About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(Isl1-EGFP*)1Slp
transgene insertion 1, Samuel L Pfaff
MGI:3779015
Summary 13 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
colm/colm
Tg(Isl1-EGFP*)1Slp/0
B6.Cg-colm Tg(Isl1-EGFP*)1Slp MGI:3779034
cx2
crts/crts
Tg(Isl1-EGFP*)1Slp/0
B6.Cg-crts Tg(Isl1-EGFP*)1Slp MGI:3779033
cx3
dleon/dleon
Tg(Isl1-EGFP*)1Slp/0
B6.Cg-dleon Tg(Isl1-EGFP*)1Slp MGI:3779032
cx4
Mycbp2magsp/Mycbp2magsp
Tg(Isl1-EGFP*)1Slp/0
B6.Cg-Mycbp2magsp Tg(Isl1-EGFP*)1Slp MGI:3779027
cx5
Mycbp2mgln/Mycbp2mgln
Tg(Isl1-EGFP*)1Slp/0
B6.Cg-Mycbp2mgln Tg(Isl1-EGFP*)1Slp MGI:3779030
cx6
piza/piza
Tg(Isl1-EGFP*)1Slp/0
B6.Cg-pizaa Tg(Isl1-EGFP*)1Slp MGI:3779026
cx7
Vesp/?
Tg(Isl1-EGFP*)1Slp/0
B6.Cg-Vesp Tg(Isl1-EGFP*)1Slp MGI:3779031
cx8
Kif21atm1.1Ece/Kif21a+
Map1btm1Prop/Map1b+
Tg(Isl1-EGFP*)1Slp/0
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * BALB/c * C57BL/6 MGI:6241441
cx9
Kif21atm1.1Ece/Kif21atm1.1Ece
Tg(Isl1-EGFP*)1Slp/0
involves: 129S1/Sv * 129S4/SvJae * BALB/c * C57BL/6 MGI:6241436
cx10
Chn1tm1.1Ece/Chn1tm1.1Ece
Tg(Isl1-EGFP*)1Slp/0
involves: 129S1/Sv * 129S6/SvEvTac * BALB/c * C57BL/6J MGI:6406399
cx11
Map1btm1Prop/Map1btm1Prop
Tg(Isl1-EGFP*)1Slp/0
involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6 MGI:6241439
cx12
Mafbtm1.2Good/Mafbtm1.2Good
Tg(Isl1-EGFP*)1Slp/0
involves: 129S4/SvJae * BALB/c * C57BL/6 MGI:6278261
cx13
Mafbtm1.2Good/Mafb+
Tg(Isl1-EGFP*)1Slp/0
involves: 129S4/SvJae * BALB/c * C57BL/6 MGI:6278262


Genotype
MGI:3779034
cx1
Allelic
Composition
colm/colm
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
B6.Cg-colm Tg(Isl1-EGFP*)1Slp
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
colm mutation (0 available); any colm mutation (0 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• motor neuron axons fail to form discrete ventral roots as they exit the spinal cord
• mice exhibit improper motor neuron column formation




Genotype
MGI:3779033
cx2
Allelic
Composition
crts/crts
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
B6.Cg-crts Tg(Isl1-EGFP*)1Slp
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
crts mutation (0 available); any crts mutation (0 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• mice exhibit abnormal motor neuron column formation at the lumbar level of the spinal cord




Genotype
MGI:3779032
cx3
Allelic
Composition
dleon/dleon
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
B6.Cg-dleon Tg(Isl1-EGFP*)1Slp
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
dleon mutation (0 available); any dleon mutation (0 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• mice exhibit motor neuron axons that project aberrantly shortly after exiting the lumbar spinal cord




Genotype
MGI:3779027
cx4
Allelic
Composition
Mycbp2magsp/Mycbp2magsp
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
B6.Cg-Mycbp2magsp Tg(Isl1-EGFP*)1Slp
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mycbp2magsp mutation (0 available); any Mycbp2 mutation (152 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• mice exhibit severe motor neuron axon guidance defects proximal to the spinal cord




Genotype
MGI:3779030
cx5
Allelic
Composition
Mycbp2mgln/Mycbp2mgln
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
B6.Cg-Mycbp2mgln Tg(Isl1-EGFP*)1Slp
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mycbp2mgln mutation (0 available); any Mycbp2 mutation (152 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• growth cones fail to exhibit the normal phases of outgrowth
• the intercostals motor nerves are reduced in thickness compared to in wild-type mice likely due to reduced numbers of axons present at the nerve
• all mice exhibit severe projection errors in the ventral root with highly fluorescent GFP puncta at the thoracic and lumbar levels of the spinal cord
• mice exhibit limb innervation defects
• branchial motor neuron axons misproject from the ventral root and inappropriately enter the dorsal root ganglion
• lumbar motor neuron axons wander or stall within 100 um of exiting the spinal cord
• upper thoracic motor neuron axons exhibit stalling and misprojection
• sensory neurons are thinner than in wild-type mice
• sensory neuron axons are overgrown and defasciculated resulting in thinner nerves at more distal locations compared to in wild-type mice
• the ventral ramus at the thoracic level is reduced in size while the posterior ramus is more prominent

cellular
• growth cones fail to exhibit the normal phases of outgrowth




Genotype
MGI:3779026
cx6
Allelic
Composition
piza/piza
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
B6.Cg-pizaa Tg(Isl1-EGFP*)1Slp
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
piza mutation (0 available); any piza mutation (0 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• mice exhibit motor neuron axon misprojections specifically at the thoracic level of the spinal cord




Genotype
MGI:3779031
cx7
Allelic
Composition
Vesp/?
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
B6.Cg-Vesp Tg(Isl1-EGFP*)1Slp
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Isl1-EGFP*)1Slp mutation (1 available)
Vesp mutation (0 available); any Vesp mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• mice exhibit motor neuron axon misprojections specifically at the thoracic level of the spinal cord




Genotype
MGI:6241441
cx8
Allelic
Composition
Kif21atm1.1Ece/Kif21a+
Map1btm1Prop/Map1b+
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Kif21atm1.1Ece mutation (0 available); any Kif21a mutation (57 available)
Map1btm1Prop mutation (0 available); any Map1b mutation (58 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• approximately 90% penetrance of abducens nerve hypoplasia
• oculomotor nerve superior branch axons terminate prematurely within a bulb with a penetrance of 90%




Genotype
MGI:6241436
cx9
Allelic
Composition
Kif21atm1.1Ece/Kif21atm1.1Ece
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
involves: 129S1/Sv * 129S4/SvJae * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Kif21atm1.1Ece mutation (0 available); any Kif21a mutation (57 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• extraocular muscle hypoplasia begins after P0, several days after extraocular muscle innervation is reduced, with normal position and size of the superior rectus and superior rectus muscles at E14.5

nervous system
• oculomotor nerve neurons show increased apoptosis from E12.5-E15.5
• oculomotor nerve neurons show failure of axon elongation to the orbit; this nerve growth failure precedes motor neuron apoptosis
• developing axons of the oculomotor nerves superior division stall in the proximal nerve, and the growth cones enlarge, extend excessive filopodia, and assume random trajectories while inferior division axons reach the orbit but branch ectopically
• oculomotor explant axons have normal growth but enlarged growth cones and increased filopodia
• the distal abducens nerves appear thin at E12.5 and are thinner at E15.5
• oculomotor nerve superior branch axons terminate prematurely within a bulb; the bulb contains misdirected axons with enlarged growth cones and increased number of filopodia and degenerating axons
• the developing distal oculomotor nerve superior division is hypoplastic while the inferior division develops aberrant branches
• the oculomotor nerve distal sections contain 55% fewer axons than proximal sections, and the proximal sections contain 18% fewer axons than wild-type proximal sections
• oculomotor nerve pathology does not arise from a primary defect in extraocular muscle development, axon retraction, or motor neuron cell death
• thinning of distal oculomotor nerves at E11.5
• the developing oculomotor nerves superior division is thinner than that of wild-type mice, while the oculomotor nerves inferior division appears moderately thinner, with premature fasciculation into transient aberrant branches

muscle
• extraocular muscle hypoplasia begins after P0, several days after extraocular muscle innervation is reduced, with normal position and size of the superior rectus and superior rectus muscles at E14.5

cellular
• oculomotor nerve neurons show increased apoptosis from E12.5-E15.5
• oculomotor nerve neurons show failure of axon elongation to the orbit; this nerve growth failure precedes motor neuron apoptosis
• developing axons of the oculomotor nerves superior division stall in the proximal nerve, and the growth cones enlarge, extend excessive filopodia, and assume random trajectories while inferior division axons reach the orbit but branch ectopically

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
congenital fibrosis of the extraocular muscles DOID:0080143 OMIM:PS135700
J:213171




Genotype
MGI:6406399
cx10
Allelic
Composition
Chn1tm1.1Ece/Chn1tm1.1Ece
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
involves: 129S1/Sv * 129S6/SvEvTac * BALB/c * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chn1tm1.1Ece mutation (0 available); any Chn1 mutation (38 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• abducens nerve stalls and fails to innervate the lateral rectus and is absent from the orbit at E16.5
• oculomotor nerve aberrantly innervates the lateral rectus muscle, indicating compensatory innervation
• however, oculomotor nerve has a normal trajectory to the orbit
• the number of abducens motor neurons is greatly reduced by E13.5, but is normal at E10.5, immediately prior to abducens stalling, indicating secondary motor neuron apoptosis by E13.5




Genotype
MGI:6241439
cx11
Allelic
Composition
Map1btm1Prop/Map1btm1Prop
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Map1btm1Prop mutation (0 available); any Map1b mutation (58 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• 90% of oculomotor nerves have mild proximal thickening in the absence of a bulb, distal thinning, hypoplasia of the oculomotor nerve superior division and a smaller oculomotor nerve inferior division
• about 30% of distal oculomotor nerves have aberrant, long, fasciculated branches that emerge from the oculomotor nerve inferior division exploratory region




Genotype
MGI:6278261
cx12
Allelic
Composition
Mafbtm1.2Good/Mafbtm1.2Good
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
involves: 129S4/SvJae * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mafbtm1.2Good mutation (0 available); any Mafb mutation (12 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• E11.5 embryos show severe malformations of the hindbrain, with loss of rhombomeres 5 and 6
• embryos show aberrant extraocular muscle innervation
• by E12.5, an aberrant branch of the oculomotor nerve forms and contacts the developing lateral rectus muscle along the normal abducens nerve trajectory and other aberrant branches develop and contact the retractor bulbi muscle
• at E13.5, the oculomotor nerve forms a second, more distal aberrant branch that extends toward the lateral rectus muscle in addition to the more proximal aberrant branches contacting the lateral rectus and retractor bulbi muscles
• by E16.5, the lateral rectus muscle receives innervation form the distinct proximal and distal aberrant oculomotor nerve branches
• the diameter of the distal aberrant branch of the oculomotor nerve is greater than that of the proximal aberrant branch
• abducens nerves are missing in E11.5 embryos
• E11.5 embryos show fusion of the glossopharyngeal and vagus nerves
• oculomotor nerve forms aberrant branches that contact the retractor bulbi and lateral rectus muscles at E12.5 to E16.5

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
Duane retraction syndrome DOID:12557 OMIM:126800
OMIM:604356
J:237642




Genotype
MGI:6278262
cx13
Allelic
Composition
Mafbtm1.2Good/Mafb+
Tg(Isl1-EGFP*)1Slp/0
Genetic
Background
involves: 129S4/SvJae * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mafbtm1.2Good mutation (0 available); any Mafb mutation (12 available)
Tg(Isl1-EGFP*)1Slp mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• by E12.5, aberrant branches of the oculomotor nerve begin to form in the direction of the lateral rectus muscle and retractor bulbi muscle
• at E13.5, the oculomotor nerve forms a second, more distal aberrant branch that extends toward the lateral rectus muscle in addition to the more proximal aberrant branches contacting the lateral rectus and retractor bulbi muscles
• by E16.5, the abducens nerve remains hypoplastic but provides some innervation to the lateral rectus muscle
• by E16.5, the lateral rectus muscle receives innervation form the distinct proximal and distal aberrant oculomotor nerve branches
• the diameter of the distal aberrant branch of the oculomotor nerve is greater than that of the proximal aberrant branch
• E11.5 embryos show hypoplastic abducens nerves

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
Duane retraction syndrome DOID:12557 OMIM:126800
OMIM:604356
J:237642





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
01/18/2022
MGI 6.17
The Jackson Laboratory