Phenotypes associated with this allele

• Allele Symbol: Klf10^tm1Sub
• Allele Name: targeted mutation 1, Malayannan Subramaniam
• Allele ID: MGI:3530069
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Klf10^tm1Sub/Klf10^tm1Sub	involves: 129 * C57BL/6	MGI:3835938
hm2	Klf10^tm1Sub/Klf10^tm1Sub	involves: C57BL/6	MGI:3530481

Genotype
MGI:3835938

hm1

• Allelic Composition: Klf10^tm1Sub/Klf10^tm1Sub
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

decreased femoral compact bone area ( J:145678 )

• cortical area of the femoral diaphysis is reduced by 21.0% compared to age-matched controls

abnormal tibia morphology ( J:145678 )

• total area of the tibia diaphysis is reduced by 5.0% compared to age-matched controls

• total density of the tibia diaphysis is reduced by 5.3%

• bone mineral content of the tibia diaphysis is reduced by 9.9%

• cortical content of the tibia diaphysis is reduced by 11.2%, cortical area by 10.7% and cortical thickness is reduced by 6.3%

• similar results are observed for the tibia metaphysis

decreased diameter of tibia ( J:145678 )

• periosteal circumference is reduced by 5.2% compared to controls and the endocortical circumference is reduced by 4.9%

decreased bone mineral content ( J:145678 )

• bone mineral content of the tibia diaphysis is reduced by 9.9%

decreased bone mineral density ( J:145678 )

• total density of the tibia diaphysis is reduced by 5.3%

decreased compact bone thickness ( J:145678 )

• the thickness of the femoral diaphysis is reduced by 18.7% compared to age-matched controls

decreased osteocyte number ( J:145678 )

• osteocyte numbers are reduced by a third in the cortical region of the femur

abnormal trabecular bone morphology ( J:145678 )

• surface of trabecular bone within femurs is reduced by more than half compared to controls

decreased trabecular bone volume ( J:145678 )

• there is a 20.4% reduction in the trabecular bone volume to total bone volume ratio within the femoral head of 3-month old mice

abnormal bone trabecula morphology ( J:145678 )

• the trabecular spacing in the femoral heads is increased by 13%

decreased bone strength ( J:145678 )

• mean force to break a 3-month old femur is 26.1% less than controls and the work to failure is 22.3% less than controls

• however, stiffness was normal in the femurs

limbs/digits/tail

decreased femoral compact bone area ( J:145678 )

• cortical area of the femoral diaphysis is reduced by 21.0% compared to age-matched controls

abnormal tibia morphology ( J:145678 )

• total area of the tibia diaphysis is reduced by 5.0% compared to age-matched controls

• total density of the tibia diaphysis is reduced by 5.3%

• bone mineral content of the tibia diaphysis is reduced by 9.9%

• cortical content of the tibia diaphysis is reduced by 11.2%, cortical area by 10.7% and cortical thickness is reduced by 6.3%

• similar results are observed for the tibia metaphysis

decreased diameter of tibia ( J:145678 )

• periosteal circumference is reduced by 5.2% compared to controls and the endocortical circumference is reduced by 4.9%

Genotype
MGI:3530481

hm2

• Allelic Composition: Klf10^tm1Sub/Klf10^tm1Sub
• Genetic Background: involves: C57BL/6

skeleton

increased osteoblast cell number ( J:95992 )

• the number of osteoblast, but not osteoclasts, is increased; however, bone formation is not increased

abnormal bone ossification ( J:95992 )

• cultured mutant osteoblasts lack the capacity to form mineralized nodules and are impaired in their ability to support osteoclast differentiation