Analysis Tools
mortality/aging
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• 22 of 24 mice die after 20 hours of E. coli infection compared to only 7 of 24 wild-type mice
• however, the remaining 2 mice recover completely while 9 of 24 wild-type mice recover
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immune system
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• mice are neutropenic but improve with age
• at 3 weeks, mice have 36% of the neutrophils found in wild-type mice
• at 7 weeks, mice have 67% of the neutrophils found in wild-type mice
• in thiocollate-injected mice, neutrophil accumulation is only 52% of that in wild-type mice
• however, bone marrow hematopoiesis is normal
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• 1.6-, 1.6-, and 1.7-fold increase in granulocyte, granulocyte-macrophage, and macrophage colony forming units, respectively, following stimulation with G-CSF, GM-CSF, or M-CSF
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• thiocollate-treated mice have reduced PMA-stimulated superoxide production (indicative of decreased respiratory burst activity)
and less dose-dependent chemotratic response to fMLP and the chemokines cytokine-induced neutrophil chemoattractant (KC)
• calcium mobilization is impaired
• neutrophils undergo increased apoptosis (8-12% of peritoneal neutrophils bind annexin V, while few, if any, wild-type cells do, and caspase-3 activation is increased)
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• increase in bacterial count 5 hours after E. coli infection in mice that die early compared to those that die late
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• 22 of 24 mice die after 20 hours of E. coli infection compared to only 7 of 24 wild-type mice
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hematopoietic system
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• mice are neutropenic but improve with age
• at 3 weeks, mice have 36% of the neutrophils found in wild-type mice
• at 7 weeks, mice have 67% of the neutrophils found in wild-type mice
• in thiocollate-injected mice, neutrophil accumulation is only 52% of that in wild-type mice
• however, bone marrow hematopoiesis is normal
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• 1.6-, 1.6-, and 1.7-fold increase in granulocyte, granulocyte-macrophage, and macrophage colony forming units, respectively, following stimulation with G-CSF, GM-CSF, or M-CSF
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• thiocollate-treated mice have reduced PMA-stimulated superoxide production (indicative of decreased respiratory burst activity)
and less dose-dependent chemotratic response to fMLP and the chemokines cytokine-induced neutrophil chemoattractant (KC)
• calcium mobilization is impaired
• neutrophils undergo increased apoptosis (8-12% of peritoneal neutrophils bind annexin V, while few, if any, wild-type cells do, and caspase-3 activation is increased)
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• increase in bacterial count 5 hours after E. coli infection in mice that die early compared to those that die late
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