Phenotypes associated with this allele

• Allele Symbol: Cebpd^tm1Pfj
• Allele Name: targeted mutation 1, Peter F Johnson
• Allele ID: MGI:2180841
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cebpd^tm1Pfj/Cebpd^tm1Pfj	involves: 129S1/Sv	MGI:5466357
hm2	Cebpd^tm1Pfj/Cebpd^tm1Pfj	involves: 129S1/Sv * C57BL/6	MGI:3606309
ht3	Cebpd^tm1Pfj/Cebpd^+	involves: 129S1/Sv	MGI:5466359

Genotype
MGI:5466357

hm1

• Allelic Composition: Cebpd^tm1Pfj/Cebpd^tm1Pfj
• Genetic Background: involves: 129S1/Sv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased blood uric acid level ( J:116932 )

• decrease in serum levels of urate

decreased circulating lactate level ( J:116932 )

• 90% decrease in serum levels of lactate

abnormal urine homeostasis ( J:116932 )

• 29-fold increase in urinary lactate levels

increased urine uric acid level ( J:116932 )

• 6-fold increase in urinary excretion of urate

renal/urinary system

abnormal urine homeostasis ( J:116932 )

• 29-fold increase in urinary lactate levels

increased urine uric acid level ( J:116932 )

• 6-fold increase in urinary excretion of urate

cellular

increased mammary gland epithelial cell proliferation ( J:103094 )

♀ • the BrdU labeling index is higher in mammary epithelial cells of nulliparous females compared to controls during the proestrus/estrus and metestrus/diestrus phases of the estrus cycle, indicating increased proliferation of these cells

♀ • however, apoptosis levels are similar to controls

endocrine/exocrine glands

increased mammary gland epithelial cell proliferation ( J:103094 )

♀ • the BrdU labeling index is higher in mammary epithelial cells of nulliparous females compared to controls during the proestrus/estrus and metestrus/diestrus phases of the estrus cycle, indicating increased proliferation of these cells

♀ • however, apoptosis levels are similar to controls

abnormal mammary gland epithelium morphology ( J:103094 )

♀ • mean total mammary gland cellular volume occupied by epithelium is higher in adult nulliparous females than in controls

abnormal branching of the mammary ductal tree ( J:103094 )

♀ • with increasing age (by 26 weeks of age), adult nulliparous females exhibit increased ductal arborization showing a higher mean number of mammary ductal branches than wild-type females

♀ • however, mammary gland involution appears normal and no differences in mammary gland morphology, ductal branching or total epithelial content is seen in multiparous females

integument

increased mammary gland epithelial cell proliferation ( J:103094 )

♀ • the BrdU labeling index is higher in mammary epithelial cells of nulliparous females compared to controls during the proestrus/estrus and metestrus/diestrus phases of the estrus cycle, indicating increased proliferation of these cells

♀ • however, apoptosis levels are similar to controls

abnormal mammary gland epithelium morphology ( J:103094 )

♀ • mean total mammary gland cellular volume occupied by epithelium is higher in adult nulliparous females than in controls

abnormal branching of the mammary ductal tree ( J:103094 )

♀ • with increasing age (by 26 weeks of age), adult nulliparous females exhibit increased ductal arborization showing a higher mean number of mammary ductal branches than wild-type females

♀ • however, mammary gland involution appears normal and no differences in mammary gland morphology, ductal branching or total epithelial content is seen in multiparous females

Genotype
MGI:3606309

hm2

• Allelic Composition: Cebpd^tm1Pfj/Cebpd^tm1Pfj
• Genetic Background: involves: 129S1/Sv * C57BL/6

mortality/aging

mortality/aging ( J:49799 )

N • homozygotes are viable, fertile and developmentally normal; no significant differences in body weight, core body temperature, or hematology are observed

behavior/neurological

abnormal contextual conditioning behavior ( J:49799 )

• at 3 months, homoygotes exhibit normal brain neurochemistry with respect to total monoamine content, as well as normal spatial learning (Morris water maze task), locomotor activity (open-field), motor function (rotarod), and anxiety-like behavior (light/dark exploration test)

• however, homozygotes exhibit a selective increase in Pavlovian conditioned fear, with markedly increased freezing levels after long (i.e 24-hr) retention intervals; no significant differences are noted during the 30-min context test

• notably, homozygotes do not exhibit enhanced auditory-cue-conditioned fear, and serotonin and norepinephrine levels remain normal in mutant cortex

homeostasis/metabolism

abnormal cytokine level ( J:192313 )

• TNF-alpha, IL-6, and MIP-2 levels are decreased by 85%, 60% and 45%, respectively, in mutants compared to wild-type mice following intratracheal instillation of LPS

decreased susceptibility to injury ( J:192313 )

• mutants exhibit reduced lung injury following intratracheal instillation of lipopolysaccharide (LPS), showing decreased neutrophil accumulation, negligible intra-alveolar hemorrhage, diminished albumin levels in bronchial alveolar lavage (BAL) fluids, decrease in MPO activity, fewer neutrophils but increased macrophages in BAL fluids, and decreased TNF-alpha, IL-6 and MIP-2 levels compared to similarly treated wild-type mice

immune system

abnormal cytokine level ( J:192313 )

• TNF-alpha, IL-6, and MIP-2 levels are decreased by 85%, 60% and 45%, respectively, in mutants compared to wild-type mice following intratracheal instillation of LPS

decreased inflammatory response ( J:192313 )

• mutants exhibit reduced inflammatory cell influx into the lungs following intratracheal instillation of LPS to induce lung damage compared to wild-type mice, showing attenuation of neutrophil accumulation

respiratory system

abnormal respiratory system physiology ( J:192313 )

• mutants exhibit reduced lung injury following intratracheal instillation of LPS, showing decreased neutrophil accumulation, negligible intra-alveolar hemorrhage, diminished albumin levels in BAL fluids, decrease in MPO activity, fewer neutrophils but increased macrophages in BAL fluids, and decreased TNF-alpha, IL-6 and MIP-2 levels compared to similarly treated wild-type mice

Genotype
MGI:5466359

ht3

• Allelic Composition: Cebpd^tm1Pfj/Cebpd⁺
• Genetic Background: involves: 129S1/Sv

homeostasis/metabolism

decreased circulating lactate level ( J:116932 )

• 78% decrease in serum levels of lactate

abnormal urine homeostasis ( J:116932 )

• 4-fold increase in urinary lactate levels

increased urine uric acid level ( J:116932 )

• 2.5-fold increase in urinary excretion of urate

renal/urinary system

abnormal urine homeostasis ( J:116932 )

• 4-fold increase in urinary lactate levels

increased urine uric acid level ( J:116932 )

• 2.5-fold increase in urinary excretion of urate