Phenotypes associated with this allele

• Allele Symbol: Tcra/Tcrd^{tm1.1(Tcra)Rsky}
• Allele Name: targeted mutation 1.1, Klaus Rajewsky
• Allele ID: MGI:3773840
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tcra^tm1Mom/Tcra/Tcrd^tm1.1(Tcra)Rsky H2^b/H2^b Tg(Tcrb)93Vbo/?	involves: 129P2/OlaHsd * C57BL/6J * DBA/2J	MGI:3807222
cx2	H2^b/H2^b Tcra/Tcrd^tm1.1(Tcra)Rsky/Tcra/Tcrd^tm1.1(Tcra)Rsky Tg(Tcrb)93Vbo/?	involves: 129P2/OlaHsd * C57BL/6J * DBA/2J	MGI:3807224
cx3	Tcra^tm1Mom/Tcra/Tcrd^tm1.1(Tcra)Rsky H2^d/H2^d Ptcra^tm1Vbo/Ptcra^tm1Vbo Tg(Tcrb)93Vbo/?	involves: 129P2/OlaHsd * C57BL/6J * DBA/2J	MGI:3807288

Genotype
MGI:3807222

cx1

• Allelic Composition: Tcra^tm1Mom/Tcra/Tcrd^{tm1.1(Tcra)Rsky}; H2^b/H2^b; Tg(Tcrb)93Vbo/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * DBA/2J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased thymocyte number ( J:131357 )

abnormal T cell differentiation ( J:81564 )

• 18% of lymph node T cells from female mice bearing H2-D^b express the HY-alpha t cell receptor chain

• the majority of T cells in male mice are HYalpha⁺ CD4^-CD8^lo or HYalpha⁺ CD4^-CD8^-

• 5 of 29 HY^- thymocytes from H2-D^b background mice have some receptor editing though the small number of DP and SP thymocytes present suggests this process is inefficient

abnormal negative T cell selection ( J:81564 )

• thymocytes from male mice on a H2-D^b background that express the HY receptor are CD69⁺ and undergoing apoptosis due to negative selection

abnormal double-negative T cell morphology ( J:131357 )

• DN thymocytes of both sexes have premature expression of a T cell receptor (TCR)

• this TCR is specific for the male HY antigen and in many cases there is co-expression of CD25 and CD44

• the percentage of DN cells in the thymus is increased

abnormal double-positive T cell morphology ( J:81564 )

• female mice on a H2-D^b background have almost double the number of DP thymocytes compared to female mice carrying just the Tcrbeta transgene

• the majority of these DP thymocytes do not carry the HY TCR

• male mice on a H2-D^b background have 4% the number of double positive thymocytes

decreased double-positive T cell number ( J:131357 )

immune system

decreased thymocyte number ( J:131357 )

abnormal T cell differentiation ( J:81564 )

• 18% of lymph node T cells from female mice bearing H2-D^b express the HY-alpha t cell receptor chain

• the majority of T cells in male mice are HYalpha⁺ CD4^-CD8^lo or HYalpha⁺ CD4^-CD8^-

• 5 of 29 HY^- thymocytes from H2-D^b background mice have some receptor editing though the small number of DP and SP thymocytes present suggests this process is inefficient

abnormal negative T cell selection ( J:81564 )

• thymocytes from male mice on a H2-D^b background that express the HY receptor are CD69⁺ and undergoing apoptosis due to negative selection

abnormal double-negative T cell morphology ( J:131357 )

• DN thymocytes of both sexes have premature expression of a T cell receptor (TCR)

• this TCR is specific for the male HY antigen and in many cases there is co-expression of CD25 and CD44

• the percentage of DN cells in the thymus is increased

abnormal double-positive T cell morphology ( J:81564 )

• female mice on a H2-D^b background have almost double the number of DP thymocytes compared to female mice carrying just the Tcrbeta transgene

• the majority of these DP thymocytes do not carry the HY TCR

• male mice on a H2-D^b background have 4% the number of double positive thymocytes

decreased double-positive T cell number ( J:131357 )

endocrine/exocrine glands

decreased thymocyte number ( J:131357 )

Genotype
MGI:3807224

cx2

• Allelic Composition: H2^b/H2^b; Tcra/Tcrd^{tm1.1(Tcra)Rsky}/Tcra/Tcrd^{tm1.1(Tcra)Rsky}; Tg(Tcrb)93Vbo/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * DBA/2J

hematopoietic system

abnormal T cell differentiation ( J:81564 )

• male mice on a H2-D^b background have no distinct double positive or double negative populations in their thymus

abnormal negative T cell selection ( J:81564 )

• almost all thymocytes in male mice on a H2-D^b background undergo negative selection

• receptor editing is unable to rescue these thymocytes

immune system

abnormal T cell differentiation ( J:81564 )

• male mice on a H2-D^b background have no distinct double positive or double negative populations in their thymus

abnormal negative T cell selection ( J:81564 )

• almost all thymocytes in male mice on a H2-D^b background undergo negative selection

• receptor editing is unable to rescue these thymocytes

Genotype
MGI:3807288

cx3

• Allelic Composition: Tcra^tm1Mom/Tcra/Tcrd^{tm1.1(Tcra)Rsky}; H2^d/H2^d; Ptcra^tm1Vbo/Ptcra^tm1Vbo; Tg(Tcrb)93Vbo/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * DBA/2J

hematopoietic system

decreased thymocyte number ( J:131357 )

• thymocyte numbers are reduced by about 3-fold

abnormal T cell differentiation ( J:131357 )

• the presence of HY TCR alleviates the beta-selection block in Ptcra^tm1Vbo homozygotes with higher T cell numbers being found in the thymus and the spleen

• despite the increased numbers of thymocytes, the CD25⁺ thymocyte sub-population still remains very low

immune system

decreased thymocyte number ( J:131357 )

• thymocyte numbers are reduced by about 3-fold

abnormal T cell differentiation ( J:131357 )

• the presence of HY TCR alleviates the beta-selection block in Ptcra^tm1Vbo homozygotes with higher T cell numbers being found in the thymus and the spleen

• despite the increased numbers of thymocytes, the CD25⁺ thymocyte sub-population still remains very low

endocrine/exocrine glands

decreased thymocyte number ( J:131357 )

• thymocyte numbers are reduced by about 3-fold