Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptprctm1Mak mutation
(2 available);
any
Ptprc mutation
(188 available)
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immune system
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• intestinal intraepithelial lymphocytes (iIELs) exhibit increased spontaneous apoptosis compared to in wild-type mice
• gamma-delta iIELs are more susceptible to induction of apoptosis compared with alpha-beta iIELs
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• intestinal intraepithelial lymphocytes (iIELs) is impaired
• however, extrathymic development of iIELs is normal in reconstitution experiments
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• intestinal intraepithelial lymphocytes (iIELs) are slightly larger than wild-type cells
• in reconstitution experiments, intrathymic development of iIELs is impaired
• however, extrathymic development of iIELs is normal in reconstitution experiments
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• at 10 weeks, the number of intestinal intraepithelial lymphocytes (iIELs) is increased 1.5- to 2-fold compared to in wild-type mice
• at 10 weeks, the number of CD4+CD8- alpha-beta iIELs is increased 7-fold compared to in wild-type mice
• the number of iIELs decrease over time unlike in wild-type mice that exhibit an increase in these cells
• at 20 weeks, iIELs are decreased compared to in wild-type mice
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• at 10 and 20 weeks, mice exhibit decreased gamma-delta intestinal intraepithelial lymphocytes, which continue to decrease in number with age, compared with wild-type mice
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• at 10 weeks, the number of CD4+CD8- alpha-beta intestinal intraepithelial lymphocytes is increased 7-fold compared to in wild-type mice
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• NK cells stimulated with ligands to or antibodies against NK1.1, CD16, Ly49H, Ly49D, and NKG2D exhibit reduced cytokine secretion compared with similarly treated wild-type cells
• however, NK cell cytotoxicity in response to ITAM receptor stimulation is normal
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• intestinal intraepithelial lymphocytes exhibit impaired cytolytic activities compared with wild-type cells
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hematopoietic system
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• intestinal intraepithelial lymphocytes (iIELs) exhibit increased spontaneous apoptosis compared to in wild-type mice
• gamma-delta iIELs are more susceptible to induction of apoptosis compared with alpha-beta iIELs
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• intestinal intraepithelial lymphocytes (iIELs) is impaired
• however, extrathymic development of iIELs is normal in reconstitution experiments
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• intestinal intraepithelial lymphocytes (iIELs) are slightly larger than wild-type cells
• in reconstitution experiments, intrathymic development of iIELs is impaired
• however, extrathymic development of iIELs is normal in reconstitution experiments
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• at 10 weeks, the number of intestinal intraepithelial lymphocytes (iIELs) is increased 1.5- to 2-fold compared to in wild-type mice
• at 10 weeks, the number of CD4+CD8- alpha-beta iIELs is increased 7-fold compared to in wild-type mice
• the number of iIELs decrease over time unlike in wild-type mice that exhibit an increase in these cells
• at 20 weeks, iIELs are decreased compared to in wild-type mice
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• at 10 and 20 weeks, mice exhibit decreased gamma-delta intestinal intraepithelial lymphocytes, which continue to decrease in number with age, compared with wild-type mice
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• at 10 weeks, the number of CD4+CD8- alpha-beta intestinal intraepithelial lymphocytes is increased 7-fold compared to in wild-type mice
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• NK cells stimulated with ligands to or antibodies against NK1.1, CD16, Ly49H, Ly49D, and NKG2D exhibit reduced cytokine secretion compared with similarly treated wild-type cells
• however, NK cell cytotoxicity in response to ITAM receptor stimulation is normal
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• intestinal intraepithelial lymphocytes exhibit impaired cytolytic activities compared with wild-type cells
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cellular
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• intestinal intraepithelial lymphocytes (iIELs) exhibit increased spontaneous apoptosis compared to in wild-type mice
• gamma-delta iIELs are more susceptible to induction of apoptosis compared with alpha-beta iIELs
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptprctm1Mak mutation
(2 available);
any
Ptprc mutation
(188 available)
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immune system
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• IgE monoclonal anti-DNP antibody primed mast cells are incapable of significant degranulation unlike similarly treated wild-type cells
• however, cells exhibit normal
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• osteoclasts exhibit impaired bone remodeling compared with wild-type cells
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• mice are resistant to IgE-mediated anaphylaxis unlike wild-type mice
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nervous system
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• oligodendrocyte precursor cells fail to differentiate in the presence of IgG or anti-Fcrg antibodies unlike wild-type cells
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skeleton
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• osteoclasts exhibit impaired bone remodeling compared with wild-type cells
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• metaphyseal bone trabecules are irregularly distributed compared to in wild-type mice
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hematopoietic system
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• however, cells exhibit normal
• IgE monoclonal anti-DNP antibody primed mast cells are incapable of significant degranulation unlike similarly treated wild-type cells
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• bone marrow leukocytes exhibit defective motility, progenitor cell expansion, homing, and mobilization by colony-stimulating factor (G-CSF) and increased cell adhesion compared with wild-type cells
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• osteoclasts exhibit impaired bone remodeling compared with wild-type cells
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cellular
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• IgE monoclonal anti-DNP antibody primed mast cells are incapable of significant degranulation unlike similarly treated wild-type cells
• however, cells exhibit normal
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptprctm1Mak mutation
(2 available);
any
Ptprc mutation
(188 available)
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immune system
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• in response to anti-mu
• however, LPS activated B cell proliferation is normal
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• following stimulation with lection and TCR-CD3 cross-linking
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• the transition from double positive to single positive is blocked unlike in wild-type mice
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• the ratio of CD8+ to CD4+ T cells is skewed towards CD4+ T cells unlike in wild-type mice
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• in the spleen, lymph nodes, and bone marrow with CD8+ T cells reduced to a greater extent than CD4+ T cells
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• in mice exposed to lymphocytic choriomeningitis virus (LCMV)
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• mice exposed to lymphocytic choriomeningitis virus (LCMV) fail to exhibit a T cell cytotoxic response unlike similarly treated wild-type mice
• however, mice challenged with vesicular stomatitis virus exhibit normal IgM and IgG production
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hematopoietic system
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• in response to anti-mu
• however, LPS activated B cell proliferation is normal
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• following stimulation with lection and TCR-CD3 cross-linking
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• the transition from double positive to single positive is blocked unlike in wild-type mice
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• the ratio of CD8+ to CD4+ T cells is skewed towards CD4+ T cells unlike in wild-type mice
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• in the spleen, lymph nodes, and bone marrow with CD8+ T cells reduced to a greater extent than CD4+ T cells
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• in mice exposed to lymphocytic choriomeningitis virus (LCMV)
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endocrine/exocrine glands
cellular
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• in response to anti-mu
• however, LPS activated B cell proliferation is normal
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• following stimulation with lection and TCR-CD3 cross-linking
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immune system
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• mice exhibit enhanced negative selection compared with wild-type mice
• positive selection of T cell is rescued and single positive thymic subsets compared to in Ptprctm1Mak homozygotes
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• SP4 thymocytes are reduced compared to in wild-type mice
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hematopoietic system
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• mice exhibit enhanced negative selection compared with wild-type mice
• positive selection of T cell is rescued and single positive thymic subsets compared to in Ptprctm1Mak homozygotes
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• SP4 thymocytes are reduced compared to in wild-type mice
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endocrine/exocrine glands
mortality/aging
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• mice die prematurely between 25 and 65 days of age
• Background Sensitivity: double mutants on mixed background (3 or less backcrosses to C57BL/6) show increased lifespan generally reaching 6 months of age and remaining healthy until 2 months of age, compared to accelerated phenotype of congenic mutants
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growth/size/body
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• double null mice are significantly underweight (60% of weight of wild-type mice)
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hematopoietic system
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• pre B cell numbers are similar in double null and wild-type mice, but few B cells develop further to CD19+ CD43- B cells
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• immature IgM+ IgD- B cells are decreased in number in the bone marrow
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• there is a higher proportion of transitional stage B cells, but absolute B cell number is decreased
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• spleens contain a cell population expressing CD19 but not IgM of IgD; cells are slightly bigger than normal B cells
• Background Sensitivity: myeloproliferative disorder with extramedullary hematopoiesis develops in congenic mice earlier than mice on mixed background
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• prior to death, mice develop a myeloproliferative disorder and show extramedullary hematopoiesis
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• pre-terminal animals show varying degrees of anemia
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• B cell lymphopenia develops; total cell numbers in spleens and lymph nodes are substatiantially lower than in wild-type mice or either single mutant homozygotes
• marked decreases in B cell numbers and percentages are observed in the bone marrow relative to controls
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• practically no recirculating mature IgM+ IgD+ B cells are detected in the bone marrow
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• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprjtm1.2Weis cells
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• mice develop myeloproliferative disorder and show distortion of splenic architecture
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immune system
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• pre B cell numbers are similar in double null and wild-type mice, but few B cells develop further to CD19+ CD43- B cells
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• immature IgM+ IgD- B cells are decreased in number in the bone marrow
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• there is a higher proportion of transitional stage B cells, but absolute B cell number is decreased
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• spleens contain a cell population expressing CD19 but not IgM of IgD; cells are slightly bigger than normal B cells
• Background Sensitivity: myeloproliferative disorder with extramedullary hematopoiesis develops in congenic mice earlier than mice on mixed background
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• B cell lymphopenia develops; total cell numbers in spleens and lymph nodes are substatiantially lower than in wild-type mice or either single mutant homozygotes
• marked decreases in B cell numbers and percentages are observed in the bone marrow relative to controls
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• practically no recirculating mature IgM+ IgD+ B cells are detected in the bone marrow
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• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprjtm1.2Weis cells
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• mice develop myeloproliferative disorder and show distortion of splenic architecture
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• extensive myeloid infiltration of liver is observed
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• scattered myeloid infiltrates are detected in the lungs
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liver/biliary system
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• extensive myeloid infiltration of liver is observed
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respiratory system
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• scattered myeloid infiltrates are detected in the lungs
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immune system
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• ligand-independent signaling checkpoint in T cell development is rescued compared to in Ptprcltng/Ptprctm1Mak heterozygotes and comparable to in Ptprcltng homozygotes
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hematopoietic system
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• ligand-independent signaling checkpoint in T cell development is rescued compared to in Ptprcltng/Ptprctm1Mak heterozygotes and comparable to in Ptprcltng homozygotes
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endocrine/exocrine glands
mortality/aging
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• Background Sensitivity: on mixed background, mice live until 6 months and remain healthy until 2 months of age, compared to mice on congenic background which display earlier lethality and accelerated B cell phenotype
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hematopoietic system
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• Fc receptor mediated phagocytosis by bone marrow-derived macrophages is decreased by 40% relative to wild-type
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• Background Sensitivity: on mixed background, myeloproliferative disorder in mice is delayed in development compared to congenic mutants
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• Background Sensitivity: young mice on mixed background show mild reduction in B cell number whereas reduction becomes more severe on congenic C57BL/6 background; B cell lymphopenia develops due to profound block at pre-B cell stage of development in the bone marrow
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• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprjtm1.2Weis cells
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• B cells show delayed calcium ion flux after B cell receptor crosslinking compared to wild-type and single null mice at 6-8 weeks of age
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immune system
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• Fc receptor mediated phagocytosis by bone marrow-derived macrophages is decreased by 40% relative to wild-type
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• Background Sensitivity: on mixed background, myeloproliferative disorder in mice is delayed in development compared to congenic mutants
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• Background Sensitivity: young mice on mixed background show mild reduction in B cell number whereas reduction becomes more severe on congenic C57BL/6 background; B cell lymphopenia develops due to profound block at pre-B cell stage of development in the bone marrow
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• in double mutants on mixed background, follicular lymph node B cells express higher amounts of IgM than wild-type or Ptprjtm1.2Weis cells
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• B cells show delayed calcium ion flux after B cell receptor crosslinking compared to wild-type and single null mice at 6-8 weeks of age
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• Fc receptor-induced tumor necrosis factor alpha production (Tnfa) by bone marrow-derived macrophages in double mutant mice
• after LPS treatment Tnfa production by macrophages is equivalent to wild-type cells
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cellular
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• Fc receptor mediated phagocytosis by bone marrow-derived macrophages is decreased by 40% relative to wild-type
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptprctm1Mak mutation
(2 available);
any
Ptprc mutation
(188 available)
Tg(TcraTcrb)425Cbn mutation
(3 available)
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immune system
hematopoietic system
immune system
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• fewer Tg(TcraTcrb)425Cbn CD4+ T cells develop compared to in wild-type Tg(TcraTcrb)425Cbn mice
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hematopoietic system
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• fewer Tg(TcraTcrb)425Cbn CD4+ T cells develop compared to in wild-type Tg(TcraTcrb)425Cbn mice
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endocrine/exocrine glands
Analysis Tools