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Allele Symbol Allele Name Allele ID |
Orai1tm1Rao targeted mutation 1, Anjana Rao MGI:3810487 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• all pups die between 0.5 and 1.5 days after birth
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• null mice are born at lower than the expected Mendelian frequency
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• MEFs derived from these mice have defects in store-operated Ca2+ influx
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• only a third of the mice live past 90 days of age
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• half the mice die within one day of birth
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• mice are consistently 25-30% smaller by bodyweight compared to controls regardless of age
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• mast cells show defects in store-operated Ca2+ influx when activated by thapsigargin treatment
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• B cells show defects in store-operated Ca2+ influx when activated through their BCR, by thapsigargin treatment, or my maximally stimulating them with ionomycin
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• nave T cells show defects in store-operated Ca2+ influx only when activated in the presence of low (0.5mM) extra-cellular Ca2+
• antigen experienced T cells show defects in store-operated Ca2+ influx at physiological extra-cellular calcium concentrations
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• double-positive thymocytes show a substantial decrease in Ca2+ flux when activated
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• there is a substantial decrease in the number of IFN-gamma producing CD4+ T cells, as well as a reduction in mean cytokine production per cell, when cultured in vitro under nave and Th1-differentiating conditions
• cytolytic CD8+ T cells also have decreased IFN-gamma production
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• there is a substantial decrease in the number of IL10-producing CD4+ T cells, as well as a reduction in mean cytokine production per cell, when cultured in vitro under Th2-differentiating conditions
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• there is a substantial decrease in the number of IL2-producing CD4+ T cells, as well as a reduction in mean cytokine production per cell, when cultured in vitro under nave and Th1-differentiating conditions
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• there is a substantial decrease in the number of IL4-producing CD4+ T cells, as well as a reduction in mean cytokine production per cell, when cultured in vitro under Th2-differentiating conditions
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• eyelid irritation is observed in all mice with variable severity
• irritation is independent of cage bedding (i.e. not related to allergies)
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• eyelid irritation is observed in all mice with variable severity
• irritation is independent of cage bedding (i.e. not related to allergies)
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• mast cells show defects in store-operated Ca2+ influx when activated by thapsigargin treatment
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• B cells show defects in store-operated Ca2+ influx when activated through their BCR, by thapsigargin treatment, or my maximally stimulating them with ionomycin
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• nave T cells show defects in store-operated Ca2+ influx only when activated in the presence of low (0.5mM) extra-cellular Ca2+
• antigen experienced T cells show defects in store-operated Ca2+ influx at physiological extra-cellular calcium concentrations
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• double-positive thymocytes show a substantial decrease in Ca2+ flux when activated
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• sporadic hair loss occurs in these mice
• mice showed a patchy pattern of hair loss, concentrated on the occipital region of the skin of the skull
• affected mouse in initially show a progressive spread of the hair-less region toward the tail, and then a partial regrowth of hair in the affected region
• with increasing age, hairless patches are replaced with sparse hair growth
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• vibrissae are shorter and thinner than controls
• vibrissae follicles are smaller and less developed
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• double-positive thymocytes show a substantial decrease in Ca2+ flux when activated
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• B cells show defects in store-operated Ca2+ influx when activated
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• CD4+ T cells show defects in store-operated Ca2+ influx when activated
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• B cells show defects in store-operated Ca2+ influx when activated
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• CD4+ T cells show defects in store-operated Ca2+ influx when activated
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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