Phenotypes associated with this allele

• Allele Symbol: Cul1^tmTye
• Allele Name: targeted mutation 1, Michael Tyers
• Allele ID: MGI:2180659
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cul1^tmTye/Cul1^tmTye	involves: 129S1/Sv * 129X1/SvJ	MGI:3044913

Genotype
MGI:3044913

hm1

• Allelic Composition: Cul1^tmTye/Cul1^tmTye
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:58150 )

• mutant embryos failed to survive beyond E7.5

embryo

abnormal ectoderm development ( J:58150 )

• the embryonic ectoderm appeared thin and contained many condensed nuclei

embryonic growth arrest ( J:58150 )

• mutant embryos arrested prior to the onset of gastrulation at approximately E6.5

decreased embryo size ( J:58150 )

• mutant embryos stopped developing at E6.5, and were significantly smaller than wild-type by E7.5

growth/size/body

decreased embryo size ( J:58150 )

• mutant embryos stopped developing at E6.5, and were significantly smaller than wild-type by E7.5

cellular

abnormal cell physiology ( J:58150 )

• at E6.5, mutant embryos showed a global elevation in cyclin E1 protein levels, in the absence of altered levels of cyclin E1 mRNA

• outgrowths of mutant blastocysts failed to proliferate past 7 days in culture; trophoblast giant cells continued to endocycle despite elevated cyclin E1 levels

increased apoptosis ( J:58150 )

• at E6.5, the developmental arrest was associated with a modest increase in apoptotic cells