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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Apaf1Gt(IRESBetageo)XIX18Pgr
gene trap XIX18, Peter Gruss
MGI:1857868
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr involves: 129/Sv * 129X1/SvJ MGI:3610484
hm2
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr involves: 129S1/Sv * 129X1/SvJ MGI:3687282
hm3
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr involves: 129S1/Sv * 129X1/SvJ * NMRI MGI:3588510
ht4
Apaf1fog/Apaf1Gt(IRESBetageo)XIX18Pgr involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C3H/HeJ * NMRI MGI:3783544
cn5
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr
Aifm1tm2Pngr/Y
Foxg1tm1(cre)Skm/Foxg1+
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ MGI:3687281
cx6
Aifm1Hq/Aifm1Hq
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA MGI:3699821


Genotype
MGI:3610484
hm1
Allelic
Composition
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr
Genetic
Background
involves: 129/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apaf1Gt(IRESBetageo)XIX18Pgr mutation (1 available); any Apaf1 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• 5 Gy gamma-irradiation of CD4+CD8+ thymocytes from hosts reconstituted with Apaf1Gt(IRESBetageo)XIX18Pgr homozygous fetal liver do not display caspase 2 cleavage while those derived from wildtype fetal liver do (J:103480)
• 5 Gy gamma-irradiation of CD4+CD8+ thymocytes from hosts reconstituted with Apaf1Gt(IRESBetageo)XIX18Pgr homozygous fetal liver do not display caspase 2 cleavage while those derived from wildtype fetal liver do (J:103480)




Genotype
MGI:3687282
hm2
Allelic
Composition
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apaf1Gt(IRESBetageo)XIX18Pgr mutation (1 available); any Apaf1 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• cultured neurons show increased survival vs wild-type after camptothecin treatment (J:112874)
• cultured neurons show increased survival vs wild-type after camptothecin treatment (J:112874)
• when cultured neurons express anchored form of Pdcd8 as well as endogenous Pdcd8, significant apoptosis is observed (J:112874)
• when cultured neurons express anchored form of Pdcd8 as well as endogenous Pdcd8, significant apoptosis is observed (J:112874)

cellular
• cultured neurons show increased survival vs wild-type after camptothecin treatment (J:112874)
• cultured neurons show increased survival vs wild-type after camptothecin treatment (J:112874)
• when cultured neurons express anchored form of Pdcd8 as well as endogenous Pdcd8, significant apoptosis is observed (J:112874)
• when cultured neurons express anchored form of Pdcd8 as well as endogenous Pdcd8, significant apoptosis is observed (J:112874)




Genotype
MGI:3588510
hm3
Allelic
Composition
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * NMRI
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apaf1Gt(IRESBetageo)XIX18Pgr mutation (1 available); any Apaf1 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• embryonic defects apparent by E12.5, die by E17 (J:49840)
• embryonic defects apparent by E12.5, die by E17 (J:49840)

cellular
• induction of apoptosis in mutant embryonic fibroblasts is similar to controls, but mutant fibroblasts show reduced cell death after prolonged treatment with apoptotic stimuli (anti-Fas antibody, C6-ceramide, and staurosporin) (J:49840)
• induction of apoptosis in mutant embryonic fibroblasts is similar to controls, but mutant fibroblasts show reduced cell death after prolonged treatment with apoptotic stimuli (anti-Fas antibody, C6-ceramide, and staurosporin) (J:49840)

craniofacial
• absence of skull vault (J:49840)
• absence of skull vault (J:49840)
• late and imperfect palatial fusion occurs (J:49840)
• late and imperfect palatial fusion occurs (J:49840)

nervous system
N
• at E13-14 and E18, numbers of spinal motoneurons and dorsal root ganglion neurons are not different from wild-type (J:131954)
• at E13-14 and E18, numbers of spinal motoneurons and dorsal root ganglion neurons are not different from wild-type (J:131954)
• brain hyperplasia presumably due to lack of apoptosis in the mantle layer of the developing diencephalon, midbrain, cerebellum and ventricular layer of the choroid plexus of the fourth ventricle; an excess of differentiating neurons is observed in these locations (J:49840)
• brain hyperplasia presumably due to lack of apoptosis in the mantle layer of the developing diencephalon, midbrain, cerebellum and ventricular layer of the choroid plexus of the fourth ventricle; an excess of differentiating neurons is observed in these locations (J:49840)
• overgrowth resulting in abnormal folding and generation of a mantle layer (J:49840)
• overgrowth resulting in abnormal folding and generation of a mantle layer (J:49840)
• may be due to intense overgrowth (hyperplasia) of diencephalon and midbrain (J:49840)
• may be due to intense overgrowth (hyperplasia) of diencephalon and midbrain (J:49840)
• may be due to intense overgrowth (hyperplasia) of diencephalon and midbrain (J:49840)
• may be due to intense overgrowth (hyperplasia) of diencephalon and midbrain (J:49840)
• rostral exencephaly (J:49840)
• rostral exencephaly (J:49840)
• forebrain exencephaly is observed in all animals (J:131954)
• forebrain exencephaly is observed in all animals (J:131954)
• results from obliteration of the lumen of the neural tube (J:49840)
• results from obliteration of the lumen of the neural tube (J:49840)
• hyperplasia of the choroid plexus of the fourth ventricle is seen (J:49840)
• hyperplasia of the choroid plexus of the fourth ventricle is seen (J:49840)
• abnormal overgrowth of ventral side of hypothalamus through the base of the skull (J:49840)
• abnormal overgrowth of ventral side of hypothalamus through the base of the skull (J:49840)
• at E14 and E18, motoneurons and DRG neurons exhibit degenerative-like changes not seen in wild-type (J:131954)
• at E14 and E18, motoneurons and DRG neurons exhibit degenerative-like changes not seen in wild-type (J:131954)
• at E14, developing neurons undergo atypical programmed cell death (PCD) in contrast to type 1 (apoptotic-like) mechanism exhibited in wild-type neurons; apoptotic-like degeneration markers (such as TUNEL labeling) are not observed in dying mutant neurons (J:131954)
• at E14, developing neurons undergo atypical programmed cell death (PCD) in contrast to type 1 (apoptotic-like) mechanism exhibited in wild-type neurons; apoptotic-like degeneration markers (such as TUNEL labeling) are not observed in dying mutant neurons (J:131954)

vision/eye
• eye vascular endothelial cells obliterate the optic cup at E14.5 (J:49840)
• eye vascular endothelial cells obliterate the optic cup at E14.5 (J:49840)
• by E12.5, the retina is noticeably thicker (J:49840)
• by E14.5, the hyperplastic retina fills the optic cup and is folded (J:49840)
• by E12.5, the retina is noticeably thicker (J:49840)
• by E14.5, the hyperplastic retina fills the optic cup and is folded (J:49840)

limbs/digits/tail
• interdigital webbing in limb buds with reduced apoptosis (J:49840)
• interdigital webbing in limb buds with reduced apoptosis (J:49840)

skeleton
• absence of skull vault (J:49840)
• absence of skull vault (J:49840)

digestive/alimentary system
• late and imperfect palatial fusion occurs (J:49840)
• late and imperfect palatial fusion occurs (J:49840)

embryogenesis
• tissues that normally exhibit apoptosis in developmental stages instead exhibit hyperplasia and/or overgrowth (J:49840)
• tissues that normally exhibit apoptosis in developmental stages instead exhibit hyperplasia and/or overgrowth (J:49840)
• overgrowth resulting in abnormal folding and generation of a mantle layer (J:49840)
• overgrowth resulting in abnormal folding and generation of a mantle layer (J:49840)

growth/size/body
• late and imperfect palatial fusion occurs (J:49840)
• late and imperfect palatial fusion occurs (J:49840)




Genotype
MGI:3783544
ht4
Allelic
Composition
Apaf1fog/Apaf1Gt(IRESBetageo)XIX18Pgr
Genetic
Background
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C3H/HeJ * NMRI
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apaf1fog mutation (1 available); any Apaf1 mutation (14 available)
Apaf1Gt(IRESBetageo)XIX18Pgr mutation (1 available); any Apaf1 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• dying motoneurons are rarely observed at E14 and E18, unlike in wild-type animals (J:131954)
• dying motoneurons are rarely observed at E14 and E18, unlike in wild-type animals (J:131954)
• neurons display characteristics of type 2 (autophagic) programmed cell death, in contrast to type 1 (apoptotic) PCD seen in wild-type (J:131954)
• neurons display characteristics of type 2 (autophagic) programmed cell death, in contrast to type 1 (apoptotic) PCD seen in wild-type (J:131954)
• motoneurons have normal nuclei and atypical cytoplasm, with structures having characteristics of lysosomes; cytoplasmic organelles appear aggregated in area with many lysosomes and autophagosomes (J:131954)
• mitochondria show loss of cristae and swollen rounded appearance; rough endoplasmic reticulum is dilated and Golgi is hypertrophic (J:131954)
• as degeneration proceeds, nucleus and cytoplasm become more condensed; at late stages, nucleus is condensed but remains intact (J:131954)
• motoneurons have normal nuclei and atypical cytoplasm, with structures having characteristics of lysosomes; cytoplasmic organelles appear aggregated in area with many lysosomes and autophagosomes (J:131954)
• mitochondria show loss of cristae and swollen rounded appearance; rough endoplasmic reticulum is dilated and Golgi is hypertrophic (J:131954)
• as degeneration proceeds, nucleus and cytoplasm become more condensed; at late stages, nucleus is condensed but remains intact (J:131954)

cellular
• dying motoneurons are rarely observed at E14 and E18, unlike in wild-type animals (J:131954)
• dying motoneurons are rarely observed at E14 and E18, unlike in wild-type animals (J:131954)




Genotype
MGI:3687281
cn5
Allelic
Composition
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr
Aifm1tm2Pngr/Y
Foxg1tm1(cre)Skm/Foxg1+
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aifm1tm2Pngr mutation (0 available); any Aifm1 mutation (7 available)
Apaf1Gt(IRESBetageo)XIX18Pgr mutation (1 available); any Apaf1 mutation (14 available)
Foxg1tm1(cre)Skm mutation (2 available); any Foxg1 mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• neurons show increased survival vs wild-type after camptothecin treatment (J:112874)
• neurons cultured in pyruvate supplemented media show increased survival after camptothecin treatment (J:112874)
• expression of anchored Pdcd8 does not provide further protection from death to neurons (J:112874)
• neurons show increased survival vs wild-type after camptothecin treatment (J:112874)
• neurons cultured in pyruvate supplemented media show increased survival after camptothecin treatment (J:112874)
• expression of anchored Pdcd8 does not provide further protection from death to neurons (J:112874)
• cultured neurons show increased apoptosis reconstituted with wild-type Pdcd8 compared to control; mutant neurons expressing Pdcd8 with a nuclear exclusion sequence show cell death equivalent to control neurons expressing GFP (J:112874)
• cultured neurons show increased apoptosis reconstituted with wild-type Pdcd8 compared to control; mutant neurons expressing Pdcd8 with a nuclear exclusion sequence show cell death equivalent to control neurons expressing GFP (J:112874)
• cultured neurons can maintain oxygen consumption after camptothecin treatment if anchored Pdcd8 is expressed (J:112874)
• cultured neurons can maintain oxygen consumption after camptothecin treatment if anchored Pdcd8 is expressed (J:112874)

nervous system
• neurons show increased survival vs wild-type after camptothecin treatment (J:112874)
• neurons cultured in pyruvate supplemented media show increased survival after camptothecin treatment (J:112874)
• expression of anchored Pdcd8 does not provide further protection from death to neurons (J:112874)
• neurons show increased survival vs wild-type after camptothecin treatment (J:112874)
• neurons cultured in pyruvate supplemented media show increased survival after camptothecin treatment (J:112874)
• expression of anchored Pdcd8 does not provide further protection from death to neurons (J:112874)
• cultured neurons show increased apoptosis reconstituted with wild-type Pdcd8 compared to control; mutant neurons expressing Pdcd8 with a nuclear exclusion sequence show cell death equivalent to control neurons expressing GFP (J:112874)
• cultured neurons show increased apoptosis reconstituted with wild-type Pdcd8 compared to control; mutant neurons expressing Pdcd8 with a nuclear exclusion sequence show cell death equivalent to control neurons expressing GFP (J:112874)
• cortex is thickened compared to Pdcd8 conditional embryos (J:112874)
• cortex is thickened compared to Pdcd8 conditional embryos (J:112874)




Genotype
MGI:3699821
cx6
Allelic
Composition
Aifm1Hq/Aifm1Hq
Apaf1Gt(IRESBetageo)XIX18Pgr/Apaf1Gt(IRESBetageo)XIX18Pgr
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aifm1Hq mutation (2 available); any Aifm1 mutation (7 available)
Apaf1Gt(IRESBetageo)XIX18Pgr mutation (1 available); any Apaf1 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• reduced apoptosis of cultured cortical neurons resulting from treatment with camptothecin (J:98103)
• neurons show enhanced viability when treated with camptothecin (J:98103)
• only about half of dying cells exhibit DNA fragmentation (J:98103)
• reduced apoptosis of cultured cortical neurons resulting from treatment with camptothecin (J:98103)
• neurons show enhanced viability when treated with camptothecin (J:98103)
• only about half of dying cells exhibit DNA fragmentation (J:98103)

cellular
• reduced apoptosis of cultured cortical neurons resulting from treatment with camptothecin (J:98103)
• neurons show enhanced viability when treated with camptothecin (J:98103)
• only about half of dying cells exhibit DNA fragmentation (J:98103)
• reduced apoptosis of cultured cortical neurons resulting from treatment with camptothecin (J:98103)
• neurons show enhanced viability when treated with camptothecin (J:98103)
• only about half of dying cells exhibit DNA fragmentation (J:98103)





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last database update
02/02/2016
MGI 6.02
The Jackson Laboratory