Phenotypes associated with this allele

• Allele Symbol: Rtl1^tm1Fuis
• Allele Name: targeted mutation 1, Fumitoshi Ishino
• Allele ID: MGI:3776873
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Rtl1^tm1Fuis/Rtl1^+	involves: 129S/SvEv * C57BL/6	MGI:3810502
ht2	Rtl1^tm1Fuis/Rtl1^+	involves: 129S/SvEv * DBA/2	MGI:3810503

Genotype
MGI:3810502

ht1

• Allelic Composition: Rtl1^tm1Fuis/Rtl1⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased survivor rate ( J:132647 )

• Background Sensitivity: when this allele is inherited maternally, mice exhibit decreasing survival rates with successive backcrossing to C57BL/6

lethality throughout fetal growth and development, incomplete penetrance ( J:132647 )

• Background Sensitivity: when this allele is inherited paternally, mice exhibit progressive fetal lethality that increases in successive generations of breeding to C57BL/6 mice

• when this allele is inherited paternally, 50% of mice die between E15.5 and E19

neonatal lethality, complete penetrance ( J:132647 )

• when this allele is inherited paternally, mice born by caesarian or vaginally die within a day

• Background Sensitivity: when this allele is inherited maternally, mice exhibit progressive neonatal lethality that increases through successive generations of breeding to C57BL/6 mice beginning at the fourth generation of breeding

• when this allele is inherited maternally, mice die within a day of birth

embryo

abnormal placenta labyrinth morphology ( J:132647 )

• when this allele is inherited paternally, at E15.5 the labyrinth exhibits spitting of the basement membrane and clogging in the fetal capillaries unlike in wild type mice

abnormal placenta size ( J:132647 )

decreased placenta weight ( J:132647 )

• when this allele is inherited paternally

enlarged placenta ( J:132647 )

• when this allele is inherited maternally, mice exhibit a 150% enlargement in the placenta and the inner spaces of the fetal capillaries are expanded compared to in wild-type mice

increased placenta weight ( J:132647 )

• when this allele is inherited maternally

abnormal trophoblast layer morphology ( J:132647 )

• when this allele is inherited paternally, the surrounding layer III trophoblast cells exhibit diffuse CD31 staining and contain abnormal lysosomes unlike in wild-type mice indicating phagocytosis reactivity towards endothelial cells of the placenta

• when this allele is inherited maternally, the surrounding layer III trophoblast cells contain a large number of vacuoles unlike in wild-type mice

abnormal placenta development ( J:132647 )

• when this allele is inherited paternally, placental development is delayed

impaired placental function ( J:132647 )

• when this allele is inherited paternally, passive permeability of insulin is decreased 48% compared to in wild-type placenta

• however, active placenta transport is normal

growth/size/body

fetal growth retardation ( J:132647 )

• when this allele is inherited paternally

cellular

abnormal basement membrane morphology ( J:132647 )

• when this allele is inherited paternally, at E15.5 the labyrinth exhibits spitting of the basement membrane

Genotype
MGI:3810503

ht2

• Allelic Composition: Rtl1^tm1Fuis/Rtl1⁺
• Genetic Background: involves: 129S/SvEv * DBA/2

mortality/aging

decreased survivor rate ( J:132647 )

• Background Sensitivity: when this allele is inherited paternally, mice that survive P1 grow normally unlike on a C57BL/6 background

lethality throughout fetal growth and development, incomplete penetrance ( J:132647 )

• when this allele is inherited paternally, some mice die in utero

neonatal lethality, incomplete penetrance ( J:132647 )

• when this allele is inherited paternally, some mice die shortly after birth