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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Thtm5Rpa
targeted mutation 5, Richard D Palmiter
MGI:3527166
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Thtm5Rpa/Thtm5Rpa involves: 129S4/SvJaeSor * C57BL/6 MGI:3711316
cn2
Dbhtm2(Th)Rpa/Dbh+
Thtm5Rpa/Thtm5Rpa
involves: 129S4/SvJaeSor * C57BL/6 MGI:3654637
cx3
Dbhtm2(Th)Rpa/Dbh+
Thtm5Rpa/Thtm5Rpa
involves: 129S4/SvJaeSor MGI:4414756
cx4
Dbhtm2(Th)Rpa/Dbh+
Thtm5Rpa/Thtm5Rpa
involves: 129S4/SvJaeSor * C57BL/6 MGI:3654638


Genotype
MGI:3711316
hm1
Allelic
Composition
Thtm5Rpa/Thtm5Rpa
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Thtm5Rpa mutation (0 available); any Th mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no homozygotes are found at birth




Genotype
MGI:3654637
cn2
Allelic
Composition
Dbhtm2(Th)Rpa/Dbh+
Thtm5Rpa/Thtm5Rpa
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dbhtm2(Th)Rpa mutation (1 available); any Dbh mutation (7 available)
Thtm5Rpa mutation (0 available); any Th mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• bilateral injection of CAVCre (cre recombinase delivered by a canine adenovirus vector, efficient uptake into dopaminergic neurons) into the central region of the caudate putamen allows mice to survive without L-DOPA treatment

behavior/neurological
• after viral Cre treatment and a 24 hour fast, mice consume less food when a standard chow diet is provided but a similar amount, compared to wild-type controls, when a higher-fat breeder chow diet is provided
• for the first 4 days after viral Cre treatment mice consume more than wild-type controls
• after viral Cre treatment on a highly palatable liquid diet mice consume large meals and tend to lick faster than wild-type controls
• seen in an open field test after viral Cre treatment
• however, other measures of anxiety (time spent in open arms of a maze and entry into open arms) are similar to controls
• after viral Cre treatment performance on a rotarod improves with practice but is consistently worse than in wild-type controls
• hyperactive after viral Cre treatment
• L-DOPA treatment increased activity in both Cre treated and untreated mice but not in wild-type controls
• however, exploratory locomotor response is similar to controls

nervous system
• after viral cre treatment dopamine levels in the brain to about 55% of controls and dopamine metabolite levels are also partially restores
• evoked dopamine release in the sections from the caudate putamen is about 30% of controls

growth/size/body
• prior to viral cre treatment and for 1 - 3 months after viral cre treatment mice weigh less than controls
• after viral cre treatment, mice gain weight more rapidly than controls reaching body weights similar to controls by 1 - 3 months after treatment

homeostasis/metabolism
• after viral cre treatment dopamine levels in the brain to about 55% of controls and dopamine metabolite levels are also partially restores
• evoked dopamine release in the sections from the caudate putamen is about 30% of controls




Genotype
MGI:4414756
cx3
Allelic
Composition
Dbhtm2(Th)Rpa/Dbh+
Thtm5Rpa/Thtm5Rpa
Genetic
Background
involves: 129S4/SvJaeSor
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dbhtm2(Th)Rpa mutation (1 available); any Dbh mutation (7 available)
Thtm5Rpa mutation (0 available); any Th mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• conditioned place aversion to the KOR agonist U50,488 is slightly but significantly increased compared to littermate controls

nervous system

homeostasis/metabolism




Genotype
MGI:3654638
cx4
Allelic
Composition
Dbhtm2(Th)Rpa/Dbh+
Thtm5Rpa/Thtm5Rpa
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dbhtm2(Th)Rpa mutation (1 available); any Dbh mutation (7 available)
Thtm5Rpa mutation (0 available); any Th mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• without viral cre injection, phenotype is stated to be indistinguishable from mice homozygous for Thtm1Rpa and heterozygous for Dbhtm2(Th)Rpa
• mice die within 3 days of stopping L-DOPA treatment

behavior/neurological
• in the absence of L-DOPA treatment
• severely hypoactive

nervous system
• dopamine levels are 0.56% of controls
• dopamine metabolite levels are also reduced
• evoked dopamine release in caudate putamen sections is absent

growth/size/body
• cessation of L-DOPA treatment results in weight loss

homeostasis/metabolism
• dopamine levels are 0.56% of controls
• dopamine metabolite levels are also reduced
• evoked dopamine release in caudate putamen sections is absent





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last database update
03/13/2019
MGI 6.13
The Jackson Laboratory