Mouse Genome Informatics
hm1
    Syngap1tm1.1Mabk/Syngap1tm1.1Mabk
involves: 129S1/Sv * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• mice generally died on P1 or by early P2

behavior/neurological
• displayed trembling by P1
• mice begin to weaken at P1

growth/size
• mice do not grow well and, by P1, become smaller than wild-type and develop a "failure-to-thrive" phenotype

nervous system
• many more neurons stain positively for activated caspase-3 in the brains of homozygous mice at P1
• the differences can be seen in the hippocampus at E18 and is also evident at P0
• in the hippocampus, most of these apoptotic neurons are found in stratum oriens and the pyramidal layer
• spine maturation of hippocampal neurons cultured in vitro for 10 days from E16-E17 embryos occurred earlier than in wild-type neurons, with dendrites exhibiting an increase in the number of protrusions
• neurons cultured in vitro for 9 days exhibited a higher frequency and larger amplitude of miniature EPSC than wild-type neurons

cellular
• many more neurons stain positively for activated caspase-3 in the brains of homozygous mice at P1
• the differences can be seen in the hippocampus at E18 and is also evident at P0
• in the hippocampus, most of these apoptotic neurons are found in stratum oriens and the pyramidal layer


Mouse Genome Informatics
cn2
    Syngap1tm1.1Mabk/Syngap1tm1.2Mabk
Tg(Camk2a-cre)2834Lusc/0

involves: 129S1/Sv * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• 12.6% (13 out of 103) referred to as "mini" were significantly smaller at P7 and died by 3 weeks after birth
• remaining mutant were phenotypically normal up to the forth postnatal week and their weight was statistically indistinguishable from controls

nervous system
• many more neurons stain positively for activated caspase-3 in the brains of homozygous mice at 2 weeks and 8weeks after birth
• more activation of caspase-3 in the cell body layers of the hippocampus were found note: level of synGAP protein correspond to 20-25% of wild-type in ""mini"" group and 37.5% of wild-type in normal group more activation of caspase-3 in the cell body layerof the hippocampus were found note: level of synGAP protein correspond to 20-25% of wild-type in ""mini"" group and 37.5% of wild-type in normal group more activation of caspase-3 in the cell body layers of the hippocampus were found note: level of synGAProtein correspond to 20-25% of wild-type in ""mini"" group and 37.5% of wild-type in normal group more activation of caspase-3 in the cell body layers of the hippocampus were found
• level of synGAP protein correspond to 20-25% of wild-type in ""mini"" group and 37.5% of wild-type in normal group
• level of synGAP protein correlate with severity of neuronal apoptosis

cellular
• many more neurons stain positively for activated caspase-3 in the brains of homozygous mice at 2 weeks and 8weeks after birth
• more activation of caspase-3 in the cell body layers of the hippocampus were found note: level of synGAP protein correspond to 20-25% of wild-type in ""mini"" group and 37.5% of wild-type in normal group more activation of caspase-3 in the cell body layerof the hippocampus were found note: level of synGAP protein correspond to 20-25% of wild-type in ""mini"" group and 37.5% of wild-type in normal group more activation of caspase-3 in the cell body layers of the hippocampus were found note: level of synGAProtein correspond to 20-25% of wild-type in ""mini"" group and 37.5% of wild-type in normal group more activation of caspase-3 in the cell body layers of the hippocampus were found
• level of synGAP protein correspond to 20-25% of wild-type in ""mini"" group and 37.5% of wild-type in normal group
• level of synGAP protein correlate with severity of neuronal apoptosis