Analysis Tools
immune system
| N |
• only mast cell (MC)-regulated exocytosis is affected while other MC effector responses remain intact, including the secretion of cytokines via constitutive exocytosis
• no significant differences in the total number, distribution or structure of MCs are observed
|
|
• plasma membrane capacitance (Cm) recordings of single peritoneal MCs after stimulation by intracellular dialysis of GTPgammaS and Ca2+ show that MCs achieve the same total increase in plasma membrane area (deltaCm) at a significantly lower rate than control MCs; however, time between cell access and beginning of the exocytic burst is normal
• ~70% of single 'steps' in the Cm curve are equal to or less than 12 fF versus ~35% in control MCs, whereas large steps are almost completely absent, indicating a shift to exocytosis of smaller-sized vesicles; residual exocytosis thus proceeds at a slower pace and is composed primarily of single-vesicle fusion events
• after stimulation with PMA/ionomycin, peritoneal MCs show altered exocytosis-related ultrastructural changes with an intermediate gain in electron lucency of their granules and a nearly complete absence of intracellular multigranular compartments, indicating that MCs are unable to engage multigranular compound exocytosis
|
|
• in a model of passive systemic anaphylaxis, mice sensitized i.p. with anti-DNP IgE and challenged i.p. with DNP-HAS exhibit histological evidence that MC degranulation in connective tissues is significantly lower than in control MCs at 90 min post-challenge
• however, the MC-dependent hypothermic response is normal at 50 min post-challenge
|
hematopoietic system
|
• plasma membrane capacitance (Cm) recordings of single peritoneal MCs after stimulation by intracellular dialysis of GTPgammaS and Ca2+ show that MCs achieve the same total increase in plasma membrane area (deltaCm) at a significantly lower rate than control MCs; however, time between cell access and beginning of the exocytic burst is normal
• ~70% of single 'steps' in the Cm curve are equal to or less than 12 fF versus ~35% in control MCs, whereas large steps are almost completely absent, indicating a shift to exocytosis of smaller-sized vesicles; residual exocytosis thus proceeds at a slower pace and is composed primarily of single-vesicle fusion events
• after stimulation with PMA/ionomycin, peritoneal MCs show altered exocytosis-related ultrastructural changes with an intermediate gain in electron lucency of their granules and a nearly complete absence of intracellular multigranular compartments, indicating that MCs are unable to engage multigranular compound exocytosis
|
|
• in a model of passive systemic anaphylaxis, mice sensitized i.p. with anti-DNP IgE and challenged i.p. with DNP-HAS exhibit histological evidence that MC degranulation in connective tissues is significantly lower than in control MCs at 90 min post-challenge
• however, the MC-dependent hypothermic response is normal at 50 min post-challenge
|
mortality/aging
| N |
• mice are viable, grossly normal, and exhibit a normal life span when raised in a specific pathogen-free facility
|
cellular
|
• plasma membrane capacitance (Cm) recordings of single peritoneal MCs after stimulation by intracellular dialysis of GTPgammaS and Ca2+ show that MCs achieve the same total increase in plasma membrane area (deltaCm) at a significantly lower rate than control MCs; however, time between cell access and beginning of the exocytic burst is normal
• ~70% of single 'steps' in the Cm curve are equal to or less than 12 fF versus ~35% in control MCs, whereas large steps are almost completely absent, indicating a shift to exocytosis of smaller-sized vesicles; residual exocytosis thus proceeds at a slower pace and is composed primarily of single-vesicle fusion events
• after stimulation with PMA/ionomycin, peritoneal MCs show altered exocytosis-related ultrastructural changes with an intermediate gain in electron lucency of their granules and a nearly complete absence of intracellular multigranular compartments, indicating that MCs are unable to engage multigranular compound exocytosis
|
|
• in a model of passive systemic anaphylaxis, mice sensitized i.p. with anti-DNP IgE and challenged i.p. with DNP-HAS exhibit histological evidence that MC degranulation in connective tissues is significantly lower than in control MCs at 90 min post-challenge
• however, the MC-dependent hypothermic response is normal at 50 min post-challenge
|
