Analysis Tools
mortality/aging
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• no homozygotes are found at birth
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Allele Symbol Allele Name Allele ID |
Thtm5Rpa targeted mutation 5, Richard D Palmiter MGI:3527166 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no homozygotes are found at birth
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• bilateral injection of CAVCre (cre recombinase delivered by a canine adenovirus vector, efficient uptake into dopaminergic neurons) into the central region of the caudate putamen allows mice to survive without L-DOPA treatment
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• after viral Cre treatment and a 24 hour fast, mice consume less food when a standard chow diet is provided but a similar amount, compared to wild-type controls, when a higher-fat breeder chow diet is provided
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• for the first 4 days after viral Cre treatment mice consume more than wild-type controls
• after viral Cre treatment on a highly palatable liquid diet mice consume large meals and tend to lick faster than wild-type controls
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• seen in an open field test after viral Cre treatment
• however, other measures of anxiety (time spent in open arms of a maze and entry into open arms) are similar to controls
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• after viral Cre treatment performance on a rotarod improves with practice but is consistently worse than in wild-type controls
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• hyperactive after viral Cre treatment
• L-DOPA treatment increased activity in both Cre treated and untreated mice but not in wild-type controls
• however, exploratory locomotor response is similar to controls
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• prior to viral cre treatment and for 1 - 3 months after viral cre treatment mice weigh less than controls
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• after viral cre treatment, mice gain weight more rapidly than controls reaching body weights similar to controls by 1 - 3 months after treatment
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• after viral cre treatment dopamine levels in the brain to about 55% of controls and dopamine metabolite levels are also partially restores
• evoked dopamine release in the sections from the caudate putamen is about 30% of controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• conditioned place aversion to the KOR agonist U50,488 is slightly but significantly increased compared to littermate controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• without viral cre injection, phenotype is stated to be indistinguishable from mice homozygous for Thtm1Rpa and heterozygous for Dbhtm2(Th)Rpa
• mice die within 3 days of stopping L-DOPA treatment
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• severely hypoactive
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• cessation of L-DOPA treatment results in weight loss
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• dopamine levels are 0.56% of controls
• dopamine metabolite levels are also reduced
• evoked dopamine release in caudate putamen sections is absent
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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