Analysis Tools
mortality/aging
 |
• no Dlg3tm1Grnt/y, Dlg4-null male mice mice are produced by crosses of heterozygous Dlg3 and Dlg4 mice; double mutants die perinatally
|
|
Allele Symbol Allele Name Allele ID |
Dlg3tm1Grnt targeted mutation 1, Seth G N Grant MGI:3706312 |
||||||||||||||||||||||||
| Summary |
5 genotypes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
• no Dlg3tm1Grnt/y, Dlg4-null male mice mice are produced by crosses of heterozygous Dlg3 and Dlg4 mice; double mutants die perinatally
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 |
• crosses of heterozygous Dlg3 and Dlg4 mice produce a very low proportion of Dlg3tm1Grnt/+, Dlg4-null female mice
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
• crosses of heterozygous Dlg3 and Dlg4 mice produce no double homozygotes; double mutants die perinatally with none surviving past day 3 postnatal
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
• brain mass is reduced
|
|
|
• total neocortical area is reduced
|
|
|
• reduction in the total numbers of thalamocortical axons innervating the somatosensory cortex
• however, segregation of barrels is unaffected
|
|
|
• area of posteromedial barrel subfield is reduced
• combined area of all thalamocortical axon patches in the barrel cortex is reduced indicating a reduction in thalamocortical innervation of barrel cortex
|
|
|
• P8-10 mice show a reduction in connectivity of individual thalamocortical axons onto L4 neurons despite normal NMDA receptor function after the critical period
• reduction in neurofilament medium polypeptide-positive axons in barrel cortex in P6-7 mice
|
|
|
• decrease in the number of thalamocortical axons
|
|
|
• thalamocortical synapses transiently show altered NMDA receptor function
• however, after the critical period thalamocortical synapses function normally
|
|
|
• reduction in peak excitatory postsynaptic current (EPSC) amplitude
• however, no differences are seen in minimal stimulation EPSC amplitude
|
|
|
• NMDA excitatory postsynaptic currents (EPSCs) show faster decay kinetics during the critical period for thalamocortical plasticity
• treatment with ifenprodil, a GluN2B-specific antagonist, has no effect on NMDA EPSCs
|
Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| non-syndromic X-linked intellectual disability | DOID:0050776 |
OMIM:300716 OMIM:PS309530 |
J:238576 | |
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
|
• in Morris water maze test, with a visible, elevated platform, mutants' speed in reaching platform improves on day 2 and 3 of training whereas wild-type mice do not improve
• in hidden platform paradigm, wild-type mice show improvement in performance over 5 days of training, while mutants do not
• in a reversal platform protocol, mutants show a gradual improvement after 6 days, while wild-type learn the new platform position in the first block of training; retention of information on platform position is retained for 2 weeks, but by 8 weeks after training, mutants' performance is back to chance levels
• in visible version of task, mutants use the approaching strategy, while wild-type use a less efficient self-orienting strategy; in the hidden (ie. hippocampus-dependent) version, mutants use a less efficient circling strategy, whereas wild-type use the approaching strategy, and with training switch to the direct finding strategy
|
|
|
• in assessment of motor abilities, mutants show an slightly altered locomotor phenotype measured by some differences in the rotarod, open field and elevated plus maze tests
|
|
|
• induction of LTP with conventional high-frequency protocol is similar in wild-type and mutant hippocampal slices; long-low-frequency train of synaptic stimulation produces a 2-fold increase in synaptic strength in mutant slices compared to only modest LTP in wild-type slices
• pairing of single pulses of presynaptic fiber stimulation with single postsynaptic action potentials induces robust LTP in mutant cells but does not in wild-type cells
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 04/16/2024 MGI 6.23 |
|
|
|
||