Phenotypes associated with this allele

• Allele Symbol: Tg(Lck-Jak3)2Ljb
• Allele Name: transgene insertion 2, Leslie J Berg
• Allele ID: MGI:3769343
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Jak3^tm1Ljb/Jak3^tm1Ljb Tg(Lck-Jak3)2Ljb/0	involves: 129S4/SvJae * C3H * C57BL/6	MGI:3769347
cx2	Jak3^tm1Ljb/Jak3^tm1Ljb Tg(Lck-Jak3)2Ljb/0	involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10	MGI:3769356

Genotype
MGI:3769347

cx1

• Allelic Composition: Jak3^tm1Ljb/Jak3^tm1Ljb; Tg(Lck-Jak3)2Ljb/0
• Genetic Background: involves: 129S4/SvJae * C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:56629 )

N • unlike in homozygous null mice that do not express the transgene, the numbers of CD4 and CD8 cells in the spleen and thymus are normal

enlarged spleen ( J:56629 )

• splenomegaly apparent after 12 weeks of age

• spleen size is 2 to 3 times that of age-matched homozygous null mice that do not express the transgene

increased leukocyte cell number ( J:56629 )

• an expansion of large granular cells is seen, similar to homozygous null mice that do not express the transgene

abnormal T cell activation ( J:56629 )

• CD4+ cells show an activated phenotype

hematopoietic system

enlarged spleen ( J:56629 )

• splenomegaly apparent after 12 weeks of age

• spleen size is 2 to 3 times that of age-matched homozygous null mice that do not express the transgene

increased leukocyte cell number ( J:56629 )

• an expansion of large granular cells is seen, similar to homozygous null mice that do not express the transgene

abnormal T cell activation ( J:56629 )

• CD4+ cells show an activated phenotype

growth/size/body

enlarged spleen ( J:56629 )

• splenomegaly apparent after 12 weeks of age

• spleen size is 2 to 3 times that of age-matched homozygous null mice that do not express the transgene

Genotype
MGI:3769356

cx2

• Allelic Composition: Jak3^tm1Ljb/Jak3^tm1Ljb; Tg(Lck-Jak3)2Ljb/0
• Genetic Background: involves: 129S4/SvJae * C3H * C57BL/6 * C57BL/10

immune system

immune system phenotype ( J:128694 )

N • unlike null mice without the transgene, thymic cellularity, the ratio of CD4+ to CD8+ cells in the thymus, T cell maturation in the spleen, and the numbers of gamma delta T and NK cells are normal

absent B cells ( J:128694 )

• absence of CD45R+ IgM+ cells in the bone marrow

abnormal T cell physiology ( J:128694 )

• the ability of splenic T cells to secrete IL12 in response to T cell receptor plus CD28 stimulation decreases with age (from 25 to 42 days of age)

abnormal T cell activation ( J:128694 )

• as mice age the proportion of CD4+ T cells that resemble activated or memory T cells increases

• this correlates with the decrease in transgene expression in the periphery as mice age

decreased T cell proliferation ( J:128694 )

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced

absent lymph nodes ( J:128694 )

hematopoietic system

absent B cells ( J:128694 )

• absence of CD45R+ IgM+ cells in the bone marrow

abnormal T cell physiology ( J:128694 )

• the ability of splenic T cells to secrete IL12 in response to T cell receptor plus CD28 stimulation decreases with age (from 25 to 42 days of age)

abnormal T cell activation ( J:128694 )

• as mice age the proportion of CD4+ T cells that resemble activated or memory T cells increases

• this correlates with the decrease in transgene expression in the periphery as mice age

decreased T cell proliferation ( J:128694 )

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced

cellular

decreased T cell proliferation ( J:128694 )

• the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is reduced