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Allele Symbol Allele Name Allele ID |
Cd14tm1Smg targeted mutation 1, Sanna M Goyert MGI:1857977 |
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| Summary |
5 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• eight hours after infection with E. coli, mice have 10-fold lower bacterial counts in the blood as do infection wild-type controls
• the bacterial resistance is abolished when mice are made neutropenic by cyclophosphamide treatment
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• increased numbers of neutrophils migrate to the peritoneal cavity after i.p. infection with E. coli or after injection with LPS
• mice have about 5-fold more neutrophils in the peritoneal cavity than controls during the first 5 hour post-injection
• detectable amounts of neutrophils are present within 3 hours of LPS injection while neutrophils are not observed in wild-type controls until 24 hours after LPS injection
• neutrophil response can be induced with very low amounts of LPS
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• macrophages are less responsive to LPS compared to controls as determined by TNF secretion
• macrophages are least responsive to carbohydrated (i.e. smooth) forms of LPS where 150,000 fold more is needed to generate the same response as in controls
• in contrast, macrophages are only 4-fold less responsive to the non-carbohydrated form of LPS known as lipid A
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• macrophages produce less TNF in response LPS than in controls
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• eight hours after infection with E. coli, mice have 10-fold lower bacterial counts in the blood as do infection wild-type controls
• the bacterial resistance is abolished when mice are made neutropenic by cyclophosphamide treatment
|
|
• increased numbers of neutrophils migrate to the peritoneal cavity after i.p. infection with E. coli or after injection with LPS
• mice have about 5-fold more neutrophils in the peritoneal cavity than controls during the first 5 hour post-injection
• detectable amounts of neutrophils are present within 3 hours of LPS injection while neutrophils are not observed in wild-type controls until 24 hours after LPS injection
• neutrophil response can be induced with very low amounts of LPS
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• macrophages are less responsive to LPS compared to controls as determined by TNF secretion
• macrophages are least responsive to carbohydrated (i.e. smooth) forms of LPS where 150,000 fold more is needed to generate the same response as in controls
• in contrast, macrophages are only 4-fold less responsive to the non-carbohydrated form of LPS known as lipid A
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• lower induction by Propionibacterium acnes
• normal induction by peptidoglycan
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• lower induction by Propionibacterium acnes
• normal induction by peptidoglycan
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice are resistant to gram-negative E. coli bacterial infection with 100% survivability when given an inoculum lethal to control mice
• four times the lethal dose of E. coli leads to death in about half the mutant mice, and all the mice die at six times the lethal dose of E. coli
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• mice release substantially less IL-6 into their bloodstream after injection with LPS or when infected with E. coli
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• mice release substantially less TNF into their bloodstream after injection with LPS or when infected with E. coli
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• 100-1000 fold more LPS is needed by monocytes to secrete the same amount of IL-6 as in LPS-dosed controls
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• 100-1000 fold more LPS is needed by monocytes to secrete the same amount of TNF as in LPS-dosed controls
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• none of the mice die when injected with a dose of LPS that causes a 100% lethality in wild-type controls
• when given 10 times the lethal dose of LPS, some of the mutant mice demonstrate signs of mild endotoxemia
• only in mice given a LPS sensitizer and 10 times the lethal dose do mice exhibit some lethality
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• 27-fold lower level of bacteria are found in the blood of these infected mutant mice compared to controls
• in mice receiving six times the lethal dose of E. coli, there are 35-fold fewer bacteria in the blood than in controls 7 hour after infection
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• mice are resistant to gram-negative E. coli bacterial infection with 100% survivability when given an inoculum lethal to control mice
• four times the lethal dose of E. coli leads to death in about half the mutant mice, and all the mice die at six times the lethal dose of E. coli
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• mice release substantially less IL-6 into their bloodstream after injection with LPS or when infected with E. coli
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• mice release substantially less TNF into their bloodstream after injection with LPS or when infected with E. coli
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• macrophages fail to respond to LPS even at the very high doses that stimulate macrophages homozygote for just the CD14tm1Smg null allele
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• no TNF is produced by macrophages in response to LPS stimulation
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• macrophages fail to respond to LPS even at the very high doses that stimulate macrophages homozygote for just the CD14tm1Smg null allele
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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