Analysis Tools
mortality/aging
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• homozygous embryos are present at the blastocyst stage but absent by E8.5
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Allele Symbol Allele Name Allele ID |
Bub3tm1Jvd targeted mutation 1, Jan M A van Deursen MGI:3619247 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• homozygous embryos are present at the blastocyst stage but absent by E8.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in heterozygous MEFs after 5 passages about 21% of metaphases are aneuploid compared to about 9% in wild-type cells and a wider range of nonmodal chromosome numbers is seen
(J:81546)
• at 5 months of age, 9% of splenocytes are aneuploid
(J:81546)
• the number of aneuploid splenocytes increases from 9% at 5 months to 29% at 27 months compared to only 3% in wild-type mice at 35 months
(J:105717)
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• after nocodazole treatment MEFs leave mitosis prematurely without undergoing cell division
(J:81546)
• overexpression of Rae1 can rescue the mitotic checkpoint defect
(J:81546)
• after nocodazole treatment the time for 50% of cells to exit prometaphase arrest is reduced to 3 hours compared to 7.2 hours for wild-type MEFs
(J:105717)
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• lagging chromosomes are seen in 5% of anaphase figures compared to 2% of wild-type figures
(J:81546)
• in splenocytes prematurely separated sister chromatids increase from 0 at 5 months to being present in 10-11% of metaphases at 24-27 months compared to 0 at 27 months and only 4% at 35 months in wild-type
(J:105717)
• however, no chromosome breaks or fusions are seen at 24 months of age
(J:105717)
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• a single application of 50 ul of 0.5% DMBA at P5 results in an increased incidence of lung tumors and more tumors per animal in heterozygotes compared to wild-type mice
• however, no difference in spontaneous tumor incidence is seen compared to wild-type mice
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• a single application of 50 ul of 0.5% DMBA at P5 results in an increased incidence of lung tumors and more tumors per animal in heterozygotes compared to wild-type mice
• however, no difference in spontaneous tumor incidence is seen compared to wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Reduced lifespan, cataract, lordokyphosis and cachexia in Bub3tm1Jvd/Bub3+ Rae1tm1Jvd/Rae1+ mice
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• seen in the second year of life
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• in double heterozygous MEFs after 5 passages aneuploid cells are increased by 32% compared to wild-type cells and a wider range of nonmodal chromosome numbers is seen compared to single heterozygous or wild-type MEFs
(J:81546)
• at 5 months of age, 37% of splenocytes are aneuploid compared to 9% in single heterozygotes
(J:81546)
• the number of aneuploid splenocytes increases from 37% at 5 months to 47% at 24 months compared to only 3% in wild-type mice at 35 months
(J:105717)
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• after nocodazole treatment the time for 50% of cells to exit prometaphase arrest is reduced to 2.2 hours compared to 7.2 hours for wild-type MEFs
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• prematurely separated sister chromatids are seen in 19% and 13% of mitotic figures from MEFs and splenocytes (at 5 months of age), respectively
(J:81546)
• lagging chromosomes are seen in 15% of anaphase figures compared to 5% of single heterozygous and 2% of wild-type figures
(J:81546)
• in splenocytes prematurely separated sister chromatids increase from 14% at 5 months to being present in 24% of metaphases at 24 months compared to 0 at 27 months and only 4% at 35 months in wild-type
(J:105717)
• however, no chromosome breaks or fusions are seen at 24 months of age
(J:105717)
• 47% of anaphases have multiple lagging chromosomes compared to 0% in wild-type MEFs
(J:105717)
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• double heterozygous MEFs grow slower than single heterozygous or wild-type MEFs
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• at passage 5 and 7 increased expression of senescence related genes in MEFs is seen
• at passage 7 but not passage 3, double heterozygous MEFs grow slower than single heterozygous or wild-type MEFs
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• decreased thickness of the subcutaneous adipose tissue
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• at 27 months of age a dramatic decrease in body fat is seen
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• body weight is similar to wild-type at 5 months of age but significantly lower by 24 months of age
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• clear signs of skeletal muscle degeneration and atrophy are seen in mice with lordokyphosis
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• clear signs of skeletal muscle degeneration and atrophy are seen in mice with lordokyphosis
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• lordokyphosis is seen at an earlier age and with increased severity compared to single heterozygous or wild-type mice
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• incidence of cataract formation is increased and the latency is decreased compared to single heterozygotes
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• a single application of 50 ul of 0.5% DMBA at P5 results in an increased incidence of lung tumors and more tumors per animal in double heterozygotes compared to wild-type mice
• however, no difference in spontaneous tumor incidence is seen compared to wild-type mice
|
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• a single application of 50 ul of 0.5% DMBA at P5 results in an increased incidence of lung tumors and more tumors per animal in double heterozygotes compared to wild-type mice
• however, no difference in spontaneous tumor incidence is seen compared to wild-type mice
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• decreased thickness of the subcutaneous adipose tissue
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• from 5 to 27 months of age dermal thickness decreases by 21% lower compared a decrease of 9% in wild-type mice
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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