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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Rb1tm1Fad
targeted mutation 1, Frederick A Dick
MGI:3624796
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Rb1tm1Fad/Rb1tm1Fad involves: 129 * C57BL/6 MGI:3640141
cx2
Rb1tm1Fad/Rb1tm1Fad
Tg(MMTVTGFB1)46Hlm/?
involves: 129 * C57BL/6 * DBA/2 MGI:4354285
cx3
Rb1tm1Fad/Rb1tm1Fad
Tg(KRT14-HPV16E7)2304Plam/0
involves: 129 * C57BL/6 * FVB MGI:3607130
cx4
Rb1tm1Fad/Rb1+
Tg(KRT14-HPV16E7)2304Plam/0
involves: 129 * C57BL/6 * FVB MGI:3607131


Genotype
MGI:3640141
hm1
Allelic
Composition
Rb1tm1Fad/Rb1tm1Fad
Genetic
Background
involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rb1tm1Fad mutation (1 available); any Rb1 mutation (84 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• increase in the occurrence of chromosomes with fusions in the centromere region
• increase in MEFs with greater than 4N DNA content
• an increase in MEFs in G2/M phase and decrease of cells in G0/G1; however G1 is not shortened, transit through S and G2 is similar to wild-type, and the spindle check point appears normal
• the number of lagging chromosomes seen during anaphase is increased and metaphase spreads contain more chromosomes with fusions in the centromere region

endocrine/exocrine glands
• some nursing mothers are unable to eject adequate supplies of milk
• most pups die by two days of age due to insufficient nutrition
• mammary glands have large, dilated ducts containing milk consisted with a defect in milk ejection
• mice have similar lobuloalveolar formation and milk composition as controls
• female mice have a significantly elevated frequency of hyperplastic ducts about 5-fold greater than controls
• branching frequency and overall ductal morphogenesis appeared normal
• mammary glands have hyperplastic growth throughout development
• hyperplasia was characterized by increased luminal epithelial cell layers, as well as invagination of the epithelium into the lumen of the duct

homeostasis/metabolism
• embryonic fibroblasts fail to decrease proliferation in response to TGF-beta1

integument
• some nursing mothers are unable to eject adequate supplies of milk
• most pups die by two days of age due to insufficient nutrition
• mammary glands have large, dilated ducts containing milk consisted with a defect in milk ejection
• mice have similar lobuloalveolar formation and milk composition as controls
• female mice have a significantly elevated frequency of hyperplastic ducts about 5-fold greater than controls
• branching frequency and overall ductal morphogenesis appeared normal
• mammary glands have hyperplastic growth throughout development
• hyperplasia was characterized by increased luminal epithelial cell layers, as well as invagination of the epithelium into the lumen of the duct

behavior/neurological
• some nursing mothers are unable to eject adequate supplies of milk
• most pups die by two days of age due to insufficient nutrition
• mammary glands have large, dilated ducts containing milk consisted with a defect in milk ejection
• mice have similar lobuloalveolar formation and milk composition as controls




Genotype
MGI:4354285
cx2
Allelic
Composition
Rb1tm1Fad/Rb1tm1Fad
Tg(MMTVTGFB1)46Hlm/?
Genetic
Background
involves: 129 * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rb1tm1Fad mutation (1 available); any Rb1 mutation (84 available)
Tg(MMTVTGFB1)46Hlm mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• mammary glands have hyperplastic growth throughout development
• hyperplasia was characterized by increased luminal epithelial cell layers, as well as invagination of the epithelium into the lumen of the duct
• this genotype demonstrates that mammary hyperplasia associated with Rb1tm1Fad homozygotes still occurs in the presence of high levels of TGF-beta1

integument
• mammary glands have hyperplastic growth throughout development
• hyperplasia was characterized by increased luminal epithelial cell layers, as well as invagination of the epithelium into the lumen of the duct
• this genotype demonstrates that mammary hyperplasia associated with Rb1tm1Fad homozygotes still occurs in the presence of high levels of TGF-beta1




Genotype
MGI:3607130
cx3
Allelic
Composition
Rb1tm1Fad/Rb1tm1Fad
Tg(KRT14-HPV16E7)2304Plam/0
Genetic
Background
involves: 129 * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rb1tm1Fad mutation (1 available); any Rb1 mutation (84 available)
Tg(KRT14-HPV16E7)2304Plam mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
N
• after exposure to 5 Gy of ionizing radiation skin cells display normal cell cycle arrest unlike in mice hemizygous for Tg(KRT14-HPV16E7)2304Plam alone

integument
• thickness of the spinous suprabasal cell layer is increased 30-50%; however, the thickness of the keratin 10- and filaggrin-positive layers is normal
• mice have normal gross skin morphology, normal growth and no signs of epidermal hyperplasia unlike in mice hemizygous for Tg(KRT14-HPV16E7)2304Plam alone




Genotype
MGI:3607131
cx4
Allelic
Composition
Rb1tm1Fad/Rb1+
Tg(KRT14-HPV16E7)2304Plam/0
Genetic
Background
involves: 129 * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rb1tm1Fad mutation (1 available); any Rb1 mutation (84 available)
Tg(KRT14-HPV16E7)2304Plam mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
N
• after exposure to 5 Gy of ionizing radiation skin cells display normal cell cycle arrest unlike in mice hemizygous for Tg(KRT14-HPV16E7)2304Plam alone

integument
• thickness of the spinous suprabasal cell layer is increased 30-50%; however, the thickness of the keratin 10- and filaggrin-positive layers is normal
• mice have normal gross skin morphology, normal growth and no signs of epidermal hyperplasia unlike in mice hemizygous for Tg(KRT14-HPV16E7)2304Plam alone





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last database update
06/28/2022
MGI 6.20
The Jackson Laboratory