Phenotypes associated with this allele

• Allele Symbol: Trh^tm1Mmt
• Allele Name: targeted mutation 1, Makoto M Taketo
• Allele ID: MGI:3620126
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trh^tm1Mmt/Trh^tm1Mmt	involves: C57BL/6 * CBA	MGI:3623225
hm2	Trh^tm1Mmt/Trh^tm1Mmt	involves: C57BL/6 * CBA * ICR	MGI:3623227

Genotype
MGI:3623225

hm1

• Allelic Composition: Trh^tm1Mmt/Trh^tm1Mmt
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal pituitary gland development ( J:59141 )

• in 8 week old mutants, hypoplasia is observed in thrytrophs but not in other pituitary hormone-producing cells

homeostasis/metabolism

decreased circulating thyroid-stimulating hormone level ( J:59141 )

• there is significantly decreased levels of TSH in the pituitary of homozygous mice

decreased circulating thyroxine level ( J:59141 )

• in mutants by postnatal day 10, the level of thyroid hormone increases only by 3-fold from level at birth compared to a 6-fold increase in wild-type animals; levels at 21 days and 8 weeks are 28.3% and 50.3% of wild-type levels, respectively

nervous system

abnormal pituitary gland development ( J:59141 )

• in 8 week old mutants, hypoplasia is observed in thrytrophs but not in other pituitary hormone-producing cells

Genotype
MGI:3623227

hm2

• Allelic Composition: Trh^tm1Mmt/Trh^tm1Mmt
• Genetic Background: involves: C57BL/6 * CBA * ICR

growth/size/body

postnatal growth retardation ( J:43657 )

• at 4 weeks, mice are slightly growth retarded (14.5g) compared to wild-type (20.8g); by 8 weeks of age, there is no significant difference

homeostasis/metabolism

decreased insulin secretion ( J:43657 )

• in response to 10 and 20 mM glucose, mutant mice show much lower insulin secretion than wild-type mice

hyperglycemia ( J:43657 )

• fasting blood glucose levels are higher in mutants

decreased circulating insulin level ( J:43657 )

• levels are comparatively low with respect to the elevated glucose levels

increased thyroid-stimulating hormone level ( J:43657 )

• serum levels of TSH are 2-fold higher in mutants compared to wild-type and heterozygotes

endocrine/exocrine glands

abnormal pituitary gland development ( J:43657 )

• there is a decrease in the number of TSH-positive cells

decreased insulin secretion ( J:43657 )

• in response to 10 and 20 mM glucose, mutant mice show much lower insulin secretion than wild-type mice

nervous system

abnormal pituitary gland development ( J:43657 )

• there is a decrease in the number of TSH-positive cells