Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dstncorn1 mutation
(0 available);
any
Dstn mutation
(43 available)
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The Dstncorn1/Dstncorn1 mouse
vision/eye
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• neutrophil and macrophage infiltration of the cornea
• infiltration by neutrophils is seen by P12 and persists through P28
• neutrophils and macrophages are most abundant in the periphery at P14
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immune system
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N |
• no significant difference is detected in the peripheral blood populations of neutrophils or macrophages compared to controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dstncorn1 mutation
(0 available);
any
Dstn mutation
(43 available)
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vision/eye
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• neovascularization is present in limbus at E18; vascularization grows and reaches center of cornea at 45 days
(J:31798)
• hemangiogenesis in the normally avascular cornea is first seen as single sprouts around 2 weeks of age, by 3 weeks of age slit-lamp examination shows outgrowth of limbal blood vessels, and blood vessels cover the corneal surface by approximately 6 weeks of age.
(J:98465)
• corneal hemangiogenesis precedes lymphangiogenesis by approximately one week, and there is no corneal blood vessel regression in the first year
(J:98465)
• corneal hemangiogenesis and lymphangiogenesis can be inhibited by treatment with an antibody blocking vascular enothelial growht factor receptor 3
(J:98465)
• mice exhibit spontaneous corneal vascularization
(J:114973)
• corneal injection of sflt-1 (secreted isoform of the cell surface receptor membrane-bound Flt1) protein reduces the area of vascularization compared to untreated mutants
(J:114973)
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• focal areas ~1 mm in diameter are elevated above corneal surface
(J:31798)
• epithelium has "dry", white appearance
(J:31798)
• at 12 days of age, mice show mild edema of basal epithelial layers and corneal surface shows irregularities with focal areas ~1 mm in diameter elevated above corneal surface; corneal surface is increasingly roughened and irregular, until 70 days of age when irregularity stabilizes
(J:31798)
• surface epithelial cell nuclei are pyknotic and cytoplasm has granular appearance
(J:31798)
• eosinophilia is seen in superficial epithelial cells at 12 days of age, but is decreased by 60 days of age
(J:31798)
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• at 21 and 30 days of age, thickening of epithelium increases
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• at 4 weeks of age the hyperplastic corneal epithelium has altered expression of keratin 14, involucrin, and lumican
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• bilateral cataracts develop by 45-50 days of age; progressive lenticular opacification is observed to 8 months of age
• cataracts are manifested by liquefaction of lens fibers and posterior migration of lens epithelial nuclei
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cellular
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• in homozygotes, there are 10-fold more proliferating corneal epithelial cells than in wild-type or heterozygous mice
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cardiovascular system
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• neovascularization is present in limbus at E18; vascularization grows and reaches center of cornea at 45 days
(J:31798)
• hemangiogenesis in the normally avascular cornea is first seen as single sprouts around 2 weeks of age, by 3 weeks of age slit-lamp examination shows outgrowth of limbal blood vessels, and blood vessels cover the corneal surface by approximately 6 weeks of age.
(J:98465)
• corneal hemangiogenesis precedes lymphangiogenesis by approximately one week, and there is no corneal blood vessel regression in the first year
(J:98465)
• corneal hemangiogenesis and lymphangiogenesis can be inhibited by treatment with an antibody blocking vascular enothelial growht factor receptor 3
(J:98465)
• mice exhibit spontaneous corneal vascularization
(J:114973)
• corneal injection of sflt-1 (secreted isoform of the cell surface receptor membrane-bound Flt1) protein reduces the area of vascularization compared to untreated mutants
(J:114973)
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immune system
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N |
• the aqueous humor has a normal protein and leukocyte count indicating that the corneal neovascularization and lymphangiogenesis are not mediated primarily by inflammation
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• Corneal lymphangiogenesis begins with small sprouts around 3 weeks of age, a week after corneal hemangiogenesis begins, and lymphatic vessels are clearly visible by 4 to 6 weeks of age. Vessel fragmentation is seen as early as 2 months of age. Thus, corneal lymphangiogenesis is less severe than hemangiogenesis and comparatively delayed.
• This corneal lymphangiogenesis occurs in a low inflammatory context in which there are only slightly more CD45 positive cells at 4 weeks of age than in controls.
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Allelic
Composition |
Dstncorn1/Dstncorn1
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Genetic
Background |
involves: A/WySn * brachy stock * C57BL/6JSmn |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dstncorn1 mutation
(0 available);
any
Dstn mutation
(43 available)
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vision/eye
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• severe epithelial proliferation
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cardiovascular system
Allelic
Composition |
Dstncorn1/Dstncorn1-2J
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Genetic
Background |
involves: A/WySn * brachy stock * C57BL/6JSmn |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dstncorn1 mutation
(0 available);
any
Dstn mutation
(43 available)
Dstncorn1-2J mutation
(1 available);
any
Dstn mutation
(43 available)
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vision/eye
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N |
• unlike Dstncorn1 homozygotes corneal vascularization is not seen
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• epithelial proliferation
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cxcr2tm1Mwm mutation
(3 available);
any
Cxcr2 mutation
(31 available)
Dstncorn1 mutation
(0 available);
any
Dstn mutation
(43 available)
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vision/eye
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• compared to Dstncorn1 homozygotes with at least one wild type allele of Il8rb, neutrophil recruitment to the cornea is almost completely inhibited; however, macrophages recruitment is still seen
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• the decrease in neutrophil recruitment did not affect corneal vascularization
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cardiovascular system
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• the decrease in neutrophil recruitment did not affect corneal vascularization
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cfl1c5 mutation
(0 available);
any
Cfl1 mutation
(25 available)
Dstncorn1 mutation
(0 available);
any
Dstn mutation
(43 available)
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mortality/aging
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• unable to recover embryos at post-implantation stages
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cfl1c5 mutation
(0 available);
any
Cfl1 mutation
(25 available)
Dstncorn1 mutation
(0 available);
any
Dstn mutation
(43 available)
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mortality/aging
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• most arrest prior to node formation
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embryo
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• most arrest prior to node formation
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• in the 3 embryos that develop nodes apical trafficking of planar cell polarity proteins is disrupted
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Analysis Tools