Phenotypes associated with this allele

• Allele Symbol: Cx3cr1^tm1Ifc
• Allele Name: targeted mutation 1, Israel F Charo
• Allele ID: MGI:2450885
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cx3cr1^tm1Ifc/Cx3cr1^tm1Ifc	involves: 129S4/SvJae * C57BL/6	MGI:2450890
cx2	Apoe^tm1Bres/Apoe^tm1Bres Cx3cr1^tm1Ifc/Cx3cr1^tm1Ifc	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6	MGI:2450893

Genotype
MGI:2450890

hm1

• Allelic Composition: Cx3cr1^tm1Ifc/Cx3cr1^tm1Ifc
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:82033 )

N • homozygotes are viable, fertile and exhibit neither developmental defects nor any differences in disease severity in myelin oligodendrocyte glycoprotein-induced (MOG-induced) experimental autoimmune encephalomyelitis (EAE) relative to wild-type mice

N • in response to to antibody-induced glomerulonephritis, homozygotes and wild-type mice exhibit equally severe glomerular and interstitial injury, as shown by comparable elevation of BUN, proteinuria, and renal inflammation

impaired macrophage chemotaxis ( J:82033 )

• at day 3 after cardiac transplantation, CsA-treated mutant allograft recipients display significantly reduced macrophage infiltration into grafted hearts

abnormal NK cell physiology ( J:82033 )

• at day 3 after cardiac transplantation, CsA-treated mutant allograft recipients display significantly reduced NK cell infiltration into grafted hearts

abnormal response to transplant ( J:82033 )

• in the absence of cyclosporin A (CsA), homozygotes reject fully MHC-disparate cardiac allografts at the same tempo as wild-type recipients

• however, in the presence of a subtherapeutic dose of CsA, homozygotes display a dramatic prolongation of graft survival relative to CsA-treated wild-type mice

• at day 3 after cardiac transplantation, wild-type recipients (with or without CsA) and homozygous mutant recipients without CsA show severe cellular rejection with extensive mononuclear cell infiltration and myocyte necrosis, whereas CsA-treated mutant recipients display intact myocardium and vessels and only a mild degree of NK cell and monocyte/macrophage infiltration

• increased survival of CsA-treated homozygotes is associated with reduced chemokine and cytokine levels in the grafted hearts

cellular

impaired macrophage chemotaxis ( J:82033 )

• at day 3 after cardiac transplantation, CsA-treated mutant allograft recipients display significantly reduced macrophage infiltration into grafted hearts

hematopoietic system

impaired macrophage chemotaxis ( J:82033 )

• at day 3 after cardiac transplantation, CsA-treated mutant allograft recipients display significantly reduced macrophage infiltration into grafted hearts

abnormal NK cell physiology ( J:82033 )

• at day 3 after cardiac transplantation, CsA-treated mutant allograft recipients display significantly reduced NK cell infiltration into grafted hearts

Genotype
MGI:2450893

cx2

• Allelic Composition: Apoe^tm1Bres/Apoe^tm1Bres; Cx3cr1^tm1Ifc/Cx3cr1^tm1Ifc
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6

cardiovascular system

decreased susceptibility to atherosclerosis ( J:81822 )

• on a Western-type high-fat diet, double homozygotes show significant reductions in aortic atherosclerotic lesion area and thickness relative to single Apoe ^tm1Bres homozygotes (43%, 49%, and 36% at 5, 10, and 15 weeks, respectively)

• notably, double homozygotes exhibit retarded lesion development in both the aortic arch and thoracic/abdominal sections of the aorta; however, this difference is less pronounced in the arch

immune system

impaired macrophage chemotaxis ( J:81822 )

• after 10 weeks on a Western-type high-fat diet, double homozygotes exhibit a 40% reduction in macrophage infiltration of the aortic sinus relative to single Apoe^tm1Bres homozygotes

cellular

impaired macrophage chemotaxis ( J:81822 )

• after 10 weeks on a Western-type high-fat diet, double homozygotes exhibit a 40% reduction in macrophage infiltration of the aortic sinus relative to single Apoe^tm1Bres homozygotes

hematopoietic system

impaired macrophage chemotaxis ( J:81822 )

• after 10 weeks on a Western-type high-fat diet, double homozygotes exhibit a 40% reduction in macrophage infiltration of the aortic sinus relative to single Apoe^tm1Bres homozygotes