations
• double-positive thymocytes show a substantial decrease in Ca2+ flux when activated
• there is a substantial decrease in the number of IFN-gamma producing CD4+ T cells, as well as a reduction in mean cytokine production per cell, when cultured in vitro under nave and Th1-differentiating conditions
• cytolytic CD8+ T cells also have decreased IFN-gamma production
• there is a substantial decrease in the number of IL10-producing CD4+ T cells, as well as a reduction in mean cytokine production per cell, when cultured in vitro under Th2-differentiating conditions
• there is a substantial decrease in the number of IL2-producing CD4+ T cells, as well as a reduction in mean cytokine production per cell, when cultured in vitro under nave and Th1-differentiating conditions
• there is a substantial decrease in the number of IL4-producing CD4+ T cells, as well as a reduction in mean cytokine production per cell, when cultured in vitro under Th2-differentiating conditions
• eyelid irritation is observed in all mice with variable severity
• irritation is independent of cage bedding (i.e. not related to allergies)

vision/eye
• eyelid irritation is observed in all mice with variable severity
• irritation is independent of cage bedding (i.e. not related to allergies)

hematopoietic system
• mast cells show defects in store-operated Ca2+ influx when activated by thapsigargin treatment
• B cells show defects in store-operated Ca2+ influx when activated through their BCR, by thapsigargin treatment, or my maximally stimulating them with ionomycin
• nave T cells show defects in store-operated Ca2+ influx only when activated in the presence of low (0.5mM) extra-cellular Ca2+
• antigen experienced T cells show defects in store-operated Ca2+ influx at physiological extra-cellular calcium concentrations
• double-positive thymocytes show a substantial decrease in Ca2+ flux when activated

integument
• sporadic hair loss occurs in these mice
• mice showed a patchy pattern of hair loss, concentrated on the occipital region of the skin of the skull
• affected mouse in initially show a progressive spread of the hair-less region toward the tail, and then a partial regrowth of hair in the affected region
• with increasing age, hairless patches are replaced with sparse hair growth
• vibrissae are shorter and thinner than controls
• vibrissae follicles are smaller and less developed
• mice have thinner skin, with fewer but more-elongated keratinocytes

endocrine/exocrine glands
• double-positive thymocytes show a substantial decrease in Ca2+ flux when activated




Genotype
MGI:3810508
ht3
Allelic
Composition
Orai1tm1Rao/Orai1+
Genetic
Background
C57BL/6-Orai1tm1Rao
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Orai1tm1Rao mutation (1 available); any Orai1 mutation (27 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• B cells show defects in store-operated Ca2+ influx when activated
• CD4+ T cells show defects in store-operated Ca2+ influx when activated

hematopoietic system
• B cells show defects in store-operated Ca2+ influx when activated
• CD4+ T cells show defects in store-operated Ca2+ influx when activated





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory