Analysis Tools
immune system
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• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG are completely protected from developing diabetes
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Allele Symbol Allele Name Allele ID |
Tg(Ins2-TFRC/OVA)296Wehi transgene insertion 296, Walter and Eliza Hall Institute of Medical Research MGI:3578729 |
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| Summary |
9 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG are completely protected from developing diabetes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• negative selection of CD 4 T cells bearing the transgenic TCR is impaired as evidenced by only a slight reduction of clonotypic T cells occuring in the thymus and spleen
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• there is a slight reduction in the number of CD4 single positive thymocytes in the thymus compared to controls
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• negative selection of CD 4 T cells bearing the transgenic TCR is impaired as evidenced by only a slight reduction of clonotypic T cells occuring in the thymus and spleen
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• there is a slight reduction in the number of CD4 single positive thymocytes in the thymus compared to controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence of anti-ovalbumin IgG is abolished compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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• only 40% of mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG exhibit diabetes compared to 100% of similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
• the severity of diabetes induced by Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG is reduced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence of anti-ovalbumin IgG is abolished compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence of anti-ovalbumin IgG is abolished compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• the percentage of double-positive T cells in the thymus is increased by 20 % compared to control mice
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• there is a 10 fold drop in the number of CD4 single positive thymocytes in the thymus compared to controls due to increased negative selection of T cell bearing the transgenic TCR
• the number of CD4 T cells in the periphery that express the gene segments associated with the transgenic TCR is reduced almost by half compared to mice carrying just the Tg(TcraTcrb)425Cbn OT-II transgene
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• the percentage of double-positive T cells in the thymus is increased by 20 % compared to control mice
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• there is a 10 fold drop in the number of CD4 single positive thymocytes in the thymus compared to controls due to increased negative selection of T cell bearing the transgenic TCR
• the number of CD4 T cells in the periphery that express the gene segments associated with the transgenic TCR is reduced almost by half compared to mice carrying just the Tg(TcraTcrb)425Cbn OT-II transgene
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• negative selection fails to occur of CD 4 T cells bearing the transgenic TCR as evidenced by the normal number of clonotypic T cells found in the thymus and spleen
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• negative selection fails to occur of CD 4 T cells bearing the transgenic TCR as evidenced by the normal number of clonotypic T cells found in the thymus and spleen
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice injected with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG survive unlike of similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence or absence of anti-ovalbumin IgG is enhanced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse alone exhibit higher a incidence of insulitis than of similarly treated Tg(Ins2-TFRC/OVA)296Wehi and Tg(Ins2-TFRC/OVA)296Wehi Fcer1gtm1Rav/Fcer1gtm1Rav mice
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• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse without anti-ovalbumin IgG exhibit an IFN-gamma production that is increased 3-fold compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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• only 40% of mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG exhibit diabetes compared to 100% of similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
• the severity of diabetes induced by Tg(TcraTcrb)1100Mjb CD8+ T cells and anti-ovalbumin IgG is reduced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse alone exhibit higher a incidence of insulitis than of similarly treated Tg(Ins2-TFRC/OVA)296Wehi and Tg(Ins2-TFRC/OVA)296Wehi Fcer1gtm1Rav/Fcer1gtm1Rav mice
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• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence or absence of anti-ovalbumin IgG is enhanced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in the presence or absence of anti-ovalbumin IgG is enhanced compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG at 10-fold lower doses than in Tg(Ins2-TFRC/OVA)296Wehi mice develop diabetes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse is absent in pancreatic lymph nodes of dendritic cell depleted mice following treatment with diphtheria toxin regardless of whether mice receive anti-ovalbumin IgG
• however, transfer of dendritic cells into depleted mice restores proliferation
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• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse is absent in pancreatic lymph nodes of dendritic cell depleted mice following treatment with diphtheria toxin regardless of whether mice receive anti-ovalbumin IgG
• however, transfer of dendritic cells into depleted mice restores proliferation
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• proliferation of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse is absent in pancreatic lymph nodes of dendritic cell depleted mice following treatment with diphtheria toxin regardless of whether mice receive anti-ovalbumin IgG
• however, transfer of dendritic cells into depleted mice restores proliferation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 75% of mice injected with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG die by day 47
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• injection of OVA-specific CD8+ T cell from OT-1 mice into transgenic female mice results in a 2- to 6-fold higher proportion of OT-1 CD8+ T cells/total CD8+ T cells in the kidney and pancreatic lymph nodes compared with mesenteric, inguinal or cervical lymph nodes or the spleen, while no accumulation of OT-1 CD8+ T cells is seen in non transgenic animals
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• 3 days following injection of OT-1 cells into transgenic animal, OVA-specific CD*+ T cells show activation and 50% of the cells have proliferated in a BrdU assay; injected non-transgenic animals show considerably less activation or proliferation
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• mice treated with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse without anti-ovalbumin IgG exhibit an IFN-gamma production that is decreased 3-fold compared to in similarly treated Tg(Ins2-TFRC/OVA)296Wehi C3tm1Crr/C3tm1Crrmice
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• mice injected with Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse and anti-ovalbumin IgG exhibit increased incidence of diabetes compared to mice receiving Tg(TcraTcrb)1100Mjb CD8+ T cells alone
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• injection of OVA-specific CD8+ T cell from OT-1 mice into transgenic female mice results in a 2- to 6-fold higher proportion of OT-1 CD8+ T cells/total CD8+ T cells in the kidney and pancreatic lymph nodes compared with mesenteric, inguinal or cervical lymph nodes or the spleen, while no accumulation of OT-1 CD8+ T cells is seen in non transgenic animals
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• 3 days following injection of OT-1 cells into transgenic animal, OVA-specific CD*+ T cells show activation and 50% of the cells have proliferated in a BrdU assay; injected non-transgenic animals show considerably less activation or proliferation
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• injection of OVA-specific CD8+ T cell from OT-1 mice into transgenic female mice results in a 2- to 6-fold higher proportion of OT-1 CD8+ T cells/total CD8+ T cells in the kidney and pancreatic lymph nodes compared with mesenteric, inguinal or cervical lymph nodes or the spleen, while no accumulation of OT-1 CD8+ T cells is seen in non transgenic animals
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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