All Phenotypes Rb1cc1<tm1.2Guan> MGI Mouse

lethality throughout fetal growth and development, complete penetrance ( J:114498 )

• embryonic lethality between E13.5 and E16.5, with 25% dead at E14.5 and 60% at E15.5; no live embryos are recovered after E15.5

abnormal heart morphology ( J:114498 )

• exhibit an approximate 33% reduction in mean cell size in the heart

• exhibit significantly increased apoptosis in the heart at E14.5 but not at E12.5

abnormal myocardium layer morphology ( J:114498 )

• sparsely cellular myocardium

abnormal myocardial trabeculae morphology ( J:114498 )

• the thin myocardial wall is composed of wisps of thin trabecular myocardium

abnormal trabecula carnea morphology ( J:114498 )

• heart ventricular wall has lost the normal trabecular and external compact myocytes

abnormal myocardium compact layer morphology ( J:114498 )

• in the most severely affected mutants, the myocardium is composed almost entirely of the trabecular layer with no appreciable compact layer beneath the epicardium

thin myocardium ( J:114498 )

abnormal heart ventricle morphology ( J:114498 )

dilated heart left ventricle ( J:114498 )

thin ventricular wall ( J:114498 )

• wall is thinner and contains fewer myocytes

liver hemorrhage ( J:114498 )

• exhibit multifocal variable sized hemorrhages in between the hepatic cords

congestive heart failure ( J:114498 )

liver hemorrhage ( J:114498 )

• exhibit multifocal variable sized hemorrhages in between the hepatic cords

increased hepatocyte apoptosis ( J:114498 )

• exhibit significantly increased apoptosis in the liver at E14.5 and E15.5, predominantly in hepatocytes

abnormal liver morphology ( J:114498 )

• majority of E14.5 and E15.5 embryos exhibit liver lesions characterized by loosely arranged hepatocytes mixed with numerous red blood cells

• exhibit an approximate 25% reduction in mean cell size in the liver

abnormal hepatocyte morphology ( J:114498 )

• many hepatocyes have condensed nuclei (karyopyknosis), fragmented nuclei (karyorrhexis) or lost their nuclei (karyolysis)

decreased hepatocyte number ( J:114498 )

• liver shows loss of hepatocytes

dissociated hepatocytes ( J:114498 )

• exhibit liver lesions characterized by hepatocytes that are separated from each other

liver degeneration ( J:114498 )

edema ( J:114498 )

• generalized edema

decreased cell mass ( J:114498 )

• MEFs exhibit decreased cell size compared to wild-type

increased fibroblast apoptosis ( J:114498 )

• although MEFs under normal culture conditions do not show increased apoptosis, they do show increased susceptibility to TNFalpha-induced apoptosis