Phenotypes associated with this allele

• Allele Symbol: Bub1b^tm1.1Jvd
• Allele Name: targeted mutation 1.1, Jan M A van Deursen
• Allele ID: MGI:3046581
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Bub1b^tm1.1Jvd/Bub1b^tm1.1Jvd	involves: 129S6/SvEvTac	MGI:3046585
ht2	Bub1b^tm1.1Jvd/Bub1b^+	involves: 129S6/SvEvTac	MGI:3620042
ht3	Bub1b^tm1.1Jvd/Bub1b^+	involves: 129S6/SvEvTac * C57BL/6	MGI:6478927
ht4	Bub1b^tm1Jvd/Bub1b^tm1.1Jvd	involves: 129S6/SvEvTac	MGI:3046584
ht5	Bub1b^tm1Jvd/Bub1b^tm1.1Jvd	involves: 129S6/SvEvTac * C57BL/6	MGI:6478930

Genotype
MGI:3046585

hm1

• Allelic Composition: Bub1b^tm1.1Jvd/Bub1b^tm1.1Jvd
• Genetic Background: involves: 129S6/SvEvTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:91128 )

• early embryonic lethality

Genotype
MGI:3620042

ht2

• Allelic Composition: Bub1b^tm1.1Jvd/Bub1b⁺
• Genetic Background: involves: 129S6/SvEvTac

mortality/aging

premature death ( J:194926 )

• reduced median (90 vs. 102 weeks) without a reduction in maximum lifespan

cellular

abnormal cell cycle checkpoint function ( J:105717 )

• after nocodazole treatment the time for 50% of cells to exit prometaphase arrest is reduced to 3.8 hours compared to 7.2 hours for wild-type MEFs

Genotype
MGI:6478927

ht3

• Allelic Composition: Bub1b^tm1.1Jvd/Bub1b⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

mortality/aging

premature death ( J:293084 )

• mice have a modest, but significant, reduction in lifespan

• however, mice show no evidence of accelerated aging phenotypes

cellular

aneuploidy ( J:293084 )

• MEFs show an elevation in aneuploidy

abnormal mitosis ( J:293084 )

• MEFs exhibit premature chromatid separation

• MEFs exhibit small, but significant, increase in chromosome segregation errors; both spindle assembly checkpoint and attachment error correction machinery are impaired

abnormal mitotic spindle assembly checkpoint ( J:293084 )

• spindle assembly checkpoint is impaired in MEFs

neoplasm

neoplasm ( J:293084 )

N • incidence and spectrum of spontaneous tumors are similar to wild-type

increased tumor growth/size ( J:293084 )

• DMBA-treated mice show no increase in lung tumor incidence, however, lung tumor multiplicity and size are increased

decreased tumor latency ( J:293084 )

• although incidence and spectrum of spontaneous tumors are similar to wild-type, tumor latencies are reduced (741 days versus 886 days in wild-type mice)

• lymphomas which develop with reduced latencies contain more mitotic cells than lymphomas from wild-type mice

Genotype
MGI:3046584

ht4

• Allelic Composition: Bub1b^tm1Jvd/Bub1b^tm1.1Jvd
• Genetic Background: involves: 129S6/SvEvTac

mortality/aging

neonatal lethality, complete penetrance ( J:91128 )

• mutants die within a few hours of birth apparently from respiratory insufficiency

Genotype
MGI:6478930

ht5

• Allelic Composition: Bub1b^tm1Jvd/Bub1b^tm1.1Jvd
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

mortality/aging

neonatal lethality, complete penetrance ( J:293084 )

• mice fail to thrive and die within 18 hours after birth