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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Aqp8tm1Ask
targeted mutation 1, Alan S Verkman
MGI:3613030
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Aqp8tm1Ask/Aqp8tm1Ask involves: 129/Sv * C57BL/6 * CD-1 MGI:3613464
hm2
 		Aqp8tm1Ask/Aqp8tm1Ask involves: C57BL/6 MGI:7262850
cx3
 		Aqp5tm1Ask/Aqp5tm1Ask
Aqp8tm1Ask/Aqp8tm1Ask 		involves: 129/Sv * C57BL/6 * CD-1 MGI:3613468
cx4
 		Aqp1tm1Ask/Aqp1tm1Ask
Aqp8tm1Ask/Aqp8tm1Ask 		involves: 129/Sv * C57BL/6 * CD-1 MGI:3613472


Genotype
MGI:3613464
hm1
Allelic
Composition		 Aqp8tm1Ask/Aqp8tm1Ask
Genetic
Background		 involves: 129/Sv * C57BL/6 * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aqp8tm1Ask mutation (0 available); any Aqp8 mutation (14 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
homeostasis/metabolism
homeostasis/metabolism phenotype ( J:104681 )
N
• no abnormalities in urine concentrating abilities both before and after water deprivation, in serum chemistry, in cholera toxin-induced fluid secretion, and in osmotically driven water secretion and isosmolar fluid absorption in the small intestine and colon
increased circulating cholesterol level ( J:104681 )
• modest elevation in plasma cholesterol levels when fed a high-fat diet
increased circulating triglyceride level ( J:104681 )
• modest elevation in triglyceride levels when fed a high-fat diet
abnormal fluid regulation ( J:104681 )
• reduction of water permeability in plasma membranes of testes but not the liver

reproductive system
reproductive system phenotype ( J:104681 )
N
• despite testis enlargement, male show no abnormalities in sperm count, sperm morphology, seminiferous tubule size or fertility
enlarged testis ( J:104681 )
• significantly greater size of testes
increased testis weight ( J:104681 )
• significantly greater weight of testes
abnormal spermatogenesis ( J:104681 )
• elevated ratio of spermatogenic cells to Sertoli cells in seminiferous tubules

endocrine/exocrine glands
endocrine/exocrine gland phenotype ( J:104681 )
N
• exhibit no impairment of maximal agonist-stimulated saliva secretion
enlarged testis ( J:104681 )
• significantly greater size of testes
increased testis weight ( J:104681 )
• significantly greater weight of testes




Genotype
MGI:7262850
hm2
Allelic
Composition		 Aqp8tm1Ask/Aqp8tm1Ask
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aqp8tm1Ask mutation (0 available); any Aqp8 mutation (14 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:323121 )
N
• males exhibit normal fertility
decreased granulosa cell apoptosis ( J:323121 )
• uninduced granulosa cells show a significantly lower rate of baseline apoptosis than wild-type granulosa cells
• also, paclitaxel-induced granulosa cells show a significantly lower apoptosis rate than similarly induced wild-type granulosa cells
increased corpora lutea number ( J:323121 )
• at 10 and 20 weeks of age, mature ovaries show a significantly higher number of corpora lutea than wild-type ovaries
abnormal ovarian folliculogenesis ( J:323121 )
• enhanced female fertility is due to increased ovulation associated with altered granulosa cell function and follicular development
• decreased granulosa cell apoptosis probably leads to decreased atresia of growing follicles, followed by increased maturation of pre-ovulatory follicles
abnormal ovulation ( J:323121 )
• during normal ovulation, oocytes harvested from all females appear as cumulus cell-oocyte complexes (COCs); in contrast, oocytes harvested from only 43% wild-type females appear as COCs
increased ovulation rate ( J:323121 )
• in normal ovulation, ovaries from 10-week-old females release significantly more oocytes than wild-type ovaries (9.5 +/- 1.9 versus 7.1 +/- 2.1)
increased superovulation rate ( J:323121 )
• after gonadotropin-induced superovulation, ovaries from 10-week-old females release significantly more oocytes than superovulated wild-type ovaries (37.8 +/- 6.7 versus 27.9 +/- 5.7)
• during superovulation, all oocytes released appear as COCs, similar to oocytes released from wild-type females
increased litter size ( J:323121 )
• 10-week-old females produce a significantly increased litter size regardless of the genotypes of male mates
enhanced female fertility ( J:323121 )
• 10-week-old females exhibit increased fertility, as determined by mating experiments, ovulation studies and ovary histological analysis

cellular
decreased granulosa cell apoptosis ( J:323121 )
• uninduced granulosa cells show a significantly lower rate of baseline apoptosis than wild-type granulosa cells
• also, paclitaxel-induced granulosa cells show a significantly lower apoptosis rate than similarly induced wild-type granulosa cells

homeostasis/metabolism
abnormal fluid regulation ( J:323121 )
• isolated granulosa cells show a ~45% reduction in plasma membrane water permeability relative to wild-type granulosa cells

endocrine/exocrine glands
decreased granulosa cell apoptosis ( J:323121 )
• uninduced granulosa cells show a significantly lower rate of baseline apoptosis than wild-type granulosa cells
• also, paclitaxel-induced granulosa cells show a significantly lower apoptosis rate than similarly induced wild-type granulosa cells
increased corpora lutea number ( J:323121 )
• at 10 and 20 weeks of age, mature ovaries show a significantly higher number of corpora lutea than wild-type ovaries
abnormal ovarian folliculogenesis ( J:323121 )
• enhanced female fertility is due to increased ovulation associated with altered granulosa cell function and follicular development
• decreased granulosa cell apoptosis probably leads to decreased atresia of growing follicles, followed by increased maturation of pre-ovulatory follicles




Genotype
MGI:3613468
cx3
Allelic
Composition		 Aqp5tm1Ask/Aqp5tm1Ask
Aqp8tm1Ask/Aqp8tm1Ask
Genetic
Background		 involves: 129/Sv * C57BL/6 * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aqp5tm1Ask mutation (0 available); any Aqp5 mutation (16 available)
Aqp8tm1Ask mutation (0 available); any Aqp8 mutation (14 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
decreased salivation ( J:104681 )
• saliva secretion is reduced to the same extent as in single homozygous Aqp5 mice

endocrine/exocrine glands
decreased salivation ( J:104681 )
• saliva secretion is reduced to the same extent as in single homozygous Aqp5 mice

homeostasis/metabolism
decreased salivation ( J:104681 )
• saliva secretion is reduced to the same extent as in single homozygous Aqp5 mice




Genotype
MGI:3613472
cx4
Allelic
Composition		 Aqp1tm1Ask/Aqp1tm1Ask
Aqp8tm1Ask/Aqp8tm1Ask
Genetic
Background		 involves: 129/Sv * C57BL/6 * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aqp1tm1Ask mutation (0 available); any Aqp1 mutation (21 available)
Aqp8tm1Ask mutation (0 available); any Aqp8 mutation (14 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
homeostasis/metabolism
decreased urine osmolality ( J:104681 )
• similar urinary hypoosmolality after water deprivation as single homozygous Aqp1 mice

renal/urinary system
decreased urine osmolality ( J:104681 )
• similar urinary hypoosmolality after water deprivation as single homozygous Aqp1 mice




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory