All Phenotypes Nodal<tm2Rob> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Nodal^tm2Rob
targeted mutation 2, Elizabeth J Robertson
MGI:2181583

Summary

7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nodal^tm2Rob/Nodal^tm2Rob	129S(Cg)-Nodal^tm2Rob	MGI:5827600
hm2	Nodal^tm2Rob/Nodal^tm2Rob	involves: 129S/SvEv * ICR	MGI:2183475
ht3	Nodal^tm1Rob/Nodal^tm2Rob	involves: 129S/SvEv * ICR	MGI:3851002
cx4	Nodal^tm2Rob/Nodal⁺ Zic2^Ku/Zic2^Ku	129S.Cg-Nodal^tm2Rob Zic2^Ku	MGI:5827602
cx5	Nodal^tm2Rob/Nodal^tm2Rob Zic2^Ku/Zic2^Ku	129S.Cg-Nodal^tm2Rob Zic2^Ku	MGI:5827603
cx6	Nodal^tm2Rob/Nodal^tm2Rob Zic2^Ku/Zic2⁺	129S.Cg-Nodal^tm2Rob Zic2^Ku	MGI:5827604
cx7	Nodal^tm1Rob/Nodal^tm2Rob Trim33^tm1.2Los/Trim33^tm1.2Los	involves: 129S/SvEv * 129S2/SvPas * C57BL/6	MGI:4830331

Allelic Composition	Nodal^tm2Rob/Nodal^tm2Rob
Genetic Background	129S(Cg)-Nodal^tm2Rob

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodal^tm2Rob mutation (0 available); any Nodal mutation (41 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

abnormal mesendoderm development ( J:238567 )

• axial mesendoderm does not develop

rostral body truncation ( J:238567 )

• embryos show a spectrum of defects, ranging from no phenotype through a moderate anterior truncation in which some of the midbrain is retain to a severe anterior truncation in which all forebrain and midbrain tissue is absent

Allelic Composition	Nodal^tm2Rob/Nodal^tm2Rob
Genetic Background	involves: 129S/SvEv * ICR

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodal^tm2Rob mutation (0 available); any Nodal mutation (41 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:77125 )

• all mice die by 6 weeks of age

prenatal lethality, incomplete penetrance ( J:77125 )

• 30% of mice survive to birth

cardiovascular system

aortic hypertrophy ( J:77125 )

abnormal heart looping ( J:77125 )

• at E9.5, the heart loops ventrally instead of laterally as in wild-type mice

abnormal heart apex morphology ( J:77125 )

• at E11.5, heart apex is ambiguously positioned unlike in wild-type mice

abnormal heart position or orientation ( J:77125 )

• at E9.0, the heart is positioned more ventrally than normal

respiratory system

right pulmonary isomerism ( J:77125 )

• mutant embryos have 4 right lobes and 2 or 3 left lobes, indicating partial right pulmonary isomerism

embryo

embryo phenotype ( J:77125 )

N	• mice exhibit normal gastrulation

growth/size/body

decreased body size ( J:77125 )

• mice are runted

right-sided isomerism ( J:77125 )

• partial

right pulmonary isomerism ( J:77125 )

• mutant embryos have 4 right lobes and 2 or 3 left lobes, indicating partial right pulmonary isomerism

Allelic Composition	Nodal^tm1Rob/Nodal^tm2Rob
Genetic Background	involves: 129S/SvEv * ICR

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodal^tm1Rob mutation (1 available); any Nodal mutation (41 available)
Nodal^tm2Rob mutation (0 available); any Nodal mutation (41 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

abnormal gastrulation ( J:77125 )

• in some mice

abnormal gastrulation movements ( J:77125 )

• severely affected mice exhibit a decrease in midline tissue compared with wild-type mice due to abnormal gastrulation movements

abnormal rostral-caudal axis patterning ( J:77125 )

• at E8.5, mice exhibit a reduction in anterior neural tissues compared with wild-type mice

absent notochord ( J:77125 )

• in some mice

abnormal primitive node morphology ( J:77125 )

• in some mice

fused somites ( J:77125 )

• fused somites

embryonic-extraembryonic boundary constriction ( J:77125 )

• constriction at the boundary in 40% of mice at E6.5

abnormal visceral endoderm morphology ( J:77125 )

• at E6.5, 40% of embryos are abnormal with thickened patch of visceral endoderm characteristic of the anterior visceral endoderm

cardiovascular system

abnormal heart looping ( J:77125 )

• in some mice

Allelic Composition	Nodal^tm2Rob/Nodal⁺ Zic2^Ku/Zic2^Ku
Genetic Background	129S.Cg-Nodal^tm2Rob Zic2^Ku

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodal^tm2Rob mutation (0 available); any Nodal mutation (41 available)
Zic2^Ku mutation (4 available); any Zic2 mutation (41 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

abnormal mesendoderm development ( J:238567 )

• axial mesendoderm loss

rostral body truncation ( J:238567 )

• 38% of embryos present with moderate or severe anterior truncation

Allelic Composition	Nodal^tm2Rob/Nodal^tm2Rob Zic2^Ku/Zic2^Ku
Genetic Background	129S.Cg-Nodal^tm2Rob Zic2^Ku

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

rostral body truncation ( J:238567 )

• 100% of embryos present with moderate or severe anterior truncation

Allelic Composition	Nodal^tm2Rob/Nodal^tm2Rob Zic2^Ku/Zic2⁺
Genetic Background	129S.Cg-Nodal^tm2Rob Zic2^Ku

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

rostral body truncation ( J:238567 )

• 72% of embryos present with moderate to severe anterior truncation

Allelic Composition	Nodal^tm1Rob/Nodal^tm2Rob Trim33^tm1.2Los/Trim33^tm1.2Los
Genetic Background	involves: 129S/SvEv * 129S2/SvPas * C57BL/6

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

embryo phenotype ( J:163858 )

N	• at E6.25 decrease in Nodal expression rescues patterning defects in the anterior visceral endoderm, extraembryonic ectoderm and mesoderm and the decrease in embryo size that are seen in Trim33 single homozygotes

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