Phenotypes associated with this allele

• Allele Symbol: Tg(Tcra2B4)1Mmd
• Allele Name: transgene insertion 1, Mark M Davis
• Allele ID: MGI:3688270
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Itk^tm1Ljb/Itk^tm1Ljb Tg(Tcra2B4)1Mmd/? Tg(Tcrb2B4)1Mmd/?	involves: C3H/HeJ * C57BL/6 * C57BL/10	MGI:4354594
cx2	Tg(H2KmPCC)2939Stoe/0 Tg(Tcra2B4)1Mmd/0 Tg(Tcrb2B4)1Mmd/0	involves: C3H/HeJ * C57BL/6 * SJL	MGI:3688409
cx3	Dock2^tm1Tsas/Dock2^tm1Tsas Tg(Tcra2B4)1Mmd/? Tg(Tcrb2B4)1Mmd/?	involves: C57BL/6	MGI:3690240
tg4	Tg(Tcra2B4)1Mmd/?	involves: C3H/HeJ * C57BL/6	MGI:3835053

Genotype
MGI:4354594

cx1

• Allelic Composition: Itk^tm1Ljb/Itk^tm1Ljb; Tg(Tcra2B4)1Mmd/?; Tg(Tcrb2B4)1Mmd/?
• Genetic Background: involves: C3H/HeJ * C57BL/6 * C57BL/10

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

Genotype
MGI:3688409

cx2

• Allelic Composition: Tg(H2KmPCC)2939Stoe/0; Tg(Tcra2B4)1Mmd/0; Tg(Tcrb2B4)1Mmd/0
• Genetic Background: involves: C3H/HeJ * C57BL/6 * SJL

immune system

decreased T cell proliferation ( J:50143 )

• mice have no in vitro response to cytochrome C while spleen cells from Tcra-knockin, transgenic Tcrb-expressing mice exhibit a vigorous proliferation response

decreased thymocyte number ( J:50143 )

• number of thymocytes (2-4 x 10⁶) in tolerant mice is reduced 30 to 40-fold compared with normal TCR transgenic mice (Tg(H2KmPCC)2939Stoe-negative, TcraTcrb-positive mice; 70-90 x 10⁶ cells)

abnormal T cell differentiation ( J:50143 )

• number of thymocytes is drastically reduced and few thymocytes undergo natural development using the endogenous Tcra/transgenic Tcrb chain compared to Tcra-knockin, transgenic Tcrb-expressing mice

hematopoietic system

decreased T cell proliferation ( J:50143 )

• mice have no in vitro response to cytochrome C while spleen cells from Tcra-knockin, transgenic Tcrb-expressing mice exhibit a vigorous proliferation response

decreased thymocyte number ( J:50143 )

• number of thymocytes (2-4 x 10⁶) in tolerant mice is reduced 30 to 40-fold compared with normal TCR transgenic mice (Tg(H2KmPCC)2939Stoe-negative, TcraTcrb-positive mice; 70-90 x 10⁶ cells)

abnormal T cell differentiation ( J:50143 )

• number of thymocytes is drastically reduced and few thymocytes undergo natural development using the endogenous Tcra/transgenic Tcrb chain compared to Tcra-knockin, transgenic Tcrb-expressing mice

endocrine/exocrine glands

decreased thymocyte number ( J:50143 )

• number of thymocytes (2-4 x 10⁶) in tolerant mice is reduced 30 to 40-fold compared with normal TCR transgenic mice (Tg(H2KmPCC)2939Stoe-negative, TcraTcrb-positive mice; 70-90 x 10⁶ cells)

cellular

decreased T cell proliferation ( J:50143 )

• mice have no in vitro response to cytochrome C while spleen cells from Tcra-knockin, transgenic Tcrb-expressing mice exhibit a vigorous proliferation response

Genotype
MGI:3690240

cx3

• Allelic Composition: Dock2^tm1Tsas/Dock2^tm1Tsas; Tg(Tcra2B4)1Mmd/?; Tg(Tcrb2B4)1Mmd/?
• Genetic Background: involves: C57BL/6

immune system

decreased double-positive T cell number ( J:84581 )

• numbers reduced to about 3% of what is seen in controls

• more susceptible to apoptosis

abnormal CD4-positive, alpha beta T cell morphology ( J:84581 )

• reduced in number and ratio in relation to CD8+ T cells is also reduced

abnormal T cell physiology ( J:84581 )

• proliferative responses are reduced

hematopoietic system

decreased double-positive T cell number ( J:84581 )

• numbers reduced to about 3% of what is seen in controls

• more susceptible to apoptosis

abnormal CD4-positive, alpha beta T cell morphology ( J:84581 )

• reduced in number and ratio in relation to CD8+ T cells is also reduced

abnormal T cell physiology ( J:84581 )

• proliferative responses are reduced

Genotype
MGI:3835053

tg4

• Allelic Composition: Tg(Tcra2B4)1Mmd/?
• Genetic Background: involves: C3H/HeJ * C57BL/6

immune system

abnormal thymus cell ratio ( J:92992 )

• the ratio of double negative to double-positive thymocytes is significantly higher than in controls

decreased thymocyte number ( J:92992 )

• thymus cellularity is reduced by about 4 fold compared to controls

increased double-negative T cell number ( J:92992 )

• an increase in double-negative thymocytes is noted in these mice

decreased double-positive T cell number ( J:92992 )

• a decrease in double-negative thymocytes is noted in these mice

decreased CD4-positive, alpha-beta T cell number ( J:92992 )

• the absolute number of single-positive CD4 thymocytes is decreased

neoplasm

increased lymphoma incidence ( J:92992 )

• 19.4% of mice develop lymphoma by 6 months of age

hematopoietic system

abnormal thymus cell ratio ( J:92992 )

• the ratio of double negative to double-positive thymocytes is significantly higher than in controls

decreased thymocyte number ( J:92992 )

• thymus cellularity is reduced by about 4 fold compared to controls

increased double-negative T cell number ( J:92992 )

• an increase in double-negative thymocytes is noted in these mice

decreased double-positive T cell number ( J:92992 )

• a decrease in double-negative thymocytes is noted in these mice

decreased CD4-positive, alpha-beta T cell number ( J:92992 )

• the absolute number of single-positive CD4 thymocytes is decreased

endocrine/exocrine glands

abnormal thymus cell ratio ( J:92992 )

• the ratio of double negative to double-positive thymocytes is significantly higher than in controls

decreased thymocyte number ( J:92992 )

• thymus cellularity is reduced by about 4 fold compared to controls