Phenotypes associated with this allele

• Allele Symbol: Il21^tm1Lex
• Allele Name: targeted mutation 1, Lexicon Genetics
• Allele ID: MGI:3528994
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Il21^tm1Lex/Il21^tm1Lex	involves: 129S5/SvEvBrd * C57BL/6	MGI:3814049
hm2	Il21^tm1Lex/Il21^tm1Lex	involves: 129S5/SvEvBrd * C57BL/6J	MGI:3609970

Genotype
MGI:3814049

hm1

• Allelic Composition: Il21^tm1Lex/Il21^tm1Lex
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased germinal center B cell number ( J:137867 )

• the percentage of GC B cells that make up the total B cell population in the spleen of immunized mice is reduced by two-thirds

abnormal CD4-positive, alpha beta T cell morphology ( J:137867 )

• the percentage of follicular T helper cells (defined as CXCR5⁺ICOS⁺CD4⁺ T cells) is reduced by a third

abnormal spleen germinal center morphology ( J:137867 )

• the number of actively proliferating T cells found within GC is reduced 3- to 4- fold

• the percentage of follicular T helper cells (defined as CXCR5⁺ICOS⁺CD4⁺ T cells) is reduced by a third

decreased spleen germinal center number ( J:137867 )

• there is a paucity of GC in the spleen of mice after immunization with sheep red blood cells (SRBC)

abnormal germinal center B cell physiology ( J:137867 )

• the number of germinal center B cells that are actively proliferating is reduced 5-fold

decreased IgG1 level ( J:137867 )

• serum IgG1 after immunization with a T cell dependent antigen is reduced by more than half compared to controls

abnormal CD4-positive, alpha-beta T cell physiology ( J:137867 )

• transfer studies suggest that an intrinsic defect of follicular T helper cells leads to improper activation of B cells and reduced IgG1 levels to T cell dependent antigens

immune system

decreased germinal center B cell number ( J:137867 )

• the percentage of GC B cells that make up the total B cell population in the spleen of immunized mice is reduced by two-thirds

abnormal CD4-positive, alpha beta T cell morphology ( J:137867 )

• the percentage of follicular T helper cells (defined as CXCR5⁺ICOS⁺CD4⁺ T cells) is reduced by a third

abnormal spleen germinal center morphology ( J:137867 )

• the number of actively proliferating T cells found within GC is reduced 3- to 4- fold

• the percentage of follicular T helper cells (defined as CXCR5⁺ICOS⁺CD4⁺ T cells) is reduced by a third

decreased spleen germinal center number ( J:137867 )

• there is a paucity of GC in the spleen of mice after immunization with sheep red blood cells (SRBC)

abnormal germinal center B cell physiology ( J:137867 )

• the number of germinal center B cells that are actively proliferating is reduced 5-fold

decreased IgG1 level ( J:137867 )

• serum IgG1 after immunization with a T cell dependent antigen is reduced by more than half compared to controls

abnormal CD4-positive, alpha-beta T cell physiology ( J:137867 )

• transfer studies suggest that an intrinsic defect of follicular T helper cells leads to improper activation of B cells and reduced IgG1 levels to T cell dependent antigens

Genotype
MGI:3609970

hm2

• Allelic Composition: Il21^tm1Lex/Il21^tm1Lex
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J

immune system

abnormal T cell differentiation ( J:123351 )

• when naive T_H cells are subjected to T_H17 differentiation, a deficit in generation of Il-17-producing cells is observed with an increased number of Foxp3+ cells compared to wild-type

• one week after immunization with MOG peptide in CFA, restimulation of spleen cells with TPA or MOG peptide results in production of almost no Il-17 producing cells, showing impaired T_H17 differentiation

decreased CD4-positive, alpha-beta T cell number ( J:123351 )

• Il-17+ CD4+ cells are absent from lamina propria and spleen in mutants

abnormal gamma-delta T cell morphology ( J:123351 )

• lamina propria and splenic TCRgd+ T cells show <10-fold decrease in Il-17-expressing cells compared to wild-type cells

abnormal interleukin level ( J:123351 )

• CNS-infiltrating and splenic CD4+ T cells produce interferon gamma, in contrast to CD4+ T cells from controls which produce predominantly Il-17

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:123351 )

• after second immunization with MOG peptide, mutants show signs of EAE by 8 days and only mild disease is observed after 11 days, whereas wild-type mice show disease after 5 days with increased severity by 11 days

increased susceptibility to experimental autoimmune encephalomyelitis ( J:137377 )

• increased susceptibility when injected with myelin oligodendrocyte glycoprotein

hematopoietic system

abnormal T cell differentiation ( J:123351 )

• when naive T_H cells are subjected to T_H17 differentiation, a deficit in generation of Il-17-producing cells is observed with an increased number of Foxp3+ cells compared to wild-type

• one week after immunization with MOG peptide in CFA, restimulation of spleen cells with TPA or MOG peptide results in production of almost no Il-17 producing cells, showing impaired T_H17 differentiation

decreased CD4-positive, alpha-beta T cell number ( J:123351 )

• Il-17+ CD4+ cells are absent from lamina propria and spleen in mutants

abnormal gamma-delta T cell morphology ( J:123351 )

• lamina propria and splenic TCRgd+ T cells show <10-fold decrease in Il-17-expressing cells compared to wild-type cells

homeostasis/metabolism

abnormal interleukin level ( J:123351 )

• CNS-infiltrating and splenic CD4+ T cells produce interferon gamma, in contrast to CD4+ T cells from controls which produce predominantly Il-17