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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ighmtm1Che
targeted mutation 1, Jianzhu Chen
MGI:1934014
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Ighmtm1Che/Ighmtm1Che involves: 129 * C57BL/6 MGI:3834169
hm2
Ighmtm1Che/Ighmtm1Che involves: 129S4/SvJae * C57BL/6 MGI:2179104


Genotype
MGI:3834169
hm1
Allelic
Composition
Ighmtm1Che/Ighmtm1Che
Genetic
Background
involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ighmtm1Che mutation (1 available); any Ighm mutation (34 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• mice are able to clear a Giardia muris infection in a manner similar to wild-type mice




Genotype
MGI:2179104
hm2
Allelic
Composition
Ighmtm1Che/Ighmtm1Che
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ighmtm1Che mutation (1 available); any Ighm mutation (34 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• in spleen of mutants, a 2.6-fold increase in B-1 cells is detected
• in peritoneum, B-1 B cell numbers are elevated ~3-fold at 4 weeks of age through 12 months
• after LPS challenge, marginal zone metallophilic -2+ (MOMA-2+) macrophages of the T and B cell zone fail to establish a ring of complement 3 (C3)-producing macrophages in the outer B cell zone close to the marginal zone
• 72 hours after LPS challenge, spleen white pulp area continues to deteriorate; B cell follicles dissolve
• 7 days after secondary immunization with suboptimal dose of T cell-dependent antigen NP-KLH, number and size of germinal centers in mutant spleens are reduced compared to controls; number is reduced ~4fold, but numbers are normal in mice immunized with 10 or 100 ug of antigen
• germinal center reactions are also impaired when 1 ug of NP-KLH antigen is used for immunization
• after 72 hours of LPS treatment, marginal zone is no longer structured; rings of 2F8+, ER-TR9+, and MOMA-1+ macrophages are not detectable
• T cell zone shows severe destruction with disorganized B and T cells 72 hours after LPS treatment
• germinal center reactions are impaired when 1 ug of NP-KLH antigen is used for immunization
• antibody affinity maturation is compromised in response to suboptimal doses of antigen (NP5-BSA or NP15-BSA)
• elevated in serum of mutants at young ages
• IgG1 levels after immunization with a suboptimal dose (<10 ug) of T cell-dependent antigen NP-KLH >100-fold lower in mutants
• elevated in serum of mutants at 4 weeks, but difference disappears as mice mature
• treatment with the T cell independent antigen NP-Ficoll results in 3-4-fold increase in IgG2a at 7 and 14 days after immunization
• treatment with the T cell independent antigen NP-Ficoll reduced IgG2b levels after immunization
• elevated in serum of mutants at 4 weeks, but difference disappears as mice mature
• IgM levels in serum are ~0.1 ug/ml compared to ~250 ug/ml in serum of wild-type controls; levels do not increase in mice up to 1 year in age
• antigen trapping on follicular dendritic cells (FDCs) in spleen germinal centers is impaired when 10 ug of NP-KLH antigen is used for immunization; no antigen trapping is detected on FDCs 12 days after immunization, even though titers of NP-specific IgG antibodies are comparable to wild-type
• treatment with the T cell independent antigen NP-Ficoll results in 3-4-fold increase in IgG2a at 7 and 14 days after immunization
• IgG responses to T cell-dependent antigen NP-KLH are impaired at suboptimal antigen doses; at a suboptimal dose 7 days after immunization, IgG antibody levels are ~25-fold lower for primary and secondary responses relative to heterozygous controls

hematopoietic system
• in spleen of mutants, a 2.6-fold increase in B-1 cells is detected
• in peritoneum, B-1 B cell numbers are elevated ~3-fold at 4 weeks of age through 12 months
• after LPS challenge, marginal zone metallophilic -2+ (MOMA-2+) macrophages of the T and B cell zone fail to establish a ring of complement 3 (C3)-producing macrophages in the outer B cell zone close to the marginal zone
• 72 hours after LPS challenge, spleen white pulp area continues to deteriorate; B cell follicles dissolve
• 7 days after secondary immunization with suboptimal dose of T cell-dependent antigen NP-KLH, number and size of germinal centers in mutant spleens are reduced compared to controls; number is reduced ~4fold, but numbers are normal in mice immunized with 10 or 100 ug of antigen
• germinal center reactions are also impaired when 1 ug of NP-KLH antigen is used for immunization
• after 72 hours of LPS treatment, marginal zone is no longer structured; rings of 2F8+, ER-TR9+, and MOMA-1+ macrophages are not detectable
• T cell zone shows severe destruction with disorganized B and T cells 72 hours after LPS treatment
• elevated in serum of mutants at young ages
• IgG1 levels after immunization with a suboptimal dose (<10 ug) of T cell-dependent antigen NP-KLH >100-fold lower in mutants
• elevated in serum of mutants at 4 weeks, but difference disappears as mice mature
• treatment with the T cell independent antigen NP-Ficoll results in 3-4-fold increase in IgG2a at 7 and 14 days after immunization
• treatment with the T cell independent antigen NP-Ficoll reduced IgG2b levels after immunization
• elevated in serum of mutants at 4 weeks, but difference disappears as mice mature
• IgM levels in serum are ~0.1 ug/ml compared to ~250 ug/ml in serum of wild-type controls; levels do not increase in mice up to 1 year in age





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory