Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myf6tm1Eno mutation
(0 available);
any
Myf6 mutation
(19 available)
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skeleton
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• rib anomalies are bilaterally asymmetric and appear to occur randomly in different ribs but show 100% penetrance
• newborns show supernumerary processes in the distal regions of the ribs
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• many ribs appear to emerge from the vertebral bodies at incorrect angles
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• multiple bifurcations
(J:26105)
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• distal parts of ribs are missing resulting in short ribs
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muscle
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N |
• homozygotes exhibit normal distribution of fast- and slow-twitch muscle fibers and normal somite myogenesis
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• mice have mild extraocular muscle defects compared to Myf5tm2Tajb homozygotes
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• myotomes consist of very few myocytes at E10.5 and the myocytes are poorly organized relative to each other, resulting in misshapen and small myotomes
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vision/eye
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• mice have mild extraocular muscle defects compared to Myf5tm2Tajb homozygotes
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Allelic
Composition |
Myf5tm1Jae/Myf5+
Myf6tm1Eno/Myf6+
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Genetic
Background |
involves: 129S4/SvJae * 129S7/SvEvBrd |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myf5tm1Jae mutation
(1 available);
any
Myf5 mutation
(17 available)
Myf6tm1Eno mutation
(0 available);
any
Myf6 mutation
(19 available)
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mortality/aging
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• 63% of double heterozygous mice, in which the Myf5 and Myf6 alleles are on different chromosomes and show severely reduced expression of Myf5, die immediately after birth; the rest survive
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skeleton
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• attachment failure of at least one rib to the sternum is seen in most mutants, although the identity of the affected rib(s) and the number of ribs affected varies
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muscle
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• number of myocytes in the myotomes is reduced at E10.5 and the conformation of the myotome is abnormal
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myf6tm1Eno mutation
(0 available);
any
Myf6 mutation
(19 available)
Myod1tm1Jae mutation
(2 available);
any
Myod1 mutation
(23 available)
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mortality/aging
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• die within minutes after birth due to an inability to breathe
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growth/size/body
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• decrease in body mass at E16.5
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muscle
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• nuclei are centrally located instead of in the periphery as in wild-type
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• severe skeletal muscle deficiency with only residual muscle fibers surrounded by mononucleated cells
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• appear to lack skeletal muscle beginning at E14.5
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skeleton
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• show rib defects indistinguishable from Myf6tm1Eno homozygotes
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• show abnormal curvature of the spine starting around E14.5
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adipose tissue
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• accumulate brown fat at the apex of the neck beginning at E14.5
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respiratory system
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• die within minutes after birth due to an inability to breathe
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myf6tm1Eno mutation
(0 available);
any
Myf6 mutation
(19 available)
Myod1tm1Jae mutation
(2 available);
any
Myod1 mutation
(23 available)
Myogtm1Whk mutation
(0 available);
any
Myog mutation
(14 available)
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normal phenotype
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• mutants are normal, viable and fertile and appear to have normal muscle
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myf6tm1Eno mutation
(0 available);
any
Myf6 mutation
(19 available)
Myod1tm1Jae mutation
(2 available);
any
Myod1 mutation
(23 available)
Myogtm1Whk mutation
(0 available);
any
Myog mutation
(14 available)
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muscle
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• myogenesis is arrested at the level seen in single homozygous Myog mutants
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myf6tm1Eno mutation
(0 available);
any
Myf6 mutation
(19 available)
Myod1tm1Jae mutation
(2 available);
any
Myod1 mutation
(23 available)
Myogtm1Whk mutation
(0 available);
any
Myog mutation
(14 available)
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mortality/aging
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• die within minutes of birth
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growth/size/body
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• E18.5 mutants show a reduction in body mass
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muscle
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• marker analysis indicates that myoblasts are present in normal muscles but they are unable to differentiate into muscle fibers
marker analysis indicates that myoblasts are present in normal muscles but they are unable to differentiate into muscle fibers
• myoblasts from neonates are unable to differentiate in vitro
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• severe muscle deficiency with almost no muscle fibers; the few fibers that are present are extremely thin and underdeveloped
• tongue, back, limb, and skeletal muscle are all affected comparably unlike in single Myog homozygous mutants
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skeleton
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• the average lengths of the ossified portions of the ribs are 30% shorter than normal at E15.5, however the ribs reach the sternum
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myf6tm1Eno mutation
(0 available);
any
Myf6 mutation
(19 available)
Myogtm1Whk mutation
(0 available);
any
Myog mutation
(14 available)
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mortality/aging
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• lethality at birth; mutants exhibit reduced levels of Myf5 mRNA unlike single Myf6tm1Eno homozygotes
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muscle
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• exhibit a deficiency in muscle fibers showing only residual differentiated muscle fibers, however the number of these residual fibers is similar to that seen in Myog homozygotes
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• exhibit a deficiency in muscle fibers
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skeleton
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• sternebral bodies are severely malformed
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• distal portions of the ribs fail to reach the sternum
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• upper region of the sternum is bifurcated
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Analysis Tools