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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(tetO-CDK5R1/GFP)337Lht
transgene insertion 337, Li-Huei Tsai
MGI:3653679
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
Bace1tm1Pcw/Bace1+
Tg(Camk2a-tTA)1Mmay/0
Tg(tetO-CDK5R1/GFP)337Lht/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J MGI:5634918
cx2
Tg(Camk2a-tTA)1Mmay/0
Tg(tetO-CDK5R1/GFP)337Lht/0
involves: C57BL/6J MGI:3653709
tg3
Tg(tetO-CDK5R1/GFP)337Lht/0 C57BL/6J-Tg(tetO-CDK5R1/GFP)337Lht MGI:3653715


Genotype
MGI:5634918
cx1
Allelic
Composition
Bace1tm1Pcw/Bace1+
Tg(Camk2a-tTA)1Mmay/0
Tg(tetO-CDK5R1/GFP)337Lht/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bace1tm1Pcw mutation (1 available); any Bace1 mutation (16 available)
Tg(Camk2a-tTA)1Mmay mutation (8 available)
Tg(tetO-CDK5R1/GFP)337Lht mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
N
• mice with transgene expression induced by removal of doxycycline from the food 4-6 weeks postnatal exhibit decreased amyloid beta in the hippocampus
• mice exhibit normal synaptic plasticity and long term potentiation (LTP) is fully restored following a 6-week induction
• mice exhibit normal density of synaptophysin puncta in the stratum radiatum of the hippocampus, indicating restoration of synapse density, following a 6-week induction
• brain weight is reduced in 6-week induced mice
• reduction in the number of NeuN-positive cells in hippocampal area CA1 in 6-week induced mice
• reactive astrogliosis is seen in the cortex and hippocampus in 6-week induced mice

behavior/neurological
N
• 6-week induced mice show improved associate learning in contextual fear conditioning and are indistinguishable from controls




Genotype
MGI:3653709
cx2
Allelic
Composition
Tg(Camk2a-tTA)1Mmay/0
Tg(tetO-CDK5R1/GFP)337Lht/0
Genetic
Background
involves: C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Camk2a-tTA)1Mmay mutation (8 available)
Tg(tetO-CDK5R1/GFP)337Lht mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• transgenic mice with transgene expression induced by removal of doxycycline from their food 4-6 weeks postnatal exhibit a slightly decreased body weight compared to control littermates

nervous system
• mice with transgene expression induced by removal of doxycycline from the food 4-6 weeks postnatal exhibit amyloid beta deposits in the hippocampus
• transgenic mice with induction of expression of the transgene for different time periods display progressive decrease in brain weight; with induction for 5 weeks there is a 15-20% decrease in brain weight and with more than 12 weeks of induction, the weight decrease is 30% (J:104240)
• in transgenic mice induced for 8 and 12 weeks, there is an increase in aggregations of tau protein (insoluble tau) with an increased amount of phosphorylated tau present; at 15 weeks, there is less soluble tau than in wild-type forebrains and similar results are found at 27 weeks of induction
• brains of transgenic mice induced for 12 weeks show a significant decrease in thickness and neuronal density in the hippocampus compared to controls
• 50% reduction in the number of NeuN-positive cells in hippocampal area CA1 in 6-week induced mice
• brains of transgenic mice induced for 12 weeks show a significant decrease in thickness and neuronal density in the cerebral cortex compared to controls; mice induced for 8 or 12 weeks show a 25 or 40% decrease in cortical neuronal density, respectively, whereas wild-type or non-induced transgenic mice had the same neuronal density
• induced transgenic mice at 12 weeks postnatal display significant forebrain atrophy with decrease in forebrain mass
• neurofibrillary tangle-like pathology is exhibited in brains of transgenic mice induced for 27 weeks; there are numerous intraneuronal and flame-shaped neurons positive for neurofibrillary tangle-specific proteins in the cortex and hippocampus of transgenic mice but not wild-type
• other methosds also identify neurofibrillary tangle-like structures in the cerebral cortex, hippocampus and entorhinal cortex of transgenic mice
• brains of transgenic mice induced for 8 or 12 weeks do not express markers for late stage tangles similar to what is observed in wild-type
• reactive astrogliosis is increased throughout the cortex and hippocampus of mutants evident by an increase in radial and stellate-shaped astrocytes
• reactive astrogliosis is seen in the cortex and hippocampus in 6-week induced mice
• mice exhibit reduced density of synaptophysin puncta in the stratum radiatum of the hippocampus, indicating reduced synapse density following a 6-week induction
• mice exhibit impaired synaptic plasticity following a 6-week induction
• mice exhibit diminished CA1 long term potentiation (LTP) following a 6-week induction

behavior/neurological
• associative learning in contextual fear conditioning is impaired in 6-week induced mice, with mice spending less time freezing after training than wild-type mice

homeostasis/metabolism
• mice with transgene expression induced by removal of doxycycline from the food 4-6 weeks postnatal exhibit amyloid beta deposits in the hippocampus

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
Alzheimer's disease DOID:10652 J:177847




Genotype
MGI:3653715
tg3
Allelic
Composition
Tg(tetO-CDK5R1/GFP)337Lht/0
Genetic
Background
C57BL/6J-Tg(tetO-CDK5R1/GFP)337Lht
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(tetO-CDK5R1/GFP)337Lht mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• hemizygous mice are viable, fertile, show normal size and display no behavioral abnormalities; homozygosity may be lethal





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory