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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ptger4tm1Aic
targeted mutation 1, Atsushi Ichikawa
MGI:2137851
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Ptger4tm1Aic/Ptger4tm1Aic involves: 129P2/OlaHsd * C57BL/6 MGI:2175006
hm2
Ptger4tm1Aic/Ptger4tm1Aic involves: 129P2/OlaHsd * C57BL/6Cr MGI:3590537


Genotype
MGI:2175006
hm1
Allelic
Composition
Ptger4tm1Aic/Ptger4tm1Aic
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptger4tm1Aic mutation (0 available); any Ptger4 mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• less than 5% of homozygotes survive and grow normally for more than 1 year (J:47618)
• less than 5% of homozygotes survive and grow normally for more than 1 year (J:47618)
• greater than 95% of F2 homozygotes die within 72 hours after birth (J:47618)
• greater than 95% of F2 homozygotes die within 72 hours after birth (J:47618)

cardiovascular system
N
• F2 homozygotes obtained by selective bleeding exhibit normal basal heart rate levels relative to wild-type mice (J:92865)
• in a two-kidney, one-clip hypertension model (2K1C), F2 homozygotes obtained by selective bleeding show no significant differences in the degree and time-course of renovascular hypertension relative to F2 wild-type mice (J:92865)
• no significant changes in heart rate levels or plasma creatinine levels are noted at day 14 of 2K1C (J:92865)
• F2 homozygotes obtained by selective bleeding exhibit normal basal heart rate levels relative to wild-type mice (J:92865)
• in a two-kidney, one-clip hypertension model (2K1C), F2 homozygotes obtained by selective bleeding show no significant differences in the degree and time-course of renovascular hypertension relative to F2 wild-type mice (J:92865)
• no significant changes in heart rate levels or plasma creatinine levels are noted at day 14 of 2K1C (J:92865)
• moribund homozygotes display dilated pulmonary arteries (J:47618)
• moribund homozygotes display dilated pulmonary arteries (J:47618)
• homozygotes display significant congestion of pulmonary capillaries (J:47618)
• homozygotes display significant congestion of pulmonary capillaries (J:47618)
• the DA remains fully open with a thin wall in 6-hr-old homozygotes that still appear healthy, but fails to close completely in moribund neonates (J:47618)
• surviving homozygotes exhibit a DA that is either partially closed or severely narrowed (J:47618)
• the DA remains fully open with a thin wall in 6-hr-old homozygotes that still appear healthy, but fails to close completely in moribund neonates (J:47618)
• surviving homozygotes exhibit a DA that is either partially closed or severely narrowed (J:47618)
• moribund homozygotes exhibit left-sided heart failure (J:47618)
• moribund homozygotes exhibit left-sided heart failure (J:47618)

respiratory system
• homozygotes display significant congestion of pulmonary capillaries (J:47618)
• homozygotes display significant congestion of pulmonary capillaries (J:47618)
• moribund homozygotes exhibit disorganized and shrunken alveoli (J:47618)
• moribund homozygotes exhibit disorganized and shrunken alveoli (J:47618)

behavior/neurological
• homozygotes become lethargic with significantly weakened movements at ~24 hours after birth (J:47618)
• homozygotes become lethargic with significantly weakened movements at ~24 hours after birth (J:47618)

homeostasis/metabolism
N
• homozygotes exhibit a normal febrile response to PGE2 (J:70534)
• homozygotes exhibit a normal febrile response to PGE2 (J:70534)
• on a low-salt diet, F2 homozygotes obtained by selective bleeding display an increase in plasma renin activity that is comparable to that in F2 wild-type mice (J:92865)
• on a low-salt diet, F2 homozygotes obtained by selective bleeding display an increase in plasma renin activity that is comparable to that in F2 wild-type mice (J:92865)

skeleton
• in culture, TRAP-positive osteoclasts are formed in dibutyryl cAMP-treated calvaria, but not in PGE2-treated calvaria (J:63070)
• PGE2-induced osteoclast formation is also diminished in bone marrow cultures (not shown) (J:63070)
• in culture, TRAP-positive osteoclasts are formed in dibutyryl cAMP-treated calvaria, but not in PGE2-treated calvaria (J:63070)
• PGE2-induced osteoclast formation is also diminished in bone marrow cultures (not shown) (J:63070)
• in culture, calvaria and long bones isolated from mutant neonates show a significant reduction in PGE2-induced bone resorption (J:63070)
• in contrast, dibutyryl cAMP-stimulated bone resorption occurs similarly in wild-type and mutant calvaria and long bones (J:63070)
• notably, PGE2-induction of MMP-2 and MMP-13 is markedly impaired in calvarial cultures from mutant neonates (J:63070)
• in culture, calvaria and long bones isolated from mutant neonates show a significant reduction in PGE2-induced bone resorption (J:63070)
• in contrast, dibutyryl cAMP-stimulated bone resorption occurs similarly in wild-type and mutant calvaria and long bones (J:63070)
• notably, PGE2-induction of MMP-2 and MMP-13 is markedly impaired in calvarial cultures from mutant neonates (J:63070)

immune system
N
• ovalbumin-sensitized homozygotes display a normal delayed-type hypersensitivity response, as measured by footpad swelling after ovalbumin challenge (J:83725)
• in addition, homozygotes exhibit a normal acute inflammatory response to 12-O-tetradecanoylphorbol-13-acetate (J:83725)
• ovalbumin-sensitized homozygotes display a normal delayed-type hypersensitivity response, as measured by footpad swelling after ovalbumin challenge (J:83725)
• in addition, homozygotes exhibit a normal acute inflammatory response to 12-O-tetradecanoylphorbol-13-acetate (J:83725)
• in a model of IgE antigen-dependent passive cutaneous anaphylaxis, mice exhibit normal dye extravasation (a measure of edema) (J:197334)
• in a model of IgE antigen-dependent passive cutaneous anaphylaxis, mice exhibit normal dye extravasation (a measure of edema) (J:197334)
• in culture, TRAP-positive osteoclasts are formed in dibutyryl cAMP-treated calvaria, but not in PGE2-treated calvaria (J:63070)
• PGE2-induced osteoclast formation is also diminished in bone marrow cultures (not shown) (J:63070)
• in culture, TRAP-positive osteoclasts are formed in dibutyryl cAMP-treated calvaria, but not in PGE2-treated calvaria (J:63070)
• PGE2-induced osteoclast formation is also diminished in bone marrow cultures (not shown) (J:63070)
• DNFB-sensitized homozygotes display reduced antigen-specific T-cell proliferation in the regional lymph nodes (J:83725)
• DNFB-sensitized homozygotes display reduced antigen-specific T-cell proliferation in the regional lymph nodes (J:83725)
• in culture, calvaria and long bones isolated from mutant neonates show a significant reduction in PGE2-induced bone resorption (J:63070)
• in contrast, dibutyryl cAMP-stimulated bone resorption occurs similarly in wild-type and mutant calvaria and long bones (J:63070)
• notably, PGE2-induction of MMP-2 and MMP-13 is markedly impaired in calvarial cultures from mutant neonates (J:63070)
• in culture, calvaria and long bones isolated from mutant neonates show a significant reduction in PGE2-induced bone resorption (J:63070)
• in contrast, dibutyryl cAMP-stimulated bone resorption occurs similarly in wild-type and mutant calvaria and long bones (J:63070)
• notably, PGE2-induction of MMP-2 and MMP-13 is markedly impaired in calvarial cultures from mutant neonates (J:63070)
• at 24 hrs after FITC exposure, female homozygotes of the F2 progeny show a significantly reduced accumulation of Langerhans cells in their regional lymph nodes (J:83725)
in vitro, mutant skin explants exhibit reduced Langerhans cell emigration into the medium relative to wild-type explants; emigration is not affected by an EP4 agonist or antagonist (J:83725)
• at 24 hrs after FITC exposure, female homozygotes of the F2 progeny show a significantly reduced accumulation of Langerhans cells in their regional lymph nodes (J:83725)
in vitro, mutant skin explants exhibit reduced Langerhans cell emigration into the medium relative to wild-type explants; emigration is not affected by an EP4 agonist or antagonist (J:83725)
• homozygotes exhibit reduced ear swelling, spongiosis and infiltration of lymphocytes in the edematous dermis in the contact hypersensitivity reaction, using dinitrofluorobenzene as the sensitizing agent (J:83725)
• homozygotes exhibit reduced ear swelling, spongiosis and infiltration of lymphocytes in the edematous dermis in the contact hypersensitivity reaction, using dinitrofluorobenzene as the sensitizing agent (J:83725)
• lymphocytes isolated from regional lymph nodes of DNBS-sensitized homozygotes show a significant reduction in IFN-gamma production (J:83725)
• lymphocytes isolated from regional lymph nodes of DNBS-sensitized homozygotes show a significant reduction in IFN-gamma production (J:83725)

hematopoietic system
• in culture, TRAP-positive osteoclasts are formed in dibutyryl cAMP-treated calvaria, but not in PGE2-treated calvaria (J:63070)
• PGE2-induced osteoclast formation is also diminished in bone marrow cultures (not shown) (J:63070)
• in culture, TRAP-positive osteoclasts are formed in dibutyryl cAMP-treated calvaria, but not in PGE2-treated calvaria (J:63070)
• PGE2-induced osteoclast formation is also diminished in bone marrow cultures (not shown) (J:63070)
• DNFB-sensitized homozygotes display reduced antigen-specific T-cell proliferation in the regional lymph nodes (J:83725)
• DNFB-sensitized homozygotes display reduced antigen-specific T-cell proliferation in the regional lymph nodes (J:83725)
• in culture, calvaria and long bones isolated from mutant neonates show a significant reduction in PGE2-induced bone resorption (J:63070)
• in contrast, dibutyryl cAMP-stimulated bone resorption occurs similarly in wild-type and mutant calvaria and long bones (J:63070)
• notably, PGE2-induction of MMP-2 and MMP-13 is markedly impaired in calvarial cultures from mutant neonates (J:63070)
• in culture, calvaria and long bones isolated from mutant neonates show a significant reduction in PGE2-induced bone resorption (J:63070)
• in contrast, dibutyryl cAMP-stimulated bone resorption occurs similarly in wild-type and mutant calvaria and long bones (J:63070)
• notably, PGE2-induction of MMP-2 and MMP-13 is markedly impaired in calvarial cultures from mutant neonates (J:63070)

integument
• homozygotes become pale at ~24 hours after birth (J:47618)
• homozygotes become pale at ~24 hours after birth (J:47618)

cellular
• the DA remains fully open with a thin wall in 6-hr-old homozygotes that still appear healthy, but fails to close completely in moribund neonates (J:47618)
• surviving homozygotes exhibit a DA that is either partially closed or severely narrowed (J:47618)
• the DA remains fully open with a thin wall in 6-hr-old homozygotes that still appear healthy, but fails to close completely in moribund neonates (J:47618)
• surviving homozygotes exhibit a DA that is either partially closed or severely narrowed (J:47618)
• in culture, TRAP-positive osteoclasts are formed in dibutyryl cAMP-treated calvaria, but not in PGE2-treated calvaria (J:63070)
• PGE2-induced osteoclast formation is also diminished in bone marrow cultures (not shown) (J:63070)
• in culture, TRAP-positive osteoclasts are formed in dibutyryl cAMP-treated calvaria, but not in PGE2-treated calvaria (J:63070)
• PGE2-induced osteoclast formation is also diminished in bone marrow cultures (not shown) (J:63070)

Mouse Models of Human Disease
OMIM ID Ref(s)
Patent Ductus Arteriosus 607411 J:47618




Genotype
MGI:3590537
hm2
Allelic
Composition
Ptger4tm1Aic/Ptger4tm1Aic
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6Cr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptger4tm1Aic mutation (0 available); any Ptger4 mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
tumorigenesis
• male suvivors of the F2 progeny treated with colon carcinogen azoxymethane exhibit significantly reduced numbers of aberrant crypt foci per colon (56% of wild-type values) (J:73944)
• male suvivors of the F2 progeny treated with colon carcinogen azoxymethane exhibit significantly reduced numbers of aberrant crypt foci per colon (56% of wild-type values) (J:73944)
• all male homozygotes treated with colon carcinogen azoxymethane develop aberrant crypt foci, i.e. putative preneoplastic lesions in the colon, albeit with reduced numbers of foci per colon (J:73944)
• preneoplastic lesions are not associated with significant changes in body or organ weights (J:73944)
• all male homozygotes treated with colon carcinogen azoxymethane develop aberrant crypt foci, i.e. putative preneoplastic lesions in the colon, albeit with reduced numbers of foci per colon (J:73944)
• preneoplastic lesions are not associated with significant changes in body or organ weights (J:73944)

homeostasis/metabolism
• male suvivors of the F2 progeny treated with colon carcinogen azoxymethane exhibit significantly reduced numbers of aberrant crypt foci per colon (56% of wild-type values) (J:73944)
• male suvivors of the F2 progeny treated with colon carcinogen azoxymethane exhibit significantly reduced numbers of aberrant crypt foci per colon (56% of wild-type values) (J:73944)





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last database update
02/02/2016
MGI 6.02
The Jackson Laboratory